Profiling of phospholipids in lipoproteins by multiplexed hollow fiber flow field-flow fractionation and nanoflow liquid chromatography-tandem mass spectrometry

Ju Yong Lee, Hye Kyeong Min, Donghoon Choi, Myeong Hee Moon

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

This study describes a coupled analytical method to carry out the systematic profiling of phospholipids (PLs) in high-density lipoproteins (HDL) and low-density lipoproteins (LDL) from human blood plasma. HDL and LDL of healthy human plasma samples were separated by size and collected on a semi-preparative scale using multiplexed hollow fiber flow field-flow fractionation (MxHF5). Phospholipid mixtures contained in the resulting HDL and LDL fractions were analyzed by shotgun nanoflow liquid chromatography-tandem mass spectrometry (nLC-ESI-MS-MS). We utilized a dual scan method for the separation and simultaneous characterization of complicated PL mixtures by nLC-ESI-MS-MS, such that phosphatidylcholine (PC) and phosphatidylethanolamine (PE) molecules were detected in positive ion mode in a first LC run. In a second LC run, phosphatidylinositol (PI), phosphatidylglycerol (PG), and phosphatidic acid (PA) were detected in negative ion mode. In this study, a total of 56 PLs from HDL and 52 PLs from LDL particles were characterized by their molecular structures from data dependent collision-induced dissociation (CID) experiments, and their relative abundances were compared.

Original languageEnglish
Pages (from-to)1660-1666
Number of pages7
JournalJournal of Chromatography A
Volume1217
Issue number10
DOIs
Publication statusPublished - 2010 Mar 5

Bibliographical note

Funding Information:
This work was supported by a Korea Research Foundation Grant ( KRF-2008-313-C00567 ) and a grant from the Korea Science and Engineering Foundation (KOSEF) through the Center for Bioactive Molecular Hybrids (CBMH).

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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