Proinflammatory Effects of Calprotectin in Graves’ Orbitopathy

Ji Won Kim, Jae Sang Ko, Jin Joo Kim, Jinsook Yoon

Research output: Contribution to journalArticle

Abstract

Purpose: Early detection and control of inflammation are important to manage Graves’ orbitopathy (GO). We investigated the effects of calprotectin (S100A8/A9) on orbital fibroblast inflammation and GO pathogenesis. Methods: We measured serum calprotectin, S100A8 and S100A9 mRNA expression in orbital fat/connective tissue from GO patients and healthy controls, and proinflammatory cytokines in primary cultured orbital fibroblasts. Results: The serum levels of S100A8/A9 and the expression of S100A8/A9 mRNA in orbital tissue were higher in the GO patients than in the healthy controls. The serum calprotectin levels positively correlated with the clinical activity score and serum thyroid-stimulating immunoglobulin levels. In cultured GO orbital fibroblasts, S100A8/A9 increased the expression of interleukin (IL)-6, IL-8, and monocyte chemotactic protein-1, as well as the phosphorylation of extracellular signal-regulated kinase and nuclear factor-κB. Conclusion: We demonstrated the potential of calprotectin as a biomarker of GO severity and proinflammatory responses to S100A8/A9 in GO orbital fibroblasts.

Original languageEnglish
JournalOcular Immunology and Inflammation
DOIs
Publication statusAccepted/In press - 2018 Jan 1

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Leukocyte L1 Antigen Complex
Fibroblasts
Serum
Thyroid-Stimulating Immunoglobulins
Inflammation
Messenger RNA
Chemokine CCL2
Extracellular Signal-Regulated MAP Kinases
Interleukin-8
Connective Tissue
Interleukin-6
Biomarkers
Fats
Phosphorylation
Cytokines

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Ophthalmology

Cite this

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title = "Proinflammatory Effects of Calprotectin in Graves’ Orbitopathy",
abstract = "Purpose: Early detection and control of inflammation are important to manage Graves’ orbitopathy (GO). We investigated the effects of calprotectin (S100A8/A9) on orbital fibroblast inflammation and GO pathogenesis. Methods: We measured serum calprotectin, S100A8 and S100A9 mRNA expression in orbital fat/connective tissue from GO patients and healthy controls, and proinflammatory cytokines in primary cultured orbital fibroblasts. Results: The serum levels of S100A8/A9 and the expression of S100A8/A9 mRNA in orbital tissue were higher in the GO patients than in the healthy controls. The serum calprotectin levels positively correlated with the clinical activity score and serum thyroid-stimulating immunoglobulin levels. In cultured GO orbital fibroblasts, S100A8/A9 increased the expression of interleukin (IL)-6, IL-8, and monocyte chemotactic protein-1, as well as the phosphorylation of extracellular signal-regulated kinase and nuclear factor-κB. Conclusion: We demonstrated the potential of calprotectin as a biomarker of GO severity and proinflammatory responses to S100A8/A9 in GO orbital fibroblasts.",
author = "Kim, {Ji Won} and Ko, {Jae Sang} and Kim, {Jin Joo} and Jinsook Yoon",
year = "2018",
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doi = "10.1080/09273948.2018.1547835",
language = "English",
journal = "Ocular Immunology and Inflammation",
issn = "0927-3948",
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}

Proinflammatory Effects of Calprotectin in Graves’ Orbitopathy. / Kim, Ji Won; Ko, Jae Sang; Kim, Jin Joo; Yoon, Jinsook.

In: Ocular Immunology and Inflammation, 01.01.2018.

Research output: Contribution to journalArticle

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AU - Kim, Ji Won

AU - Ko, Jae Sang

AU - Kim, Jin Joo

AU - Yoon, Jinsook

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N2 - Purpose: Early detection and control of inflammation are important to manage Graves’ orbitopathy (GO). We investigated the effects of calprotectin (S100A8/A9) on orbital fibroblast inflammation and GO pathogenesis. Methods: We measured serum calprotectin, S100A8 and S100A9 mRNA expression in orbital fat/connective tissue from GO patients and healthy controls, and proinflammatory cytokines in primary cultured orbital fibroblasts. Results: The serum levels of S100A8/A9 and the expression of S100A8/A9 mRNA in orbital tissue were higher in the GO patients than in the healthy controls. The serum calprotectin levels positively correlated with the clinical activity score and serum thyroid-stimulating immunoglobulin levels. In cultured GO orbital fibroblasts, S100A8/A9 increased the expression of interleukin (IL)-6, IL-8, and monocyte chemotactic protein-1, as well as the phosphorylation of extracellular signal-regulated kinase and nuclear factor-κB. Conclusion: We demonstrated the potential of calprotectin as a biomarker of GO severity and proinflammatory responses to S100A8/A9 in GO orbital fibroblasts.

AB - Purpose: Early detection and control of inflammation are important to manage Graves’ orbitopathy (GO). We investigated the effects of calprotectin (S100A8/A9) on orbital fibroblast inflammation and GO pathogenesis. Methods: We measured serum calprotectin, S100A8 and S100A9 mRNA expression in orbital fat/connective tissue from GO patients and healthy controls, and proinflammatory cytokines in primary cultured orbital fibroblasts. Results: The serum levels of S100A8/A9 and the expression of S100A8/A9 mRNA in orbital tissue were higher in the GO patients than in the healthy controls. The serum calprotectin levels positively correlated with the clinical activity score and serum thyroid-stimulating immunoglobulin levels. In cultured GO orbital fibroblasts, S100A8/A9 increased the expression of interleukin (IL)-6, IL-8, and monocyte chemotactic protein-1, as well as the phosphorylation of extracellular signal-regulated kinase and nuclear factor-κB. Conclusion: We demonstrated the potential of calprotectin as a biomarker of GO severity and proinflammatory responses to S100A8/A9 in GO orbital fibroblasts.

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