Abstract
Prohibitin, which consists of two subunits PHB1 and PHB2, plays a role in cell-cycle progression, senescence, apoptosis, and maintenance of mitochondrial function in mammals and yeast. In this study, we examined the role of prohibitins in plants by using virus-induced gene silencing (VIGS) of two prohibitin subunit genes of Nicotiana benthamiana, designated NbPHB1 and NbPHB2. NbPHB1 and NbPHB2 were targeted to the mitochondria, and their gene expression was suppressed during senescence. VIGS of NbPHB2 caused severe growth inhibition, leaf yellowing and symptoms of cell death, whereas VIGS of NbPHB1 resulted in a milder phenotype. At the cellular level, depletion of these subunits affected mitochondria by severely reducing their number and/or mass, and by causing morphological and physiological abnormalities. Suppression of prohibitin function resulted in a 10- to 20-fold higher production of reactive oxygen species and induced premature leaf senescence. Finally, disruption of prohibitin function rendered the plants more susceptible to various oxidative stress-inducing reagents, including H2O2, paraquat, antimycin A and salicylic acid. These results suggest that prohibitins play a crucial role in mitochondrial biogenesis and protection against stress and senescence in plant cells.
| Original language | English |
|---|---|
| Pages (from-to) | 658-667 |
| Number of pages | 10 |
| Journal | Plant Journal |
| Volume | 46 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2006 May 1 |
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All Science Journal Classification (ASJC) codes
- Genetics
- Plant Science
- Cell Biology
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Prohibitin is involved in mitochondrial biogenesis in plants. / Ahn, Chang Sook; Lee, Jeong Hee; Reum Hwang, A.; Kim, Woo Taek; Pai, Hyun Sook.
In: Plant Journal, Vol. 46, No. 4, 01.05.2006, p. 658-667.Research output: Contribution to journal › Article
TY - JOUR
T1 - Prohibitin is involved in mitochondrial biogenesis in plants
AU - Ahn, Chang Sook
AU - Lee, Jeong Hee
AU - Reum Hwang, A.
AU - Kim, Woo Taek
AU - Pai, Hyun Sook
PY - 2006/5/1
Y1 - 2006/5/1
N2 - Prohibitin, which consists of two subunits PHB1 and PHB2, plays a role in cell-cycle progression, senescence, apoptosis, and maintenance of mitochondrial function in mammals and yeast. In this study, we examined the role of prohibitins in plants by using virus-induced gene silencing (VIGS) of two prohibitin subunit genes of Nicotiana benthamiana, designated NbPHB1 and NbPHB2. NbPHB1 and NbPHB2 were targeted to the mitochondria, and their gene expression was suppressed during senescence. VIGS of NbPHB2 caused severe growth inhibition, leaf yellowing and symptoms of cell death, whereas VIGS of NbPHB1 resulted in a milder phenotype. At the cellular level, depletion of these subunits affected mitochondria by severely reducing their number and/or mass, and by causing morphological and physiological abnormalities. Suppression of prohibitin function resulted in a 10- to 20-fold higher production of reactive oxygen species and induced premature leaf senescence. Finally, disruption of prohibitin function rendered the plants more susceptible to various oxidative stress-inducing reagents, including H2O2, paraquat, antimycin A and salicylic acid. These results suggest that prohibitins play a crucial role in mitochondrial biogenesis and protection against stress and senescence in plant cells.
AB - Prohibitin, which consists of two subunits PHB1 and PHB2, plays a role in cell-cycle progression, senescence, apoptosis, and maintenance of mitochondrial function in mammals and yeast. In this study, we examined the role of prohibitins in plants by using virus-induced gene silencing (VIGS) of two prohibitin subunit genes of Nicotiana benthamiana, designated NbPHB1 and NbPHB2. NbPHB1 and NbPHB2 were targeted to the mitochondria, and their gene expression was suppressed during senescence. VIGS of NbPHB2 caused severe growth inhibition, leaf yellowing and symptoms of cell death, whereas VIGS of NbPHB1 resulted in a milder phenotype. At the cellular level, depletion of these subunits affected mitochondria by severely reducing their number and/or mass, and by causing morphological and physiological abnormalities. Suppression of prohibitin function resulted in a 10- to 20-fold higher production of reactive oxygen species and induced premature leaf senescence. Finally, disruption of prohibitin function rendered the plants more susceptible to various oxidative stress-inducing reagents, including H2O2, paraquat, antimycin A and salicylic acid. These results suggest that prohibitins play a crucial role in mitochondrial biogenesis and protection against stress and senescence in plant cells.
UR - http://www.scopus.com/inward/record.url?scp=33646235886&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646235886&partnerID=8YFLogxK
U2 - 10.1111/j.1365-313X.2006.02726.x
DO - 10.1111/j.1365-313X.2006.02726.x
M3 - Article
C2 - 16640602
AN - SCOPUS:33646235886
VL - 46
SP - 658
EP - 667
JO - Plant Journal
JF - Plant Journal
SN - 0960-7412
IS - 4
ER -