Protection of rabbit kidney from ischemia/reperfusion injury by green tea polyphenol pretreatment

Kyun Rah Dong, Dong Wook Han, Sook Baek Hyun, Suong Hyu Hyon, Yun Park Beyoung, Jong Chul Park

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) have been implicated in the pathogenesis of renal injury after ischemia/reperfusion (I/R). Recently, green tea polyphenols (GTP) have been found to protect the myocardium and liver against I/R injury. Less attention, however, has been paid to the protective effects of GTP with respect to the kidneys. This study was designed to determine whether GTP could protect renal cells from ischemic injury. The rabbits were divided into three groups of equal size: control (sham-operated), I/R + vehicle (normal saline) and I/R + GTP groups. Each group consisted of six rabbits. Animals underwent 30, 60, 90 and 120 min of ischemia, followed by 24 h of reperfusion, respectively. GTP (200 μg/kg) or the vehicle was administered 45 min prior to commencement of I/R. The results demonstrated that GTP administration resulted in a significant (P < 0.05) reduction of renal damage after 90 min of ischemia, as indicated by the decreased levels of creatinine and urea nitrogen in serum. These results were confirmed by histological examinations, which showed that GTP pretreatment inhibited necrosis and sloughing of the proximal tubules induced by I/R. Examinations also showed decreased necrotic areas in the medulla and decreased glomerular collapse in the I/R-injured rabbits. Moreover, the infiltration of CD8+ T cells was considerably decreased in GTP-treated kidneys. The results of this study suggest that GTP can reduce renal injury by preventing the oxidative stress dependent on I/R and may be used in renal transplantation as an antioxidant.

Original languageEnglish
Pages (from-to)1447-1454
Number of pages8
JournalArchives of pharmacal research
Volume30
Issue number11
Publication statusPublished - 2007 Nov 30

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Polyphenols
Tea
Reperfusion Injury
Rabbits
Kidney
Ischemia
Reperfusion
Oxidative stress
T-cells
Wounds and Injuries
Infiltration
Liver
Kidney Transplantation
Urea
Reactive Oxygen Species
Creatinine
Myocardium
Animals
Oxidative Stress
Necrosis

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Drug Discovery
  • Organic Chemistry

Cite this

Dong, K. R., Han, D. W., Hyun, S. B., Hyon, S. H., Beyoung, Y. P., & Park, J. C. (2007). Protection of rabbit kidney from ischemia/reperfusion injury by green tea polyphenol pretreatment. Archives of pharmacal research, 30(11), 1447-1454.
Dong, Kyun Rah ; Han, Dong Wook ; Hyun, Sook Baek ; Hyon, Suong Hyu ; Beyoung, Yun Park ; Park, Jong Chul. / Protection of rabbit kidney from ischemia/reperfusion injury by green tea polyphenol pretreatment. In: Archives of pharmacal research. 2007 ; Vol. 30, No. 11. pp. 1447-1454.
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Dong, KR, Han, DW, Hyun, SB, Hyon, SH, Beyoung, YP & Park, JC 2007, 'Protection of rabbit kidney from ischemia/reperfusion injury by green tea polyphenol pretreatment', Archives of pharmacal research, vol. 30, no. 11, pp. 1447-1454.

Protection of rabbit kidney from ischemia/reperfusion injury by green tea polyphenol pretreatment. / Dong, Kyun Rah; Han, Dong Wook; Hyun, Sook Baek; Hyon, Suong Hyu; Beyoung, Yun Park; Park, Jong Chul.

In: Archives of pharmacal research, Vol. 30, No. 11, 30.11.2007, p. 1447-1454.

Research output: Contribution to journalArticle

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T1 - Protection of rabbit kidney from ischemia/reperfusion injury by green tea polyphenol pretreatment

AU - Dong, Kyun Rah

AU - Han, Dong Wook

AU - Hyun, Sook Baek

AU - Hyon, Suong Hyu

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AU - Park, Jong Chul

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