Protective effect of agmatine on a reperfusion model after transient cerebral ischemia

Temporal evolution on perfusion mr imaging and histopathologic findings

D. J. Kim, Dong Ik Kim, S. K. Lee, S. H. Suh, Y. J. Lee, J. Kim, T. S. Chung, Jongeun Lee

Research output: Contribution to journalArticle

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Abstract

BACKGROUND AND PURPOSE: The goal of thrombolytic therapy in patients with acute ischemic stroke is early recanalization, but this may result in delayed reperfusion injury. The purpose of this study was to evaluate the neuroprotective effect of agmatine in a transient ischemic cat model by using MR perfusion imaging and histopathologic analyses. METHOD: One-hour temporary occlusion of the left middle cerebral artery of cats was performed in the control ischemia group (n = 10), and 100 mg/kg of agmatine was intravenously injected immediately after recanalization in the agmatine-treated group (n = 15). MR imaging was performed at 1, 24, and 48 hours after recanalization, and the perfusion patterns were investigated. Terminal-deoxynucleotidyl transferase mediated nick and end-labeling (TUNEL) and hematoxylin-eosin (H&E) stainings were performed at the corresponding sections. RESULTS: In the control ischemia group, the number of TUNEL-positive cells was significantly increased in the areas with reperfusion hyperemia (P < .05). In the agmatine-treated group, no significant increase in the number of TUNEL-positive cells was noted in the areas of reperfusion hyperemia. The difference in the number of TUNEL-positive cells between the control ischemia and agmatine-treated group in the areas of reperfusion hyperemia was significant (P < .05). The total number of TUNEL-positive cells and the area of severe ischemic neuronal damage on H&E stain were also significantly attenuated in the agmatine-treated cats compared with the control ischemia cats (P < .05). CONCLUSION: Our results suggest that agmatine has neuroprotective effects against reperfusion injury and ischemia.

Original languageEnglish
Pages (from-to)780-785
Number of pages6
JournalAmerican Journal of Neuroradiology
Volume27
Issue number4
Publication statusPublished - 2006 Apr 1

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Agmatine
Perfusion Imaging
Transient Ischemic Attack
Reperfusion
In Situ Nick-End Labeling
Hyperemia
Cats
Ischemia
Neuroprotective Agents
Reperfusion Injury
Control Groups
DNA Nucleotidylexotransferase
Middle Cerebral Artery Infarction
Thrombolytic Therapy
Hematoxylin
Eosine Yellowish-(YS)
Coloring Agents
Perfusion
Stroke
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging
  • Clinical Neurology

Cite this

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title = "Protective effect of agmatine on a reperfusion model after transient cerebral ischemia: Temporal evolution on perfusion mr imaging and histopathologic findings",
abstract = "BACKGROUND AND PURPOSE: The goal of thrombolytic therapy in patients with acute ischemic stroke is early recanalization, but this may result in delayed reperfusion injury. The purpose of this study was to evaluate the neuroprotective effect of agmatine in a transient ischemic cat model by using MR perfusion imaging and histopathologic analyses. METHOD: One-hour temporary occlusion of the left middle cerebral artery of cats was performed in the control ischemia group (n = 10), and 100 mg/kg of agmatine was intravenously injected immediately after recanalization in the agmatine-treated group (n = 15). MR imaging was performed at 1, 24, and 48 hours after recanalization, and the perfusion patterns were investigated. Terminal-deoxynucleotidyl transferase mediated nick and end-labeling (TUNEL) and hematoxylin-eosin (H&E) stainings were performed at the corresponding sections. RESULTS: In the control ischemia group, the number of TUNEL-positive cells was significantly increased in the areas with reperfusion hyperemia (P < .05). In the agmatine-treated group, no significant increase in the number of TUNEL-positive cells was noted in the areas of reperfusion hyperemia. The difference in the number of TUNEL-positive cells between the control ischemia and agmatine-treated group in the areas of reperfusion hyperemia was significant (P < .05). The total number of TUNEL-positive cells and the area of severe ischemic neuronal damage on H&E stain were also significantly attenuated in the agmatine-treated cats compared with the control ischemia cats (P < .05). CONCLUSION: Our results suggest that agmatine has neuroprotective effects against reperfusion injury and ischemia.",
author = "Kim, {D. J.} and Kim, {Dong Ik} and Lee, {S. K.} and Suh, {S. H.} and Lee, {Y. J.} and J. Kim and Chung, {T. S.} and Jongeun Lee",
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Protective effect of agmatine on a reperfusion model after transient cerebral ischemia : Temporal evolution on perfusion mr imaging and histopathologic findings. / Kim, D. J.; Kim, Dong Ik; Lee, S. K.; Suh, S. H.; Lee, Y. J.; Kim, J.; Chung, T. S.; Lee, Jongeun.

