Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol

Dong Wook Han, Hee Kim Hak, Hee Lee Mi, Sook Baek Hyun, Kwon Yong Lee, Suong Hyu Hyon, Jong Chul Park

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Green tea polyphenol (GTP) together with dimethylsulphoxide (DMSO) were added to a freezing solution of osteoblastic cells (rat calvarial osteoblasts and human osteosarcoma cells) exposed to repeated freeze/thaw cycles (FTC) to induce oxidative stress. When cells were subjected to 3 FTCs, freezing medium containing 10% (v/v) DMSO and 500 μg GTP ml-1 significantly (p < 0.05) suppressed cell detachment and growth inhibition by over 63% and protected cell morphology. Furthermore, the alkaline phosphatase activity of osteoblastic cells was appreciably maintained after 2 and 3 FTCs in this mixture. Polyphenols may thus be of use as a cell cryopreservant and be advantageous in such fields as cell transplantation and tissue engineering.

Original languageEnglish
Pages (from-to)655-660
Number of pages6
JournalBiotechnology Letters
Issue number9
Publication statusPublished - 2005 May 1


All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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