Protective effect of lactobacillus fermentum LA12 in an alcohol-induced rat model of alcoholic steatohepatitis

Byoung Kook Kim, In Ock Lee, Pei Lei Tan, Ju Young Eor, Jae-Kwan Hwang, Sae Hun Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Alcoholic liver disease (ALD) is a complex multifaceted disease that involves oxidative stress and inflammation as the key mediators. Despite decades of intensive research, there are no FDA-approved therapies, and/or no effective cure is yet available. Probiotics have received increasing attention in the past few years due to their well-documented gastrointestinal healthpromoting effects. Interestingly, emerging studies have suggested that certain probiotics may offer benefits beyond the gut. Lactobacillus fermentum LA12 has been previously demonstrated to play a role in inflammatory-related disease. However, the possible protective effect of L. fermentum LA12 on ALD still remain to be explored. Thus, the aim of this study was to evaluate the possible protective effect of L. fermentum LA12 on alcohol-induced gut barrier dysfunction and liver damage in a rat model of alcoholic steatohepatitis (ASH). Daily oral administration of L. fermentum LA12 in rat model of ASH for four weeks was shown to significantly reduced intestinal nitric oxide production and hyperpermeability. Moreover, small intestinal histological- and qRT-PCR analysis further revealed that L. fermentum LA12 treatment was capable of up-regulating the mRNA expression levels of tight junction proteins, thereby stimulating the restitution of barrier structure and function. Serum and hepatic analyses also revealed that the restoration of epithelial barrier function may prevent the leakage of endotoxin into the blood, subsequently improve liver function and hepatic steatosis in the L. fermentum LA12-treated rats. Altogether, results in this study suggest that L. fermentum LA12 may be used as a dietary adjunct for the prevention and treatment of ASH.

Original languageEnglish
Pages (from-to)931-939
Number of pages9
JournalKorean Journal for Food Science of Animal Resources
Volume37
Issue number6
DOIs
Publication statusPublished - 2017 Dec 1

Fingerprint

Lactobacillus fermentum
Alcoholic Fatty Liver
fatty liver
protective effect
alcohols
animal models
Alcohols
Alcoholic Liver Diseases
Probiotics
liver diseases
alcohol abuse
probiotics
Liver
digestive system
Tight Junction Proteins
liver
tight junctions
liver function
endotoxins
blood serum

All Science Journal Classification (ASJC) codes

  • Food Science
  • Animal Science and Zoology

Cite this

Kim, Byoung Kook ; Lee, In Ock ; Tan, Pei Lei ; Eor, Ju Young ; Hwang, Jae-Kwan ; Kim, Sae Hun. / Protective effect of lactobacillus fermentum LA12 in an alcohol-induced rat model of alcoholic steatohepatitis. In: Korean Journal for Food Science of Animal Resources. 2017 ; Vol. 37, No. 6. pp. 931-939.
@article{260b20a3d9e84ee0bc9c942b6eeda30a,
title = "Protective effect of lactobacillus fermentum LA12 in an alcohol-induced rat model of alcoholic steatohepatitis",
abstract = "Alcoholic liver disease (ALD) is a complex multifaceted disease that involves oxidative stress and inflammation as the key mediators. Despite decades of intensive research, there are no FDA-approved therapies, and/or no effective cure is yet available. Probiotics have received increasing attention in the past few years due to their well-documented gastrointestinal healthpromoting effects. Interestingly, emerging studies have suggested that certain probiotics may offer benefits beyond the gut. Lactobacillus fermentum LA12 has been previously demonstrated to play a role in inflammatory-related disease. However, the possible protective effect of L. fermentum LA12 on ALD still remain to be explored. Thus, the aim of this study was to evaluate the possible protective effect of L. fermentum LA12 on alcohol-induced gut barrier dysfunction and liver damage in a rat model of alcoholic steatohepatitis (ASH). Daily oral administration of L. fermentum LA12 in rat model of ASH for four weeks was shown to significantly reduced intestinal nitric oxide production and hyperpermeability. Moreover, small intestinal histological- and qRT-PCR analysis further revealed that L. fermentum LA12 treatment was capable of up-regulating the mRNA expression levels of tight junction proteins, thereby stimulating the restitution of barrier structure and function. Serum and hepatic analyses also revealed that the restoration of epithelial barrier function may prevent the leakage of endotoxin into the blood, subsequently improve liver function and hepatic steatosis in the L. fermentum LA12-treated rats. Altogether, results in this study suggest that L. fermentum LA12 may be used as a dietary adjunct for the prevention and treatment of ASH.",
author = "Kim, {Byoung Kook} and Lee, {In Ock} and Tan, {Pei Lei} and Eor, {Ju Young} and Jae-Kwan Hwang and Kim, {Sae Hun}",
year = "2017",
month = "12",
day = "1",
doi = "10.5851/kosfa.2017.37.6.931",
language = "English",
volume = "37",
pages = "931--939",
journal = "Korean Journal for Food Science of Animal Resources",
issn = "1225-8563",
publisher = "Korean Society for Food Science of Animal Resources",
number = "6",

