Protective effects of immunization with proteinase, phospholipase and cell wall proteins of candida albicans in mice candidosis

Woon Seob Shin, Sun Ju Choi, Young Chul Yang, Su Jung Park, Joo Young Park, Soo Ki Kim, Kyoung Ho Lee

Research output: Contribution to journalArticle

Abstract

Background: The opportunistic fungus Candida albicans is a major pathogen especially to immunocompromised patients. Objectives: We examined the protective effect of the active and passive immunizations to evaluate the applicability for the treatment of candidosis in Candida-infected mice model. Methods: Candida cell wall components were obtained by treatment of lyticase, proteinase K, and dithiothreitol. The proteinase was purified from the culture filtrates of C albicans using a series of chromatographic steps consisting of DEAE-Sepharose FF, Sephacryl S-200 HR and size-exclusion high performance liquid chromatography. The phospholipase was purified from the culture supernatant of C albicans with DEAE column chromatography, reverse phase column chromatography, revere phase HPLC and size-exclusion HPLC. Antibodies to cell wall protein components, proteinase and phospholipase were produced by immunization into mice of same strain. Results: The mean survival times of active and passive immunized mice groups were longer than those of non-immunized groups. Conclusion: These results showed that immunization with proteinase and its antibody were the most effective to prolong survival time in Candida-infected mice.

Original languageEnglish
Pages (from-to)9-15
Number of pages7
JournalKorean Journal of Medical Mycology
Volume14
Issue number1
Publication statusPublished - 2009 Mar 1

All Science Journal Classification (ASJC) codes

  • Infectious Diseases

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