TY - JOUR
T1 - Protective effects of epicatechin against the toxic effects of streptozotocin on rat pancreatic islets
T2 - In vivo and in vitro
AU - Kim, Myung Jun
AU - Ryu, Gyeong Ryul
AU - Chung, Ji Sung
AU - Sim, Sang Soo
AU - Min, Do Sik
AU - Rhie, Duck Joo
AU - Yoon, Shin Hee
AU - Hahn, Sang June
AU - Kim, Myung Suk
AU - Jo, Yang Hyeok
PY - 2003/4
Y1 - 2003/4
N2 - Introduction: Green tea catechins have diverse pharmacological effects such as anticarcinogenic and antioxidant activities. Aim: To study the protective effects of green tea (-)-epicatechin (EC) against the toxic effects of streptozotocin (STZ), a selective β cell toxin, on pancreatic islets in vivo and in vitro. Methodology: Rats were randomly divided into four groups: control, EC (30 mg/kg)-treated, STZ (60 mg/kg)-treated, and EC plus STZ (same doses; EC+STZ)-treated rats. EC was administered twice a day for 6 days, and a single injection of STZ was used. In EC+STZ-treated rats, EC was administered 6 hours prior to STZ since posttreatment with EC had no beneficial effects on fully developed diabetes in our unpublished study. Insulin and insulin mRNA were detected by immunohistochemical analysis and in situ hybridization, respectively, and physiologic parameters including blood glucose concentration were measured daily. Following isolation of the islets, insulin release, nitrite levels, and islet morphology were observed in the four groups: control, EC (0.8 mM)-treated, STZ (5 mM)-treated, and EC+STZ (same doses)-treated islets. Results: In EC+STZ-treated rats, hyperglycemia and weight loss were not observed and islet morphology was well preserved compared with STZ-treated rats. Compared with STZ treatment alone, insulin release was increased and nitrite production was decreased in EC+STZ-treated islets. Conclusion: EC appears to be helpful in protecting pancreatic islets against exposure to STZ in both in vivo and in vitro systems.
AB - Introduction: Green tea catechins have diverse pharmacological effects such as anticarcinogenic and antioxidant activities. Aim: To study the protective effects of green tea (-)-epicatechin (EC) against the toxic effects of streptozotocin (STZ), a selective β cell toxin, on pancreatic islets in vivo and in vitro. Methodology: Rats were randomly divided into four groups: control, EC (30 mg/kg)-treated, STZ (60 mg/kg)-treated, and EC plus STZ (same doses; EC+STZ)-treated rats. EC was administered twice a day for 6 days, and a single injection of STZ was used. In EC+STZ-treated rats, EC was administered 6 hours prior to STZ since posttreatment with EC had no beneficial effects on fully developed diabetes in our unpublished study. Insulin and insulin mRNA were detected by immunohistochemical analysis and in situ hybridization, respectively, and physiologic parameters including blood glucose concentration were measured daily. Following isolation of the islets, insulin release, nitrite levels, and islet morphology were observed in the four groups: control, EC (0.8 mM)-treated, STZ (5 mM)-treated, and EC+STZ (same doses)-treated islets. Results: In EC+STZ-treated rats, hyperglycemia and weight loss were not observed and islet morphology was well preserved compared with STZ-treated rats. Compared with STZ treatment alone, insulin release was increased and nitrite production was decreased in EC+STZ-treated islets. Conclusion: EC appears to be helpful in protecting pancreatic islets against exposure to STZ in both in vivo and in vitro systems.
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U2 - 10.1097/00006676-200304000-00014
DO - 10.1097/00006676-200304000-00014
M3 - Article
C2 - 12657957
AN - SCOPUS:12244297093
VL - 26
SP - 292
EP - 299
JO - Pancreas
JF - Pancreas
SN - 0885-3177
IS - 3
ER -
