Promotion of direct angiogenesis in vitro and in vivo by Puerariae flos extract via activation of MEK/ERK-, PI3K/Akt/eNOS-, and Src/FAK-dependent pathways

Byung Hee Chung, Young Lai Cho, Jong Dai Kim, Heui Sug Jo, Moo Ho Won, Hansoo Lee, Kwon Soo Ha, Young-Guen Kwon, Young Myeong Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Puerariae flos has been used for oriental herbal medicine; however, its angiogenic effect has not been elucidated. We found that the extract from Puerariae flos (PFE) increased in vitro angiogenic events, such as endothelial cell proliferation, migration, and tube formation, as well as in vivo neovascularization. These events were followed by the activation of multiple signal modulators, such as extracellular signal-regulated kinase (ERK), Akt, endothelial nitric oxide synthase (eNOS), nitric oxide production, p38, Src, and focal adhesion kinase (FAK), without increasing vascular endothelial growth factor (VEGF) expression. Inhibition of ERK, Akt, and eNOS suppressed PFE-induced angiogenic events, and inhibition of p38 and Src activities blocked PFE-induced endothelial cell migration. PFE did not affect the expression of intracellular adhesion molecule-1 and vascular cell adhesion molecule-1 and transendothelial permeability, which are involved in the adverse effects of the well-known angiogenic inducer VEGF. These results suggest that PFE directly stimulates angiogenesis through the activation of MEK/ERK-, phosphatidylinositol 3-kinase/Akt/eNOS-, and Src/FAK-dependent pathways, without altering VEGF expression, vascular inflammation, and permeability in vitro and in vivo and may be used as a therapeutic agent for ischemic disease and tissue regeneration.

Original languageEnglish
Pages (from-to)934-940
Number of pages7
JournalPhytotherapy Research
Volume24
Issue number6
DOIs
Publication statusPublished - 2010 Jun 1

Fingerprint

Focal Adhesion Protein-Tyrosine Kinases
Nitric Oxide Synthase Type III
Mitogen-Activated Protein Kinase Kinases
Extracellular Signal-Regulated MAP Kinases
Phosphatidylinositol 3-Kinases
Vascular Endothelial Growth Factor A
Cell Movement
Endothelial Cells
Pueraria
East Asian Traditional Medicine
Phosphatidylinositol 3-Kinase
Vascular Cell Adhesion Molecule-1
Herbal Medicine
Capillary Permeability
Regeneration
Permeability
Nitric Oxide
Cell Proliferation
Inflammation
Puerariae Flos plant extract

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Chung, Byung Hee ; Cho, Young Lai ; Kim, Jong Dai ; Jo, Heui Sug ; Won, Moo Ho ; Lee, Hansoo ; Ha, Kwon Soo ; Kwon, Young-Guen ; Kim, Young Myeong. / Promotion of direct angiogenesis in vitro and in vivo by Puerariae flos extract via activation of MEK/ERK-, PI3K/Akt/eNOS-, and Src/FAK-dependent pathways. In: Phytotherapy Research. 2010 ; Vol. 24, No. 6. pp. 934-940.
@article{4e30807d9b6646e286078409a734dda3,
title = "Promotion of direct angiogenesis in vitro and in vivo by Puerariae flos extract via activation of MEK/ERK-, PI3K/Akt/eNOS-, and Src/FAK-dependent pathways",
abstract = "Puerariae flos has been used for oriental herbal medicine; however, its angiogenic effect has not been elucidated. We found that the extract from Puerariae flos (PFE) increased in vitro angiogenic events, such as endothelial cell proliferation, migration, and tube formation, as well as in vivo neovascularization. These events were followed by the activation of multiple signal modulators, such as extracellular signal-regulated kinase (ERK), Akt, endothelial nitric oxide synthase (eNOS), nitric oxide production, p38, Src, and focal adhesion kinase (FAK), without increasing vascular endothelial growth factor (VEGF) expression. Inhibition of ERK, Akt, and eNOS suppressed PFE-induced angiogenic events, and inhibition of p38 and Src activities blocked PFE-induced endothelial cell migration. PFE did not affect the expression of intracellular adhesion molecule-1 and vascular cell adhesion molecule-1 and transendothelial permeability, which are involved in the adverse effects of the well-known angiogenic inducer VEGF. These results suggest that PFE directly stimulates angiogenesis through the activation of MEK/ERK-, phosphatidylinositol 3-kinase/Akt/eNOS-, and Src/FAK-dependent pathways, without altering VEGF expression, vascular inflammation, and permeability in vitro and in vivo and may be used as a therapeutic agent for ischemic disease and tissue regeneration.",
author = "Chung, {Byung Hee} and Cho, {Young Lai} and Kim, {Jong Dai} and Jo, {Heui Sug} and Won, {Moo Ho} and Hansoo Lee and Ha, {Kwon Soo} and Young-Guen Kwon and Kim, {Young Myeong}",
year = "2010",
month = "6",
day = "1",
doi = "10.1002/ptr.3063",
language = "English",
volume = "24",
pages = "934--940",
journal = "Phytotherapy Research",
issn = "0951-418X",
publisher = "John Wiley and Sons Ltd",
number = "6",

