Profiles of IgE sensitization to Der f 1, Der f 2, Der f 6, Der f 8, Der f 10, and Der f 20 in Korean house dust mite allergy patients

Kyoung Yong Jeong, June Yong Lee, Mina Son, Myung Hee Yi, Tai Soon Yong, Jung U. Shin, Kwang Hoon Lee, Yoon Ju Kim, Kyung Hee Park, Hye Jung Park, Jae Hyun Lee, Jung Won Park

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19 Citations (Scopus)

Abstract

Purpose: Measurement of IgE specific to purified house dust mite (HDM) allergens may improve allergy diagnosis. This study aimed to investigate the sensitization profiles of Korean HDM allergic subjects suffering from respiratory allergy and atopic dermatitis (AD) to Der f 1, Der f 2, Der f 6, Der f 8, Der f 10, and Der f 20. Methods: Recombinant HDM allergens were produced in Pichia pastoris (Der f 1) or Escherichia coli (5 allergens). IgE reactivity to the individual recombinant allergens and total extract of mite was assessed by ELISA. Results: Der f 1 was recognized by 79.1%, Der f 2 by 79.1%, Der f 6 by 9.3%, Der f 8 by 6.2%, Der f 10 by 6.2%, and Der f 20 by 6.6% of the patients' sera tested, while the prevalence of IgE reactivity to total mite extract was 94.7%. Combination of Der f 1 and Der f 2 had a sensitivity of 87.6%. Specific IgE to Der f 2 alone was detected from 89.4% of HDM-sensitized respiratory allergy subjects and 92.3% to the combination of the 2 major allergens Der f 1 and Der f 2. However, sera from fewer patients with AD, namely 72.4% and 71.0%, recognized Der f 1 and Der f 2, respectively. The combination of 2 major allergens allowed diagnosis of 84.5% of the AD patients. No correlation between sensitization to specific allergens and HDM allergy entity was found. Conclusions: Der f 2 was the most frequently sensitized allergen among the HDM-sensitized respiratory and AD patients in Korea, and the combination of the group 1 and 2 major allergens increased the diagnostic sensitivity. Minor allergens did not significantly improve diagnostic sensitivity. However, further studies are needed to analyze the relationship between sensitization to other HDM allergens and the disease entity of the HDM allergy.

Original languageEnglish
Pages (from-to)483-488
Number of pages6
JournalAllergy, Asthma and Immunology Research
Volume7
Issue number5
DOIs
Publication statusPublished - 2015 Jan 1

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Pyroglyphidae
Dermatophagoides Antigens
Immunoglobulin E
Hypersensitivity
Allergens
Atopic Dermatitis
Mites
Pichia
Korea
Dermatophagoides farinae antigen f 2
Dermatophagoides farinae antigen f 1
Serum
Enzyme-Linked Immunosorbent Assay
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Pulmonary and Respiratory Medicine

Cite this

Jeong, Kyoung Yong ; Lee, June Yong ; Son, Mina ; Yi, Myung Hee ; Yong, Tai Soon ; Shin, Jung U. ; Lee, Kwang Hoon ; Kim, Yoon Ju ; Park, Kyung Hee ; Park, Hye Jung ; Lee, Jae Hyun ; Park, Jung Won. / Profiles of IgE sensitization to Der f 1, Der f 2, Der f 6, Der f 8, Der f 10, and Der f 20 in Korean house dust mite allergy patients. In: Allergy, Asthma and Immunology Research. 2015 ; Vol. 7, No. 5. pp. 483-488.
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title = "Profiles of IgE sensitization to Der f 1, Der f 2, Der f 6, Der f 8, Der f 10, and Der f 20 in Korean house dust mite allergy patients",
abstract = "Purpose: Measurement of IgE specific to purified house dust mite (HDM) allergens may improve allergy diagnosis. This study aimed to investigate the sensitization profiles of Korean HDM allergic subjects suffering from respiratory allergy and atopic dermatitis (AD) to Der f 1, Der f 2, Der f 6, Der f 8, Der f 10, and Der f 20. Methods: Recombinant HDM allergens were produced in Pichia pastoris (Der f 1) or Escherichia coli (5 allergens). IgE reactivity to the individual recombinant allergens and total extract of mite was assessed by ELISA. Results: Der f 1 was recognized by 79.1{\%}, Der f 2 by 79.1{\%}, Der f 6 by 9.3{\%}, Der f 8 by 6.2{\%}, Der f 10 by 6.2{\%}, and Der f 20 by 6.6{\%} of the patients' sera tested, while the prevalence of IgE reactivity to total mite extract was 94.7{\%}. Combination of Der f 1 and Der f 2 had a sensitivity of 87.6{\%}. Specific IgE to Der f 2 alone was detected from 89.4{\%} of HDM-sensitized respiratory allergy subjects and 92.3{\%} to the combination of the 2 major allergens Der f 1 and Der f 2. However, sera from fewer patients with AD, namely 72.4{\%} and 71.0{\%}, recognized Der f 1 and Der f 2, respectively. The combination of 2 major allergens allowed diagnosis of 84.5{\%} of the AD patients. No correlation between sensitization to specific allergens and HDM allergy entity was found. Conclusions: Der f 2 was the most frequently sensitized allergen among the HDM-sensitized respiratory and AD patients in Korea, and the combination of the group 1 and 2 major allergens increased the diagnostic sensitivity. Minor allergens did not significantly improve diagnostic sensitivity. However, further studies are needed to analyze the relationship between sensitization to other HDM allergens and the disease entity of the HDM allergy.",
author = "Jeong, {Kyoung Yong} and Lee, {June Yong} and Mina Son and Yi, {Myung Hee} and Yong, {Tai Soon} and Shin, {Jung U.} and Lee, {Kwang Hoon} and Kim, {Yoon Ju} and Park, {Kyung Hee} and Park, {Hye Jung} and Lee, {Jae Hyun} and Park, {Jung Won}",
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Profiles of IgE sensitization to Der f 1, Der f 2, Der f 6, Der f 8, Der f 10, and Der f 20 in Korean house dust mite allergy patients. / Jeong, Kyoung Yong; Lee, June Yong; Son, Mina; Yi, Myung Hee; Yong, Tai Soon; Shin, Jung U.; Lee, Kwang Hoon; Kim, Yoon Ju; Park, Kyung Hee; Park, Hye Jung; Lee, Jae Hyun; Park, Jung Won.

