Protease-activated receptor 2-dependent fluid secretion from airway submucosal glands by house dust mite extract

Hyung Ju Cho, Hyun Jae Lee, Sang Cheol Kim, Kyubo Kim, Yoo Suk Kim, Chang-Hoon Kim, Jeung Gweon Lee, Joo Heon Yoon, Jae Young Choi

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21 Citations (Scopus)

Abstract

Background: The submucosal gland (SMG) is important in the control of airway surface fluid. Protease-activated receptor (PAR) 2 contributes to the pathophysiology of allergies in response to nonspecific allergens bearing proteases and anion secretion. House dust mites (HDMs) have abundant proteases that can activate PAR2, but little is known about the direct effect of HDM on SMG secretion. Objective: The aim of this study was to investigate the effect of HDMs on glandular secretion and its mechanism in allergic patients, patients with chronic rhinosinusitis (CRS), or both. Methods: Inferior nasal turbinates were harvested from 55 patients and classified into 4 groups (the control, allergic rhinitis [AR], CRS, and AR+CRS groups). A microscope attached to a digital camera was used to quantify mucus bubbles from individual SMGs while stimulated with HDM extract, PAR2-activating peptide, and carbachol. PAR2 expression in the SMG was determined by means of immunostaining with anti-PAR2 mAb. Results: HDM induced a significantly higher secretion rate and number of responding glands in the AR and AR+CRS groups than in the control group. Interestingly, patients in the CRS group, who had no HDM-specific IgE antibody, showed a higher response than the control group, and its response was suppressed by a PAR2-selective antagonist. The responses to PAR2-activating peptide were similar to those to HDM, and their secretion rates positively correlated with HDM responses. PAR2 was highly expressed in all 3 disease groups with immunostaining. Conclusions: HDM allergens can induce glandular secretion in patients with AR, CRS, or both, and PAR2 represents a possible mechanism for nonspecific hyperreactivity in inflammatory airway diseases.

Original languageEnglish
JournalJournal of Allergy and Clinical Immunology
Volume129
Issue number2
DOIs
Publication statusPublished - 2012 Jan 1

Fingerprint

PAR-2 Receptor
Fluids and Secretions
Pyroglyphidae
Control Groups
Peptide Hydrolases
Dermatophagoides Antigens
Turbinates
Airway Management
Carbachol
Mucus
Nose
Allergens
Immunoglobulin E
Anions
Hypersensitivity
Allergic Rhinitis
Antibodies

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Cho, Hyung Ju ; Lee, Hyun Jae ; Kim, Sang Cheol ; Kim, Kyubo ; Kim, Yoo Suk ; Kim, Chang-Hoon ; Lee, Jeung Gweon ; Yoon, Joo Heon ; Choi, Jae Young. / Protease-activated receptor 2-dependent fluid secretion from airway submucosal glands by house dust mite extract. In: Journal of Allergy and Clinical Immunology. 2012 ; Vol. 129, No. 2.
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abstract = "Background: The submucosal gland (SMG) is important in the control of airway surface fluid. Protease-activated receptor (PAR) 2 contributes to the pathophysiology of allergies in response to nonspecific allergens bearing proteases and anion secretion. House dust mites (HDMs) have abundant proteases that can activate PAR2, but little is known about the direct effect of HDM on SMG secretion. Objective: The aim of this study was to investigate the effect of HDMs on glandular secretion and its mechanism in allergic patients, patients with chronic rhinosinusitis (CRS), or both. Methods: Inferior nasal turbinates were harvested from 55 patients and classified into 4 groups (the control, allergic rhinitis [AR], CRS, and AR+CRS groups). A microscope attached to a digital camera was used to quantify mucus bubbles from individual SMGs while stimulated with HDM extract, PAR2-activating peptide, and carbachol. PAR2 expression in the SMG was determined by means of immunostaining with anti-PAR2 mAb. Results: HDM induced a significantly higher secretion rate and number of responding glands in the AR and AR+CRS groups than in the control group. Interestingly, patients in the CRS group, who had no HDM-specific IgE antibody, showed a higher response than the control group, and its response was suppressed by a PAR2-selective antagonist. The responses to PAR2-activating peptide were similar to those to HDM, and their secretion rates positively correlated with HDM responses. PAR2 was highly expressed in all 3 disease groups with immunostaining. Conclusions: HDM allergens can induce glandular secretion in patients with AR, CRS, or both, and PAR2 represents a possible mechanism for nonspecific hyperreactivity in inflammatory airway diseases.",
author = "Cho, {Hyung Ju} and Lee, {Hyun Jae} and Kim, {Sang Cheol} and Kyubo Kim and Kim, {Yoo Suk} and Chang-Hoon Kim and Lee, {Jeung Gweon} and Yoon, {Joo Heon} and Choi, {Jae Young}",
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Protease-activated receptor 2-dependent fluid secretion from airway submucosal glands by house dust mite extract. / Cho, Hyung Ju; Lee, Hyun Jae; Kim, Sang Cheol; Kim, Kyubo; Kim, Yoo Suk; Kim, Chang-Hoon; Lee, Jeung Gweon; Yoon, Joo Heon; Choi, Jae Young.

