Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol

Dong Wook Han, Hee Kim Hak, Hee Lee Mi, Sook Baek Hyun, Kwon Yong Lee, Suong Hyu Hyon, Jongchul Park

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Green tea polyphenol (GTP) together with dimethylsulphoxide (DMSO) were added to a freezing solution of osteoblastic cells (rat calvarial osteoblasts and human osteosarcoma cells) exposed to repeated freeze/thaw cycles (FTC) to induce oxidative stress. When cells were subjected to 3 FTCs, freezing medium containing 10% (v/v) DMSO and 500 μg GTP ml-1 significantly (p < 0.05) suppressed cell detachment and growth inhibition by over 63% and protected cell morphology. Furthermore, the alkaline phosphatase activity of osteoblastic cells was appreciably maintained after 2 and 3 FTCs in this mixture. Polyphenols may thus be of use as a cell cryopreservant and be advantageous in such fields as cell transplantation and tissue engineering.

Original languageEnglish
Pages (from-to)655-660
Number of pages6
JournalBiotechnology Letters
Volume27
Issue number9
DOIs
Publication statusPublished - 2005 May 1

Fingerprint

Oxidative stress
Cytoprotection
Polyphenols
Tea
Oxidative Stress
Dimethyl Sulfoxide
Freezing
Transplantation (surgical)
Osteoblasts
Phosphatases
Tissue engineering
Alkaline Phosphatase
Rats
Cells
Cell Engineering
Cell Transplantation
Osteosarcoma
Tissue Engineering
Growth

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Han, Dong Wook ; Hak, Hee Kim ; Mi, Hee Lee ; Hyun, Sook Baek ; Lee, Kwon Yong ; Hyon, Suong Hyu ; Park, Jongchul. / Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol. In: Biotechnology Letters. 2005 ; Vol. 27, No. 9. pp. 655-660.
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Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol. / Han, Dong Wook; Hak, Hee Kim; Mi, Hee Lee; Hyun, Sook Baek; Lee, Kwon Yong; Hyon, Suong Hyu; Park, Jongchul.

In: Biotechnology Letters, Vol. 27, No. 9, 01.05.2005, p. 655-660.

Research output: Contribution to journalArticle

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T1 - Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol

AU - Han, Dong Wook

AU - Hak, Hee Kim

AU - Mi, Hee Lee

AU - Hyun, Sook Baek

AU - Lee, Kwon Yong

AU - Hyon, Suong Hyu

AU - Park, Jongchul

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AB - Green tea polyphenol (GTP) together with dimethylsulphoxide (DMSO) were added to a freezing solution of osteoblastic cells (rat calvarial osteoblasts and human osteosarcoma cells) exposed to repeated freeze/thaw cycles (FTC) to induce oxidative stress. When cells were subjected to 3 FTCs, freezing medium containing 10% (v/v) DMSO and 500 μg GTP ml-1 significantly (p < 0.05) suppressed cell detachment and growth inhibition by over 63% and protected cell morphology. Furthermore, the alkaline phosphatase activity of osteoblastic cells was appreciably maintained after 2 and 3 FTCs in this mixture. Polyphenols may thus be of use as a cell cryopreservant and be advantageous in such fields as cell transplantation and tissue engineering.

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