Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol

Dong Wook Han; Hee Kim Hak; Hee Lee Mi; Sook Baek Hyun; Kwon Yong Lee; Suong Hyu Hyon; Jong Chul Park

doi:10.1007/s10529-005-3677-6

Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol

Dong Wook Han, Hee Kim Hak, Hee Lee Mi, Sook Baek Hyun, Kwon Yong Lee, Suong Hyu Hyon, Jong Chul Park

Department of Medical Engineering

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Green tea polyphenol (GTP) together with dimethylsulphoxide (DMSO) were added to a freezing solution of osteoblastic cells (rat calvarial osteoblasts and human osteosarcoma cells) exposed to repeated freeze/thaw cycles (FTC) to induce oxidative stress. When cells were subjected to 3 FTCs, freezing medium containing 10% (v/v) DMSO and 500 μg GTP ml^-1 significantly (p < 0.05) suppressed cell detachment and growth inhibition by over 63% and protected cell morphology. Furthermore, the alkaline phosphatase activity of osteoblastic cells was appreciably maintained after 2 and 3 FTCs in this mixture. Polyphenols may thus be of use as a cell cryopreservant and be advantageous in such fields as cell transplantation and tissue engineering.

Original language	English
Pages (from-to)	655-660
Number of pages	6
Journal	Biotechnology Letters
Volume	27
Issue number	9
DOIs	https://doi.org/10.1007/s10529-005-3677-6
Publication status	Published - 2005 May

Bibliographical note

Funding Information:
This work was supported by the Korean Ministry of Health and Welfare (Grant No. 02-PJ3-PG3-31402-0018).

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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title = "Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol",

abstract = "Green tea polyphenol (GTP) together with dimethylsulphoxide (DMSO) were added to a freezing solution of osteoblastic cells (rat calvarial osteoblasts and human osteosarcoma cells) exposed to repeated freeze/thaw cycles (FTC) to induce oxidative stress. When cells were subjected to 3 FTCs, freezing medium containing 10% (v/v) DMSO and 500 μg GTP ml-1 significantly (p < 0.05) suppressed cell detachment and growth inhibition by over 63% and protected cell morphology. Furthermore, the alkaline phosphatase activity of osteoblastic cells was appreciably maintained after 2 and 3 FTCs in this mixture. Polyphenols may thus be of use as a cell cryopreservant and be advantageous in such fields as cell transplantation and tissue engineering.",

author = "Han, {Dong Wook} and Hak, {Hee Kim} and Mi, {Hee Lee} and Hyun, {Sook Baek} and Lee, {Kwon Yong} and Hyon, {Suong Hyu} and Park, {Jong Chul}",

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doi = "10.1007/s10529-005-3677-6",

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AU - Han, Dong Wook

AU - Hak, Hee Kim

AU - Mi, Hee Lee

AU - Hyun, Sook Baek

AU - Lee, Kwon Yong

AU - Hyon, Suong Hyu

AU - Park, Jong Chul

N1 - Funding Information: This work was supported by the Korean Ministry of Health and Welfare (Grant No. 02-PJ3-PG3-31402-0018).

PY - 2005/5

Y1 - 2005/5

N2 - Green tea polyphenol (GTP) together with dimethylsulphoxide (DMSO) were added to a freezing solution of osteoblastic cells (rat calvarial osteoblasts and human osteosarcoma cells) exposed to repeated freeze/thaw cycles (FTC) to induce oxidative stress. When cells were subjected to 3 FTCs, freezing medium containing 10% (v/v) DMSO and 500 μg GTP ml-1 significantly (p < 0.05) suppressed cell detachment and growth inhibition by over 63% and protected cell morphology. Furthermore, the alkaline phosphatase activity of osteoblastic cells was appreciably maintained after 2 and 3 FTCs in this mixture. Polyphenols may thus be of use as a cell cryopreservant and be advantageous in such fields as cell transplantation and tissue engineering.

AB - Green tea polyphenol (GTP) together with dimethylsulphoxide (DMSO) were added to a freezing solution of osteoblastic cells (rat calvarial osteoblasts and human osteosarcoma cells) exposed to repeated freeze/thaw cycles (FTC) to induce oxidative stress. When cells were subjected to 3 FTCs, freezing medium containing 10% (v/v) DMSO and 500 μg GTP ml-1 significantly (p < 0.05) suppressed cell detachment and growth inhibition by over 63% and protected cell morphology. Furthermore, the alkaline phosphatase activity of osteoblastic cells was appreciably maintained after 2 and 3 FTCs in this mixture. Polyphenols may thus be of use as a cell cryopreservant and be advantageous in such fields as cell transplantation and tissue engineering.

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JF - Biotechnology Letters

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Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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