Programmed cell death 5 suppresses AKT-mediated cytoprotection of endothelium

Seung Hyun Lee; Jaesung Seo; Soo Yeon Park; Mi Hyeon Jeong; Hyo Kyoung Choi; Chan Joo Lee; Mi Jeong Kim; Garam Guk; Soo Yeon Lee; Hyewon Park; Jae Wook Jeong; Chang Hoon Ha; Sungha Park; Ho Geun Yoon

doi:10.1073/pnas.1712918115

Programmed cell death 5 suppresses AKT-mediated cytoprotection of endothelium

Seung Hyun Lee, Jaesung Seo, Soo Yeon Park, Mi Hyeon Jeong, Hyo Kyoung Choi, Chan Joo Lee, Mi Jeong Kim, Garam Guk, Soo Yeon Lee, Hyewon Park, Jae Wook Jeong, Chang Hoon Ha, Sungha Park, Ho Geun Yoon

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Programmed cell death 5 (PDCD5) has been associated with human cancers as a regulator of cell death; however, the role of PDCD5 in the endothelium has not been revealed. Thus, we investigated whether PDCD5 regulates protein kinase B (PKB/AKT)-endothelial nitric oxide synthase (eNOS)–dependent signal transduction in the endothelium and affects atherosclerosis. Endothelial-specific PDCD5 knockout mice showed significantly reduced vascular remodeling compared with wild-type (WT) mice after partial carotid ligation. WT PDCD5 competitively inhibited interaction between histone deacetylase 3 (HDAC3) and AKT, but PDCD5^L6R, an HDAC3-binding–deficient mutant, did not. Knockdown of PDCD5 accelerated HDAC3–AKT interaction, AKT and eNOS phosphorylation, and nitric oxide (NO) production in human umbilical vein endothelial cells. Moreover, we found that serum PDCD5 levels reflect endothelial NO production and are correlated with diabetes mellitus, high-density lipoprotein cholesterol, and coronary calcium in human samples obtained from the cardiovascular high-risk cohort. Therefore, we conclude that PDCD5 is associated with endothelial dysfunction and may be a novel therapeutic target in atherosclerosis.

Original language	English
Pages (from-to)	4672-4677
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	115
Issue number	18
DOIs	https://doi.org/10.1073/pnas.1712918115
Publication status	Published - 2018 May 1

Bibliographical note

Funding Information:
We thank Dong-Su Jang for providing illustrations and Changsoo Kim (Yonsei University College of Medicine) for reviewing our statistical analyses. This work was supported by the Basic Science Research Program of the National Research Foundation of Korea, funded by the Korean Government through Grants NRF-2017R1E1A1A01072732, NRF-2015R1A2A1A05000899, and NRF-2011-0030086 to H.-G.Y. and NRF-2015R1A2A2A01007346 to S.P. This work was also supported by the Korean Health Technology R&D Project through the Korean Health Industry Development Institute, funded by the Ministry of Health and Welfare, Republic of Korea (Grants HI13C0715 and HI08C2149, to S.P.).

Funding Information:
ACKNOWLEDGMENTS. We thank Dong-Su Jang for providing illustrations and Changsoo Kim (Yonsei University College of Medicine) for reviewing our statistical analyses. This work was supported by the Basic Science Research Program of the National Research Foundation of Korea, funded by the Korean Government through Grants NRF-2017R1E1A1A01072732, NRF-2015R1A2A1A05000899, and NRF-2011-0030086 to H.-G.Y. and NRF-2015R1A2A2A01007346 to S.P. This work was also supported by the Korean Health Technology R&D Project through the Korean Health Industry Development Institute, funded by the Ministry of Health and Welfare, Republic of Korea (Grants HI13C0715 and HI08C2149, to S.P.).

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© 2018 National Academy of Sciences. All rights reserved.

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Lee, S. H., Seo, J., Park, S. Y., Jeong, M. H., Choi, H. K., Lee, C. J., Kim, M. J., Guk, G., Lee, S. Y., Park, H., Jeong, J. W., Ha, C. H., Park, S., & Yoon, H. G. (2018). Programmed cell death 5 suppresses AKT-mediated cytoprotection of endothelium. Proceedings of the National Academy of Sciences of the United States of America, 115(18), 4672-4677. https://doi.org/10.1073/pnas.1712918115

Lee, Seung Hyun ; Seo, Jaesung ; Park, Soo Yeon ; Jeong, Mi Hyeon ; Choi, Hyo Kyoung ; Lee, Chan Joo ; Kim, Mi Jeong ; Guk, Garam ; Lee, Soo Yeon ; Park, Hyewon ; Jeong, Jae Wook ; Ha, Chang Hoon ; Park, Sungha ; Yoon, Ho Geun. / Programmed cell death 5 suppresses AKT-mediated cytoprotection of endothelium. In: Proceedings of the National Academy of Sciences of the United States of America. 2018 ; Vol. 115, No. 18. pp. 4672-4677.