In: Journal of Allergy and Clinical Immunology, Vol. 129, No. 2, 01.01.2012.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Protease-activated receptor 2-dependent fluid secretion from airway submucosal glands by house dust mite extract

AU - Cho, Hyung Ju

AU - Lee, Hyun Jae

AU - Kim, Sang Cheol

AU - Kim, Kyubo

AU - Kim, Yoo Suk

AU - Kim, Chang-Hoon

AU - Lee, Jeung Gweon

AU - Yoon, Joo Heon

AU - Choi, Jae Young

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Background: The submucosal gland (SMG) is important in the control of airway surface fluid. Protease-activated receptor (PAR) 2 contributes to the pathophysiology of allergies in response to nonspecific allergens bearing proteases and anion secretion. House dust mites (HDMs) have abundant proteases that can activate PAR2, but little is known about the direct effect of HDM on SMG secretion. Objective: The aim of this study was to investigate the effect of HDMs on glandular secretion and its mechanism in allergic patients, patients with chronic rhinosinusitis (CRS), or both. Methods: Inferior nasal turbinates were harvested from 55 patients and classified into 4 groups (the control, allergic rhinitis [AR], CRS, and AR+CRS groups). A microscope attached to a digital camera was used to quantify mucus bubbles from individual SMGs while stimulated with HDM extract, PAR2-activating peptide, and carbachol. PAR2 expression in the SMG was determined by means of immunostaining with anti-PAR2 mAb. Results: HDM induced a significantly higher secretion rate and number of responding glands in the AR and AR+CRS groups than in the control group. Interestingly, patients in the CRS group, who had no HDM-specific IgE antibody, showed a higher response than the control group, and its response was suppressed by a PAR2-selective antagonist. The responses to PAR2-activating peptide were similar to those to HDM, and their secretion rates positively correlated with HDM responses. PAR2 was highly expressed in all 3 disease groups with immunostaining. Conclusions: HDM allergens can induce glandular secretion in patients with AR, CRS, or both, and PAR2 represents a possible mechanism for nonspecific hyperreactivity in inflammatory airway diseases.

AB - Background: The submucosal gland (SMG) is important in the control of airway surface fluid. Protease-activated receptor (PAR) 2 contributes to the pathophysiology of allergies in response to nonspecific allergens bearing proteases and anion secretion. House dust mites (HDMs) have abundant proteases that can activate PAR2, but little is known about the direct effect of HDM on SMG secretion. Objective: The aim of this study was to investigate the effect of HDMs on glandular secretion and its mechanism in allergic patients, patients with chronic rhinosinusitis (CRS), or both. Methods: Inferior nasal turbinates were harvested from 55 patients and classified into 4 groups (the control, allergic rhinitis [AR], CRS, and AR+CRS groups). A microscope attached to a digital camera was used to quantify mucus bubbles from individual SMGs while stimulated with HDM extract, PAR2-activating peptide, and carbachol. PAR2 expression in the SMG was determined by means of immunostaining with anti-PAR2 mAb. Results: HDM induced a significantly higher secretion rate and number of responding glands in the AR and AR+CRS groups than in the control group. Interestingly, patients in the CRS group, who had no HDM-specific IgE antibody, showed a higher response than the control group, and its response was suppressed by a PAR2-selective antagonist. The responses to PAR2-activating peptide were similar to those to HDM, and their secretion rates positively correlated with HDM responses. PAR2 was highly expressed in all 3 disease groups with immunostaining. Conclusions: HDM allergens can induce glandular secretion in patients with AR, CRS, or both, and PAR2 represents a possible mechanism for nonspecific hyperreactivity in inflammatory airway diseases.

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