In: American Journal of Neuroradiology, Vol. 27, No. 4, 01.04.2006, p. 780-785.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Protective effect of agmatine on a reperfusion model after transient cerebral ischemia

T2 - Temporal evolution on perfusion mr imaging and histopathologic findings

AU - Kim, D. J.

AU - Kim, Dong Ik

AU - Lee, S. K.

AU - Suh, S. H.

AU - Lee, Y. J.

AU - Kim, J.

AU - Chung, T. S.

AU - Lee, Jongeun

PY - 2006/4/1

Y1 - 2006/4/1

N2 - BACKGROUND AND PURPOSE: The goal of thrombolytic therapy in patients with acute ischemic stroke is early recanalization, but this may result in delayed reperfusion injury. The purpose of this study was to evaluate the neuroprotective effect of agmatine in a transient ischemic cat model by using MR perfusion imaging and histopathologic analyses. METHOD: One-hour temporary occlusion of the left middle cerebral artery of cats was performed in the control ischemia group (n = 10), and 100 mg/kg of agmatine was intravenously injected immediately after recanalization in the agmatine-treated group (n = 15). MR imaging was performed at 1, 24, and 48 hours after recanalization, and the perfusion patterns were investigated. Terminal-deoxynucleotidyl transferase mediated nick and end-labeling (TUNEL) and hematoxylin-eosin (H&E) stainings were performed at the corresponding sections. RESULTS: In the control ischemia group, the number of TUNEL-positive cells was significantly increased in the areas with reperfusion hyperemia (P < .05). In the agmatine-treated group, no significant increase in the number of TUNEL-positive cells was noted in the areas of reperfusion hyperemia. The difference in the number of TUNEL-positive cells between the control ischemia and agmatine-treated group in the areas of reperfusion hyperemia was significant (P < .05). The total number of TUNEL-positive cells and the area of severe ischemic neuronal damage on H&E stain were also significantly attenuated in the agmatine-treated cats compared with the control ischemia cats (P < .05). CONCLUSION: Our results suggest that agmatine has neuroprotective effects against reperfusion injury and ischemia.

AB - BACKGROUND AND PURPOSE: The goal of thrombolytic therapy in patients with acute ischemic stroke is early recanalization, but this may result in delayed reperfusion injury. The purpose of this study was to evaluate the neuroprotective effect of agmatine in a transient ischemic cat model by using MR perfusion imaging and histopathologic analyses. METHOD: One-hour temporary occlusion of the left middle cerebral artery of cats was performed in the control ischemia group (n = 10), and 100 mg/kg of agmatine was intravenously injected immediately after recanalization in the agmatine-treated group (n = 15). MR imaging was performed at 1, 24, and 48 hours after recanalization, and the perfusion patterns were investigated. Terminal-deoxynucleotidyl transferase mediated nick and end-labeling (TUNEL) and hematoxylin-eosin (H&E) stainings were performed at the corresponding sections. RESULTS: In the control ischemia group, the number of TUNEL-positive cells was significantly increased in the areas with reperfusion hyperemia (P < .05). In the agmatine-treated group, no significant increase in the number of TUNEL-positive cells was noted in the areas of reperfusion hyperemia. The difference in the number of TUNEL-positive cells between the control ischemia and agmatine-treated group in the areas of reperfusion hyperemia was significant (P < .05). The total number of TUNEL-positive cells and the area of severe ischemic neuronal damage on H&E stain were also significantly attenuated in the agmatine-treated cats compared with the control ischemia cats (P < .05). CONCLUSION: Our results suggest that agmatine has neuroprotective effects against reperfusion injury and ischemia.

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