Protection of human fibroblasts from reactive oxygen species by green tea polyphenolic compounds

D. W. Han, H. H. Kim, H. J. Son, H. S. Baek, K. Y. Lee, S. H. Hyon, Jongchul Park

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The potential protective roles played by green tea compounds (GTPCs) against reactive oxygen species-induced oxidative stress in cultured fetal human dermal fibroblasts (fHDFs) were investigated according to cell viability measurement methods, such as fluorescence double staining followed by flow cytometry (FCM), MTT assay and crystal violet uptake. Oxidative stress was induced in the fHDFs, either by adding 50 mM H2O2 or by the action of 40 U/L xanthine oxidase (XO) in the presence of xanthine (250 μM). FCM analysis was the most suitable to show that both treatments produced a significant (p < 0.05) reduction in the fHDF viability, attributed to its high sensitivity. On the microscopic observations, the cell death with necrotic morphology was appreciably induced by both treatments. These oxidative stress-induced damages were significantly (p < 0.05) prevented by pre-incubating the fHDFs with 200 μg/ml GTPC for 1 h. These results suggest that GTPC can act as a biological antioxidant in a cell culture experimental model and prevent oxidative stress-induced cytotoxicity in cells.

Original languageEnglish
Pages (from-to)665-668
Number of pages4
JournalKey Engineering Materials
Volume288-289
Publication statusPublished - 2005 Dec 1

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Polyphenolic compounds
Oxidative stress
Fibroblasts
Reactive Oxygen Species
Oxygen
Flow cytometry
Gentian Violet
Xanthine
Xanthine Oxidase
Cell death
Cytotoxicity
Antioxidants
Cell culture
Assays
Fluorescence
Cells
Crystals
Tea

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Han, D. W., Kim, H. H., Son, H. J., Baek, H. S., Lee, K. Y., Hyon, S. H., & Park, J. (2005). Protection of human fibroblasts from reactive oxygen species by green tea polyphenolic compounds. Key Engineering Materials, 288-289, 665-668.
Han, D. W. ; Kim, H. H. ; Son, H. J. ; Baek, H. S. ; Lee, K. Y. ; Hyon, S. H. ; Park, Jongchul. / Protection of human fibroblasts from reactive oxygen species by green tea polyphenolic compounds. In: Key Engineering Materials. 2005 ; Vol. 288-289. pp. 665-668.
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Han, DW, Kim, HH, Son, HJ, Baek, HS, Lee, KY, Hyon, SH & Park, J 2005, 'Protection of human fibroblasts from reactive oxygen species by green tea polyphenolic compounds', Key Engineering Materials, vol. 288-289, pp. 665-668.

Protection of human fibroblasts from reactive oxygen species by green tea polyphenolic compounds. / Han, D. W.; Kim, H. H.; Son, H. J.; Baek, H. S.; Lee, K. Y.; Hyon, S. H.; Park, Jongchul.

In: Key Engineering Materials, Vol. 288-289, 01.12.2005, p. 665-668.

Research output: Contribution to journalArticle

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Han DW, Kim HH, Son HJ, Baek HS, Lee KY, Hyon SH et al. Protection of human fibroblasts from reactive oxygen species by green tea polyphenolic compounds. Key Engineering Materials. 2005 Dec 1;288-289:665-668.