Profiling of human urinary phospholipids by nanoflow liquid chromatography/tandem mass spectrometry

Hanna Kim, Eunjeong Ahn, Hee Moon Myeong

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Nanoflow liquid chromatography-electrospray ionization-tandem mass spectrometry (nanoLC-ESI-MS-MS) was used for the first time in a comprehensive analysis of human urinary phospholipids (PL). PL mixtures from human urine were separated with a reversed phase LC capillary column coupled to ESI-MS-MS. This study used the dual scan method in which two consecutive LC-ESI-MS-MS runs were done in both positive ion mode to detect phosphatidylcholine (PC) and phosphatidylethanolamine (PE), and in negative ion mode to detect phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidic acid (PA), and phosphatidylglycerol (PG). We focused on identifying the maximum number of PLs from a healthy human urine sample by varying the extracted volume of urine along with the evaluation of extraction efficiency for urinary PLs. We found that 22 PCs, 14 PEs, 15 PIs, 13 PSs, 7 PAs, and 4 PGs were identified during nLC-ESI-MS-MS when phospholipids in urine were extracted by ultracentrifugation. The efficiency of lipid extraction by ultracentrifugation versus lyophilization was evaluated by reducing the initial urine volume. We found that lyophilization was more efficient than ultracentrifugation for extracting lipids from small volumes (1 mL) of urine.

Original languageEnglish
Pages (from-to)1656-1663
Number of pages8
JournalAnalyst
Volume133
Issue number12
DOIs
Publication statusPublished - 2008 Nov 27

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Phospholipids
Liquid chromatography
Tandem Mass Spectrometry
phospholipid
Liquid Chromatography
urine
Mass spectrometry
liquid chromatography
mass spectrometry
Urine
Lipids
Ultracentrifugation
Electrospray ionization
Phosphatidylglycerols
Phosphatidic Acids
Freeze Drying
Phosphatidylserines
Phosphatidylinositols
Phosphatidylcholines
Negative ions

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

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abstract = "Nanoflow liquid chromatography-electrospray ionization-tandem mass spectrometry (nanoLC-ESI-MS-MS) was used for the first time in a comprehensive analysis of human urinary phospholipids (PL). PL mixtures from human urine were separated with a reversed phase LC capillary column coupled to ESI-MS-MS. This study used the dual scan method in which two consecutive LC-ESI-MS-MS runs were done in both positive ion mode to detect phosphatidylcholine (PC) and phosphatidylethanolamine (PE), and in negative ion mode to detect phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidic acid (PA), and phosphatidylglycerol (PG). We focused on identifying the maximum number of PLs from a healthy human urine sample by varying the extracted volume of urine along with the evaluation of extraction efficiency for urinary PLs. We found that 22 PCs, 14 PEs, 15 PIs, 13 PSs, 7 PAs, and 4 PGs were identified during nLC-ESI-MS-MS when phospholipids in urine were extracted by ultracentrifugation. The efficiency of lipid extraction by ultracentrifugation versus lyophilization was evaluated by reducing the initial urine volume. We found that lyophilization was more efficient than ultracentrifugation for extracting lipids from small volumes (1 mL) of urine.",
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Profiling of human urinary phospholipids by nanoflow liquid chromatography/tandem mass spectrometry. / Kim, Hanna; Ahn, Eunjeong; Myeong, Hee Moon.

In: Analyst, Vol. 133, No. 12, 27.11.2008, p. 1656-1663.

Research output: Contribution to journalArticle

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