Proliferation, differentiation, and calcification of preosteoblast-like MC3T3-E1 cells cultured onto noncryrstalline calcium phosphate glass

Yong Keun Lee, Jin Song, Sang Bae Lee, Kwangmahn Kim, Seongho Choi, Chong Kwan Kim, Racquel Z. LeGeros, Kyoung Nam Kim

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The purpose of this study was to investigate calcium phosphate glass as a potential biomaterial for hard tissue repair. We prepared calcium phosphate glass using the system CaO-CaF2-P2O5-MgO-ZnO and cultured MC3T3-E1 cells onto the glass in α-MEM with β-glycerophosphatase and ascorbic acid. Proliferation of the cells was determined to evaluate the biocompatibility of the prepared calcium phosphate glass. The alkaline phosphatase activity was measured to examine the osteoblast differentiation. Mineralization was evaluated by staining the calcium precipitates with Alizarin red. Culture onto the calcium phosphate glass exhibited no significant influence on cell proliferation compared to the polystyrene chosen as a control in this experiment (p > 0.05). The alkaline phosphatase activity in the experimental group, however, was enhanced by the calcium phosphate glass significantly at 10-18 days after incubation than that of the control group (p < 0.05). The promotion of bone-like tissue formation by the calcium phosphate glass was observed after 7 days and thereafter. The results of the present study indicate that the prepared calcium phosphate glass affects osteogenesis by increasing calcification of the extracellular matrix.

Original languageEnglish
Pages (from-to)188-195
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume69
Issue number1
DOIs
Publication statusPublished - 2004 Apr 1

Fingerprint

Calcium phosphate
Glass
phosphorus pentoxide
Phosphatases
Alkaline Phosphatase
Alizarin
Tissue
calcium phosphate
Ascorbic acid
Polystyrenes
Osteoblasts
Cell proliferation
Biocompatible Materials
Biocompatibility
Biomaterials
Ascorbic Acid
Precipitates
Calcium
Bone
Repair

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

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title = "Proliferation, differentiation, and calcification of preosteoblast-like MC3T3-E1 cells cultured onto noncryrstalline calcium phosphate glass",
abstract = "The purpose of this study was to investigate calcium phosphate glass as a potential biomaterial for hard tissue repair. We prepared calcium phosphate glass using the system CaO-CaF2-P2O5-MgO-ZnO and cultured MC3T3-E1 cells onto the glass in α-MEM with β-glycerophosphatase and ascorbic acid. Proliferation of the cells was determined to evaluate the biocompatibility of the prepared calcium phosphate glass. The alkaline phosphatase activity was measured to examine the osteoblast differentiation. Mineralization was evaluated by staining the calcium precipitates with Alizarin red. Culture onto the calcium phosphate glass exhibited no significant influence on cell proliferation compared to the polystyrene chosen as a control in this experiment (p > 0.05). The alkaline phosphatase activity in the experimental group, however, was enhanced by the calcium phosphate glass significantly at 10-18 days after incubation than that of the control group (p < 0.05). The promotion of bone-like tissue formation by the calcium phosphate glass was observed after 7 days and thereafter. The results of the present study indicate that the prepared calcium phosphate glass affects osteogenesis by increasing calcification of the extracellular matrix.",
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Proliferation, differentiation, and calcification of preosteoblast-like MC3T3-E1 cells cultured onto noncryrstalline calcium phosphate glass. / Lee, Yong Keun; Song, Jin; Lee, Sang Bae; Kim, Kwangmahn; Choi, Seongho; Kim, Chong Kwan; LeGeros, Racquel Z.; Kim, Kyoung Nam.

In: Journal of Biomedical Materials Research - Part A, Vol. 69, No. 1, 01.04.2004, p. 188-195.

Research output: Contribution to journalArticle

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AU - LeGeros, Racquel Z.

AU - Kim, Kyoung Nam

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N2 - The purpose of this study was to investigate calcium phosphate glass as a potential biomaterial for hard tissue repair. We prepared calcium phosphate glass using the system CaO-CaF2-P2O5-MgO-ZnO and cultured MC3T3-E1 cells onto the glass in α-MEM with β-glycerophosphatase and ascorbic acid. Proliferation of the cells was determined to evaluate the biocompatibility of the prepared calcium phosphate glass. The alkaline phosphatase activity was measured to examine the osteoblast differentiation. Mineralization was evaluated by staining the calcium precipitates with Alizarin red. Culture onto the calcium phosphate glass exhibited no significant influence on cell proliferation compared to the polystyrene chosen as a control in this experiment (p > 0.05). The alkaline phosphatase activity in the experimental group, however, was enhanced by the calcium phosphate glass significantly at 10-18 days after incubation than that of the control group (p < 0.05). The promotion of bone-like tissue formation by the calcium phosphate glass was observed after 7 days and thereafter. The results of the present study indicate that the prepared calcium phosphate glass affects osteogenesis by increasing calcification of the extracellular matrix.

AB - The purpose of this study was to investigate calcium phosphate glass as a potential biomaterial for hard tissue repair. We prepared calcium phosphate glass using the system CaO-CaF2-P2O5-MgO-ZnO and cultured MC3T3-E1 cells onto the glass in α-MEM with β-glycerophosphatase and ascorbic acid. Proliferation of the cells was determined to evaluate the biocompatibility of the prepared calcium phosphate glass. The alkaline phosphatase activity was measured to examine the osteoblast differentiation. Mineralization was evaluated by staining the calcium precipitates with Alizarin red. Culture onto the calcium phosphate glass exhibited no significant influence on cell proliferation compared to the polystyrene chosen as a control in this experiment (p > 0.05). The alkaline phosphatase activity in the experimental group, however, was enhanced by the calcium phosphate glass significantly at 10-18 days after incubation than that of the control group (p < 0.05). The promotion of bone-like tissue formation by the calcium phosphate glass was observed after 7 days and thereafter. The results of the present study indicate that the prepared calcium phosphate glass affects osteogenesis by increasing calcification of the extracellular matrix.

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