Prohibitin is involved in mitochondrial biogenesis in plants

Chang Sook Ahn, Jeong Hee Lee, A. Reum Hwang, Woo Taek Kim, Hyun Sook Pai

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Prohibitin, which consists of two subunits PHB1 and PHB2, plays a role in cell-cycle progression, senescence, apoptosis, and maintenance of mitochondrial function in mammals and yeast. In this study, we examined the role of prohibitins in plants by using virus-induced gene silencing (VIGS) of two prohibitin subunit genes of Nicotiana benthamiana, designated NbPHB1 and NbPHB2. NbPHB1 and NbPHB2 were targeted to the mitochondria, and their gene expression was suppressed during senescence. VIGS of NbPHB2 caused severe growth inhibition, leaf yellowing and symptoms of cell death, whereas VIGS of NbPHB1 resulted in a milder phenotype. At the cellular level, depletion of these subunits affected mitochondria by severely reducing their number and/or mass, and by causing morphological and physiological abnormalities. Suppression of prohibitin function resulted in a 10- to 20-fold higher production of reactive oxygen species and induced premature leaf senescence. Finally, disruption of prohibitin function rendered the plants more susceptible to various oxidative stress-inducing reagents, including H2O2, paraquat, antimycin A and salicylic acid. These results suggest that prohibitins play a crucial role in mitochondrial biogenesis and protection against stress and senescence in plant cells.

Original languageEnglish
Pages (from-to)658-667
Number of pages10
JournalPlant Journal
Volume46
Issue number4
DOIs
Publication statusPublished - 2006 May 1

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Organelle Biogenesis
gene silencing
viruses
Gene Silencing
mitochondria
antimycin A
Nicotiana benthamiana
paraquat
salicylic acid
growth retardation
signs and symptoms (plants)
cell death
leaves
reactive oxygen species
cell cycle
Mitochondria
oxidative stress
apoptosis
mammals
yeasts

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Ahn, Chang Sook ; Lee, Jeong Hee ; Reum Hwang, A. ; Kim, Woo Taek ; Pai, Hyun Sook. / Prohibitin is involved in mitochondrial biogenesis in plants. In: Plant Journal. 2006 ; Vol. 46, No. 4. pp. 658-667.
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Prohibitin is involved in mitochondrial biogenesis in plants. / Ahn, Chang Sook; Lee, Jeong Hee; Reum Hwang, A.; Kim, Woo Taek; Pai, Hyun Sook.

In: Plant Journal, Vol. 46, No. 4, 01.05.2006, p. 658-667.

Research output: Contribution to journalArticle

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