Prosthecobacter algae sp. nov., isolated from activated sludge using algal metabolites

Jangho Lee, Banghyo Park, Sung Geun Woo, Juyoun Lee, Joonhong Park

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A Gram-stain-negative, fusiform-shaped, facultatively anaerobic bacterial strain, designated EBTL04T, was isolated from activated sludge using algal metabolites and taxonomically characterized through polyphasic investigation. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain EBTL04T belongs to the family Verrucomicrobiaceae, class Verrucomicrobiae, and is closely related to Prosthecobacter dejongeii DSM 12251T (98.6% sequence similarity), Prosthecobacter fusiformis ATCC 25309T (97.9 %), Prosthecobacter debontii DSM 14044T (97.5 %), Prosthecobacter vanneervenii DSM 12252T (94.7 %) and Prosthecobacter fluviatilis KCTC 22182T (93.7 %). The G+C content of the genomic DNA of strain EBTL04T was 62.7 mol%. The menaquinone MK-6 was detected as the predominant quinone. Strain EBTL04T contained phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine as major polar lipids. A fatty acid profile with C16: 1ω5c, iso-C14: 0, C16: 0, anteiso- C15: 0 and C14: 0 as the major components supported the classification of strain EBTL04T in the genus Prosthecobacter. Based on several phenotypic, genotypic and chemotaxonomic features, strain EBTL04T was clearly differentiated from its phylogenetic neighbours. Therefore, strain EBTL04T should be considered to represent a novel species of the genus Prosthecobacter, for which the name Prosthecobacter algae sp. nov. is proposed. The type strain is EBTL04T (5KCTC 23681T5JCM 18053T).

Original languageEnglish
Pages (from-to)663-667
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume64
Issue numberPART 2
DOIs
Publication statusPublished - 2014 Feb 11

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algae
Prosthecobacter
activated sludge
Sewage
metabolite
alga
metabolites
phylogenetics
Phosphatidylglycerols
new species
Phosphatidylserines
Base Composition
rRNA Genes
Names
genomics
Fatty Acids
fatty acid
lipid
Lipids
DNA

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

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title = "Prosthecobacter algae sp. nov., isolated from activated sludge using algal metabolites",
abstract = "A Gram-stain-negative, fusiform-shaped, facultatively anaerobic bacterial strain, designated EBTL04T, was isolated from activated sludge using algal metabolites and taxonomically characterized through polyphasic investigation. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain EBTL04T belongs to the family Verrucomicrobiaceae, class Verrucomicrobiae, and is closely related to Prosthecobacter dejongeii DSM 12251T (98.6{\%} sequence similarity), Prosthecobacter fusiformis ATCC 25309T (97.9 {\%}), Prosthecobacter debontii DSM 14044T (97.5 {\%}), Prosthecobacter vanneervenii DSM 12252T (94.7 {\%}) and Prosthecobacter fluviatilis KCTC 22182T (93.7 {\%}). The G+C content of the genomic DNA of strain EBTL04T was 62.7 mol{\%}. The menaquinone MK-6 was detected as the predominant quinone. Strain EBTL04T contained phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine as major polar lipids. A fatty acid profile with C16: 1ω5c, iso-C14: 0, C16: 0, anteiso- C15: 0 and C14: 0 as the major components supported the classification of strain EBTL04T in the genus Prosthecobacter. Based on several phenotypic, genotypic and chemotaxonomic features, strain EBTL04T was clearly differentiated from its phylogenetic neighbours. Therefore, strain EBTL04T should be considered to represent a novel species of the genus Prosthecobacter, for which the name Prosthecobacter algae sp. nov. is proposed. The type strain is EBTL04T (5KCTC 23681T5JCM 18053T).",
author = "Jangho Lee and Banghyo Park and Woo, {Sung Geun} and Juyoun Lee and Joonhong Park",
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Prosthecobacter algae sp. nov., isolated from activated sludge using algal metabolites. / Lee, Jangho; Park, Banghyo; Woo, Sung Geun; Lee, Juyoun; Park, Joonhong.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 64, No. PART 2, 11.02.2014, p. 663-667.

Research output: Contribution to journalArticle

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AB - A Gram-stain-negative, fusiform-shaped, facultatively anaerobic bacterial strain, designated EBTL04T, was isolated from activated sludge using algal metabolites and taxonomically characterized through polyphasic investigation. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain EBTL04T belongs to the family Verrucomicrobiaceae, class Verrucomicrobiae, and is closely related to Prosthecobacter dejongeii DSM 12251T (98.6% sequence similarity), Prosthecobacter fusiformis ATCC 25309T (97.9 %), Prosthecobacter debontii DSM 14044T (97.5 %), Prosthecobacter vanneervenii DSM 12252T (94.7 %) and Prosthecobacter fluviatilis KCTC 22182T (93.7 %). The G+C content of the genomic DNA of strain EBTL04T was 62.7 mol%. The menaquinone MK-6 was detected as the predominant quinone. Strain EBTL04T contained phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine as major polar lipids. A fatty acid profile with C16: 1ω5c, iso-C14: 0, C16: 0, anteiso- C15: 0 and C14: 0 as the major components supported the classification of strain EBTL04T in the genus Prosthecobacter. Based on several phenotypic, genotypic and chemotaxonomic features, strain EBTL04T was clearly differentiated from its phylogenetic neighbours. Therefore, strain EBTL04T should be considered to represent a novel species of the genus Prosthecobacter, for which the name Prosthecobacter algae sp. nov. is proposed. The type strain is EBTL04T (5KCTC 23681T5JCM 18053T).

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