}

Promotion of direct angiogenesis in vitro and in vivo by Puerariae flos extract via activation of MEK/ERK-, PI3K/Akt/eNOS-, and Src/FAK-dependent pathways. / Chung, Byung Hee; Cho, Young Lai; Kim, Jong Dai; Jo, Heui Sug; Won, Moo Ho; Lee, Hansoo; Ha, Kwon Soo; Kwon, Young-Guen; Kim, Young Myeong.

In: Phytotherapy Research, Vol. 24, No. 6, 01.06.2010, p. 934-940.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Promotion of direct angiogenesis in vitro and in vivo by Puerariae flos extract via activation of MEK/ERK-, PI3K/Akt/eNOS-, and Src/FAK-dependent pathways

AU - Chung, Byung Hee

AU - Cho, Young Lai

AU - Kim, Jong Dai

AU - Jo, Heui Sug

AU - Won, Moo Ho

AU - Lee, Hansoo

AU - Ha, Kwon Soo

AU - Kwon, Young-Guen

AU - Kim, Young Myeong

PY - 2010/6/1

Y1 - 2010/6/1

N2 - Puerariae flos has been used for oriental herbal medicine; however, its angiogenic effect has not been elucidated. We found that the extract from Puerariae flos (PFE) increased in vitro angiogenic events, such as endothelial cell proliferation, migration, and tube formation, as well as in vivo neovascularization. These events were followed by the activation of multiple signal modulators, such as extracellular signal-regulated kinase (ERK), Akt, endothelial nitric oxide synthase (eNOS), nitric oxide production, p38, Src, and focal adhesion kinase (FAK), without increasing vascular endothelial growth factor (VEGF) expression. Inhibition of ERK, Akt, and eNOS suppressed PFE-induced angiogenic events, and inhibition of p38 and Src activities blocked PFE-induced endothelial cell migration. PFE did not affect the expression of intracellular adhesion molecule-1 and vascular cell adhesion molecule-1 and transendothelial permeability, which are involved in the adverse effects of the well-known angiogenic inducer VEGF. These results suggest that PFE directly stimulates angiogenesis through the activation of MEK/ERK-, phosphatidylinositol 3-kinase/Akt/eNOS-, and Src/FAK-dependent pathways, without altering VEGF expression, vascular inflammation, and permeability in vitro and in vivo and may be used as a therapeutic agent for ischemic disease and tissue regeneration.

AB - Puerariae flos has been used for oriental herbal medicine; however, its angiogenic effect has not been elucidated. We found that the extract from Puerariae flos (PFE) increased in vitro angiogenic events, such as endothelial cell proliferation, migration, and tube formation, as well as in vivo neovascularization. These events were followed by the activation of multiple signal modulators, such as extracellular signal-regulated kinase (ERK), Akt, endothelial nitric oxide synthase (eNOS), nitric oxide production, p38, Src, and focal adhesion kinase (FAK), without increasing vascular endothelial growth factor (VEGF) expression. Inhibition of ERK, Akt, and eNOS suppressed PFE-induced angiogenic events, and inhibition of p38 and Src activities blocked PFE-induced endothelial cell migration. PFE did not affect the expression of intracellular adhesion molecule-1 and vascular cell adhesion molecule-1 and transendothelial permeability, which are involved in the adverse effects of the well-known angiogenic inducer VEGF. These results suggest that PFE directly stimulates angiogenesis through the activation of MEK/ERK-, phosphatidylinositol 3-kinase/Akt/eNOS-, and Src/FAK-dependent pathways, without altering VEGF expression, vascular inflammation, and permeability in vitro and in vivo and may be used as a therapeutic agent for ischemic disease and tissue regeneration.

UR - http://www.scopus.com/inward/record.url?scp=77953280423&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953280423&partnerID=8YFLogxK

U2 - 10.1002/ptr.3063

DO - 10.1002/ptr.3063

M3 - Article

VL - 24

SP - 934

EP - 940

JO - Phytotherapy Research

JF - Phytotherapy Research

SN - 0951-418X

IS - 6

ER -