Protective effects of panduratin A against oxidative damage of tert-butylhydroperoxide in human HepG2 cells

Jong Hee Sohn, Kyu Lee Han, Sun Hee Lee, Jae Kwan Hwang

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

The protective effect of panduratin A, isolated from Kaempferia pandurata ROXB. (Zingiberaceae), against tert-butylhydroperoxide (t-BHP)-induced cytotoxicity was investigated in a human hepatoma cell line, HepG2. The tetrazolium dye colorimetric test (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay) was used to monitor cytotoxicity. Lipid peroxidation [malondialdehyde (MDA) formation] and intracellular glutathione level were estimated by fluorometric methods. Intracellular reactive oxygen species (ROS) formation was measured using a fluorescent probe 2′,7′-dichlorofluorescein diacetate (DCFH-DA). Panduratin A significantly reduced the cell growth inhibition caused by t-BHP. Furthermore, panduratin A ameliorated lipid peroxidation as demonstrated by a reduction in MDA formation, and attenuated glutathione (GSH) depletion in a dose-dependent manner. It was also found that panduratin A reduced intracellular ROS formation caused by t-BHP. These results strongly suggest that panduratin A has significant protective ability against oxidative damage caused by reactive intermediates.

Original languageEnglish
Pages (from-to)1083-1086
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume28
Issue number6
DOIs
Publication statusPublished - 2005 Jun

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

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