}

Protective effect of lactobacillus fermentum LA12 in an alcohol-induced rat model of alcoholic steatohepatitis. / Kim, Byoung Kook; Lee, In Ock; Tan, Pei Lei; Eor, Ju Young; Hwang, Jae-Kwan; Kim, Sae Hun.

In: Korean Journal for Food Science of Animal Resources, Vol. 37, No. 6, 01.12.2017, p. 931-939.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Protective effect of lactobacillus fermentum LA12 in an alcohol-induced rat model of alcoholic steatohepatitis

AU - Kim, Byoung Kook

AU - Lee, In Ock

AU - Tan, Pei Lei

AU - Eor, Ju Young

AU - Hwang, Jae-Kwan

AU - Kim, Sae Hun

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Alcoholic liver disease (ALD) is a complex multifaceted disease that involves oxidative stress and inflammation as the key mediators. Despite decades of intensive research, there are no FDA-approved therapies, and/or no effective cure is yet available. Probiotics have received increasing attention in the past few years due to their well-documented gastrointestinal healthpromoting effects. Interestingly, emerging studies have suggested that certain probiotics may offer benefits beyond the gut. Lactobacillus fermentum LA12 has been previously demonstrated to play a role in inflammatory-related disease. However, the possible protective effect of L. fermentum LA12 on ALD still remain to be explored. Thus, the aim of this study was to evaluate the possible protective effect of L. fermentum LA12 on alcohol-induced gut barrier dysfunction and liver damage in a rat model of alcoholic steatohepatitis (ASH). Daily oral administration of L. fermentum LA12 in rat model of ASH for four weeks was shown to significantly reduced intestinal nitric oxide production and hyperpermeability. Moreover, small intestinal histological- and qRT-PCR analysis further revealed that L. fermentum LA12 treatment was capable of up-regulating the mRNA expression levels of tight junction proteins, thereby stimulating the restitution of barrier structure and function. Serum and hepatic analyses also revealed that the restoration of epithelial barrier function may prevent the leakage of endotoxin into the blood, subsequently improve liver function and hepatic steatosis in the L. fermentum LA12-treated rats. Altogether, results in this study suggest that L. fermentum LA12 may be used as a dietary adjunct for the prevention and treatment of ASH.

AB - Alcoholic liver disease (ALD) is a complex multifaceted disease that involves oxidative stress and inflammation as the key mediators. Despite decades of intensive research, there are no FDA-approved therapies, and/or no effective cure is yet available. Probiotics have received increasing attention in the past few years due to their well-documented gastrointestinal healthpromoting effects. Interestingly, emerging studies have suggested that certain probiotics may offer benefits beyond the gut. Lactobacillus fermentum LA12 has been previously demonstrated to play a role in inflammatory-related disease. However, the possible protective effect of L. fermentum LA12 on ALD still remain to be explored. Thus, the aim of this study was to evaluate the possible protective effect of L. fermentum LA12 on alcohol-induced gut barrier dysfunction and liver damage in a rat model of alcoholic steatohepatitis (ASH). Daily oral administration of L. fermentum LA12 in rat model of ASH for four weeks was shown to significantly reduced intestinal nitric oxide production and hyperpermeability. Moreover, small intestinal histological- and qRT-PCR analysis further revealed that L. fermentum LA12 treatment was capable of up-regulating the mRNA expression levels of tight junction proteins, thereby stimulating the restitution of barrier structure and function. Serum and hepatic analyses also revealed that the restoration of epithelial barrier function may prevent the leakage of endotoxin into the blood, subsequently improve liver function and hepatic steatosis in the L. fermentum LA12-treated rats. Altogether, results in this study suggest that L. fermentum LA12 may be used as a dietary adjunct for the prevention and treatment of ASH.

UR - http://www.scopus.com/inward/record.url?scp=85044652022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85044652022&partnerID=8YFLogxK

U2 - 10.5851/kosfa.2017.37.6.931

DO - 10.5851/kosfa.2017.37.6.931

M3 - Article

AN - SCOPUS:85044652022

VL - 37

SP - 931

EP - 939

JO - Korean Journal for Food Science of Animal Resources

JF - Korean Journal for Food Science of Animal Resources

SN - 1225-8563

IS - 6

ER -