Abstract
Background/Aim: The mechanism responsible for B-cell translocation gene 1 (BTG1) down-regulation in breast carcinoma remains unknown. We examined the BTG1 expression status in breast carcinoma cells and investigated the mechanism underlying the observed alterations. Materials and Methods: Four breast carcinoma cell lines (SK-BR-3, MDA-MB-231, T-47D, and MCF-7), and one normal mammary epithelial cell line (MCF-10A) were analyzed. BTG1 expression was examined using quantitative reverse transcription polymerase chain reaction (PCR) and western blot. Methylation status of the BTG1 promoter was analyzed using methylation-specific PCR (MSP). To investigate the effect of methylation on BTG1, the cells were treated with a demethylating agent. Results: The carcinoma cells expressed significantly lower levels of BTG1 mRNA and protein than normal cells. The BTG1 promoter was highly methylated in the carcinoma cells. 5-aza-2-deoxycytidine significantly restored BTG1 expression. Conclusion: Down-regulation of BTG1 expression through epigenetic repression is involved in mammary carcinogenesis. BTG1 is a potential diagnostic marker and therapeutic target for breast carcinoma.
| Original language | English |
|---|---|
| Pages (from-to) | 5361-5367 |
| Number of pages | 7 |
| Journal | Anticancer research |
| Volume | 39 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2019 |
Bibliographical note
Funding Information:This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Korea government (MSIT) (2018R1D1A1B07047640 and 2018R1C1B5043725).
Publisher Copyright:
© 2019 International Institute of Anticancer Research. All rights reserved.
All Science Journal Classification (ASJC) codes
- Oncology
- Cancer Research