In: Allergy, Asthma and Immunology Research, Vol. 7, No. 5, 01.01.2015, p. 483-488.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Profiles of IgE sensitization to Der f 1, Der f 2, Der f 6, Der f 8, Der f 10, and Der f 20 in Korean house dust mite allergy patients

AU - Jeong, Kyoung Yong

AU - Lee, June Yong

AU - Son, Mina

AU - Yi, Myung Hee

AU - Yong, Tai Soon

AU - Shin, Jung U.

AU - Lee, Kwang Hoon

AU - Kim, Yoon Ju

AU - Park, Kyung Hee

AU - Park, Hye Jung

AU - Lee, Jae Hyun

AU - Park, Jung Won

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Purpose: Measurement of IgE specific to purified house dust mite (HDM) allergens may improve allergy diagnosis. This study aimed to investigate the sensitization profiles of Korean HDM allergic subjects suffering from respiratory allergy and atopic dermatitis (AD) to Der f 1, Der f 2, Der f 6, Der f 8, Der f 10, and Der f 20. Methods: Recombinant HDM allergens were produced in Pichia pastoris (Der f 1) or Escherichia coli (5 allergens). IgE reactivity to the individual recombinant allergens and total extract of mite was assessed by ELISA. Results: Der f 1 was recognized by 79.1%, Der f 2 by 79.1%, Der f 6 by 9.3%, Der f 8 by 6.2%, Der f 10 by 6.2%, and Der f 20 by 6.6% of the patients' sera tested, while the prevalence of IgE reactivity to total mite extract was 94.7%. Combination of Der f 1 and Der f 2 had a sensitivity of 87.6%. Specific IgE to Der f 2 alone was detected from 89.4% of HDM-sensitized respiratory allergy subjects and 92.3% to the combination of the 2 major allergens Der f 1 and Der f 2. However, sera from fewer patients with AD, namely 72.4% and 71.0%, recognized Der f 1 and Der f 2, respectively. The combination of 2 major allergens allowed diagnosis of 84.5% of the AD patients. No correlation between sensitization to specific allergens and HDM allergy entity was found. Conclusions: Der f 2 was the most frequently sensitized allergen among the HDM-sensitized respiratory and AD patients in Korea, and the combination of the group 1 and 2 major allergens increased the diagnostic sensitivity. Minor allergens did not significantly improve diagnostic sensitivity. However, further studies are needed to analyze the relationship between sensitization to other HDM allergens and the disease entity of the HDM allergy.

AB - Purpose: Measurement of IgE specific to purified house dust mite (HDM) allergens may improve allergy diagnosis. This study aimed to investigate the sensitization profiles of Korean HDM allergic subjects suffering from respiratory allergy and atopic dermatitis (AD) to Der f 1, Der f 2, Der f 6, Der f 8, Der f 10, and Der f 20. Methods: Recombinant HDM allergens were produced in Pichia pastoris (Der f 1) or Escherichia coli (5 allergens). IgE reactivity to the individual recombinant allergens and total extract of mite was assessed by ELISA. Results: Der f 1 was recognized by 79.1%, Der f 2 by 79.1%, Der f 6 by 9.3%, Der f 8 by 6.2%, Der f 10 by 6.2%, and Der f 20 by 6.6% of the patients' sera tested, while the prevalence of IgE reactivity to total mite extract was 94.7%. Combination of Der f 1 and Der f 2 had a sensitivity of 87.6%. Specific IgE to Der f 2 alone was detected from 89.4% of HDM-sensitized respiratory allergy subjects and 92.3% to the combination of the 2 major allergens Der f 1 and Der f 2. However, sera from fewer patients with AD, namely 72.4% and 71.0%, recognized Der f 1 and Der f 2, respectively. The combination of 2 major allergens allowed diagnosis of 84.5% of the AD patients. No correlation between sensitization to specific allergens and HDM allergy entity was found. Conclusions: Der f 2 was the most frequently sensitized allergen among the HDM-sensitized respiratory and AD patients in Korea, and the combination of the group 1 and 2 major allergens increased the diagnostic sensitivity. Minor allergens did not significantly improve diagnostic sensitivity. However, further studies are needed to analyze the relationship between sensitization to other HDM allergens and the disease entity of the HDM allergy.

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