@article{441dbb4d1ede4176b01e21ced9424414,

title = "Programmed cell death 5 suppresses AKT-mediated cytoprotection of endothelium",

abstract = "Programmed cell death 5 (PDCD5) has been associated with human cancers as a regulator of cell death; however, the role of PDCD5 in the endothelium has not been revealed. Thus, we investigated whether PDCD5 regulates protein kinase B (PKB/AKT)-endothelial nitric oxide synthase (eNOS)–dependent signal transduction in the endothelium and affects atherosclerosis. Endothelial-specific PDCD5 knockout mice showed significantly reduced vascular remodeling compared with wild-type (WT) mice after partial carotid ligation. WT PDCD5 competitively inhibited interaction between histone deacetylase 3 (HDAC3) and AKT, but PDCD5L6R, an HDAC3-binding–deficient mutant, did not. Knockdown of PDCD5 accelerated HDAC3–AKT interaction, AKT and eNOS phosphorylation, and nitric oxide (NO) production in human umbilical vein endothelial cells. Moreover, we found that serum PDCD5 levels reflect endothelial NO production and are correlated with diabetes mellitus, high-density lipoprotein cholesterol, and coronary calcium in human samples obtained from the cardiovascular high-risk cohort. Therefore, we conclude that PDCD5 is associated with endothelial dysfunction and may be a novel therapeutic target in atherosclerosis.",

author = "Lee, {Seung Hyun} and Jaesung Seo and Park, {Soo Yeon} and Jeong, {Mi Hyeon} and Choi, {Hyo Kyoung} and Lee, {Chan Joo} and Kim, {Mi Jeong} and Garam Guk and Lee, {Soo Yeon} and Hyewon Park and Jeong, {Jae Wook} and Ha, {Chang Hoon} and Sungha Park and Yoon, {Ho Geun}",

note = "Funding Information: We thank Dong-Su Jang for providing illustrations and Changsoo Kim (Yonsei University College of Medicine) for reviewing our statistical analyses. This work was supported by the Basic Science Research Program of the National Research Foundation of Korea, funded by the Korean Government through Grants NRF-2017R1E1A1A01072732, NRF-2015R1A2A1A05000899, and NRF-2011-0030086 to H.-G.Y. and NRF-2015R1A2A2A01007346 to S.P. This work was also supported by the Korean Health Technology R&D Project through the Korean Health Industry Development Institute, funded by the Ministry of Health and Welfare, Republic of Korea (Grants HI13C0715 and HI08C2149, to S.P.). Funding Information: ACKNOWLEDGMENTS. We thank Dong-Su Jang for providing illustrations and Changsoo Kim (Yonsei University College of Medicine) for reviewing our statistical analyses. This work was supported by the Basic Science Research Program of the National Research Foundation of Korea, funded by the Korean Government through Grants NRF-2017R1E1A1A01072732, NRF-2015R1A2A1A05000899, and NRF-2011-0030086 to H.-G.Y. and NRF-2015R1A2A2A01007346 to S.P. This work was also supported by the Korean Health Technology R&D Project through the Korean Health Industry Development Institute, funded by the Ministry of Health and Welfare, Republic of Korea (Grants HI13C0715 and HI08C2149, to S.P.). Publisher Copyright: {\textcopyright} 2018 National Academy of Sciences. All rights reserved.",

year = "2018",

month = may,

day = "1",

doi = "10.1073/pnas.1712918115",

language = "English",

volume = "115",

pages = "4672--4677",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

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Lee, SH, Seo, J, Park, SY, Jeong, MH, Choi, HK, Lee, CJ, Kim, MJ, Guk, G, Lee, SY, Park, H, Jeong, JW, Ha, CH, Park, S & Yoon, HG 2018, 'Programmed cell death 5 suppresses AKT-mediated cytoprotection of endothelium', Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 18, pp. 4672-4677. https://doi.org/10.1073/pnas.1712918115

Programmed cell death 5 suppresses AKT-mediated cytoprotection of endothelium. / Lee, Seung Hyun; Seo, Jaesung; Park, Soo Yeon; Jeong, Mi Hyeon; Choi, Hyo Kyoung; Lee, Chan Joo; Kim, Mi Jeong; Guk, Garam; Lee, Soo Yeon; Park, Hyewon; Jeong, Jae Wook; Ha, Chang Hoon; Park, Sungha; Yoon, Ho Geun.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, No. 18, 01.05.2018, p. 4672-4677.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Programmed cell death 5 suppresses AKT-mediated cytoprotection of endothelium

AU - Lee, Seung Hyun

AU - Seo, Jaesung

AU - Park, Soo Yeon

AU - Jeong, Mi Hyeon

AU - Choi, Hyo Kyoung

AU - Lee, Chan Joo

AU - Kim, Mi Jeong

AU - Guk, Garam

AU - Lee, Soo Yeon

AU - Park, Hyewon

AU - Jeong, Jae Wook

AU - Ha, Chang Hoon

AU - Park, Sungha

AU - Yoon, Ho Geun

N1 - Funding Information: We thank Dong-Su Jang for providing illustrations and Changsoo Kim (Yonsei University College of Medicine) for reviewing our statistical analyses. This work was supported by the Basic Science Research Program of the National Research Foundation of Korea, funded by the Korean Government through Grants NRF-2017R1E1A1A01072732, NRF-2015R1A2A1A05000899, and NRF-2011-0030086 to H.-G.Y. and NRF-2015R1A2A2A01007346 to S.P. This work was also supported by the Korean Health Technology R&D Project through the Korean Health Industry Development Institute, funded by the Ministry of Health and Welfare, Republic of Korea (Grants HI13C0715 and HI08C2149, to S.P.). Funding Information: ACKNOWLEDGMENTS. We thank Dong-Su Jang for providing illustrations and Changsoo Kim (Yonsei University College of Medicine) for reviewing our statistical analyses. This work was supported by the Basic Science Research Program of the National Research Foundation of Korea, funded by the Korean Government through Grants NRF-2017R1E1A1A01072732, NRF-2015R1A2A1A05000899, and NRF-2011-0030086 to H.-G.Y. and NRF-2015R1A2A2A01007346 to S.P. This work was also supported by the Korean Health Technology R&D Project through the Korean Health Industry Development Institute, funded by the Ministry of Health and Welfare, Republic of Korea (Grants HI13C0715 and HI08C2149, to S.P.). Publisher Copyright: © 2018 National Academy of Sciences. All rights reserved.

PY - 2018/5/1

Y1 - 2018/5/1

N2 - Programmed cell death 5 (PDCD5) has been associated with human cancers as a regulator of cell death; however, the role of PDCD5 in the endothelium has not been revealed. Thus, we investigated whether PDCD5 regulates protein kinase B (PKB/AKT)-endothelial nitric oxide synthase (eNOS)–dependent signal transduction in the endothelium and affects atherosclerosis. Endothelial-specific PDCD5 knockout mice showed significantly reduced vascular remodeling compared with wild-type (WT) mice after partial carotid ligation. WT PDCD5 competitively inhibited interaction between histone deacetylase 3 (HDAC3) and AKT, but PDCD5L6R, an HDAC3-binding–deficient mutant, did not. Knockdown of PDCD5 accelerated HDAC3–AKT interaction, AKT and eNOS phosphorylation, and nitric oxide (NO) production in human umbilical vein endothelial cells. Moreover, we found that serum PDCD5 levels reflect endothelial NO production and are correlated with diabetes mellitus, high-density lipoprotein cholesterol, and coronary calcium in human samples obtained from the cardiovascular high-risk cohort. Therefore, we conclude that PDCD5 is associated with endothelial dysfunction and may be a novel therapeutic target in atherosclerosis.

AB - Programmed cell death 5 (PDCD5) has been associated with human cancers as a regulator of cell death; however, the role of PDCD5 in the endothelium has not been revealed. Thus, we investigated whether PDCD5 regulates protein kinase B (PKB/AKT)-endothelial nitric oxide synthase (eNOS)–dependent signal transduction in the endothelium and affects atherosclerosis. Endothelial-specific PDCD5 knockout mice showed significantly reduced vascular remodeling compared with wild-type (WT) mice after partial carotid ligation. WT PDCD5 competitively inhibited interaction between histone deacetylase 3 (HDAC3) and AKT, but PDCD5L6R, an HDAC3-binding–deficient mutant, did not. Knockdown of PDCD5 accelerated HDAC3–AKT interaction, AKT and eNOS phosphorylation, and nitric oxide (NO) production in human umbilical vein endothelial cells. Moreover, we found that serum PDCD5 levels reflect endothelial NO production and are correlated with diabetes mellitus, high-density lipoprotein cholesterol, and coronary calcium in human samples obtained from the cardiovascular high-risk cohort. Therefore, we conclude that PDCD5 is associated with endothelial dysfunction and may be a novel therapeutic target in atherosclerosis.

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DO - 10.1073/pnas.1712918115

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

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IS - 18

ER -

Programmed cell death 5 suppresses AKT-mediated cytoprotection of endothelium

Abstract

Bibliographical note

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