Protective efficacy of crude virus-like particle vaccine against HPAI H5N1 in chickens and its application on DIVA strategy

Jae Keun Park, Dong Hun Lee, Ha Na Youn, Myeong Seob Kim, Yu Na Lee, Seong Su Yuk, Tae Hyun Lim, Jun Hyuk Jang, Jung Hoon Kwon, Byoung Yoon Kim, Sang Moo Kang, Baik Lin Seong, Joong Bok Lee, Seung Yong Park, In Soo Choi, Chang Seon Song

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background Currently, Asian lineage highly pathogenic avian influenza (HPAI) H5N1 has become widespread across continents. These viruses are persistently circulating among poultry populations in endemic regions, causing huge economic losses, and raising concerns about an H5N1 pandemic. To control HPAI H5N1, effective vaccines for poultry are urgently needed. Objective In this study, we developed HPAI virus-like particle (VLP) vaccine as a candidate poultry vaccine and evaluated its protective efficacy and possible application for differentiating infected from vaccinated animals (DIVA). Methods Specific pathogen-free chickens received a single injection of HPAI H5N1 VLP vaccine generated using baculovirus expression vector system. Immunogenicity of VLP vaccines was determined using hemagglutination inhibition (HI), neuraminidase inhibition (NI), and ELISA test. Challenge study was performed to evaluate efficacy of VLP vaccines. Results and Conclusions A single immunization with HPAI H5N1 VLP vaccine induced high levels of HI and NI antibodies and protected chickens from a lethal challenge of wild-type HPAI H5N1 virus. Viral excretion from the vaccinated and challenged group was strongly reduced compared with a mock-vaccinated control group. Furthermore, we were able to differentiate VLP-vaccinated chickens from vaccinated and then infected chickens with a commercial ELISA test kit, which offers a promising strategy for the application of DIVA concept.

Original languageEnglish
Pages (from-to)340-348
Number of pages9
JournalInfluenza and other Respiratory Viruses
Volume7
Issue number3
DOIs
Publication statusPublished - 2013 May 1

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Virus-Like Particle Vaccines
Influenza in Birds
Chickens
H5N1 Subtype Influenza A Virus
Orthomyxoviridae
Poultry
Hemagglutination
Neuraminidase
Vaccines
Enzyme-Linked Immunosorbent Assay
Specific Pathogen-Free Organisms
Influenza Vaccines
Baculoviridae
Pandemics
Virion
Immunization
Economics
Viruses
Control Groups
Injections

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Pulmonary and Respiratory Medicine
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Park, Jae Keun ; Lee, Dong Hun ; Youn, Ha Na ; Kim, Myeong Seob ; Lee, Yu Na ; Yuk, Seong Su ; Lim, Tae Hyun ; Jang, Jun Hyuk ; Kwon, Jung Hoon ; Kim, Byoung Yoon ; Kang, Sang Moo ; Seong, Baik Lin ; Lee, Joong Bok ; Park, Seung Yong ; Choi, In Soo ; Song, Chang Seon. / Protective efficacy of crude virus-like particle vaccine against HPAI H5N1 in chickens and its application on DIVA strategy. In: Influenza and other Respiratory Viruses. 2013 ; Vol. 7, No. 3. pp. 340-348.
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title = "Protective efficacy of crude virus-like particle vaccine against HPAI H5N1 in chickens and its application on DIVA strategy",
abstract = "Background Currently, Asian lineage highly pathogenic avian influenza (HPAI) H5N1 has become widespread across continents. These viruses are persistently circulating among poultry populations in endemic regions, causing huge economic losses, and raising concerns about an H5N1 pandemic. To control HPAI H5N1, effective vaccines for poultry are urgently needed. Objective In this study, we developed HPAI virus-like particle (VLP) vaccine as a candidate poultry vaccine and evaluated its protective efficacy and possible application for differentiating infected from vaccinated animals (DIVA). Methods Specific pathogen-free chickens received a single injection of HPAI H5N1 VLP vaccine generated using baculovirus expression vector system. Immunogenicity of VLP vaccines was determined using hemagglutination inhibition (HI), neuraminidase inhibition (NI), and ELISA test. Challenge study was performed to evaluate efficacy of VLP vaccines. Results and Conclusions A single immunization with HPAI H5N1 VLP vaccine induced high levels of HI and NI antibodies and protected chickens from a lethal challenge of wild-type HPAI H5N1 virus. Viral excretion from the vaccinated and challenged group was strongly reduced compared with a mock-vaccinated control group. Furthermore, we were able to differentiate VLP-vaccinated chickens from vaccinated and then infected chickens with a commercial ELISA test kit, which offers a promising strategy for the application of DIVA concept.",
author = "Park, {Jae Keun} and Lee, {Dong Hun} and Youn, {Ha Na} and Kim, {Myeong Seob} and Lee, {Yu Na} and Yuk, {Seong Su} and Lim, {Tae Hyun} and Jang, {Jun Hyuk} and Kwon, {Jung Hoon} and Kim, {Byoung Yoon} and Kang, {Sang Moo} and Seong, {Baik Lin} and Lee, {Joong Bok} and Park, {Seung Yong} and Choi, {In Soo} and Song, {Chang Seon}",
year = "2013",
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Park, JK, Lee, DH, Youn, HN, Kim, MS, Lee, YN, Yuk, SS, Lim, TH, Jang, JH, Kwon, JH, Kim, BY, Kang, SM, Seong, BL, Lee, JB, Park, SY, Choi, IS & Song, CS 2013, 'Protective efficacy of crude virus-like particle vaccine against HPAI H5N1 in chickens and its application on DIVA strategy', Influenza and other Respiratory Viruses, vol. 7, no. 3, pp. 340-348. https://doi.org/10.1111/j.1750-2659.2012.00396.x

Protective efficacy of crude virus-like particle vaccine against HPAI H5N1 in chickens and its application on DIVA strategy. / Park, Jae Keun; Lee, Dong Hun; Youn, Ha Na; Kim, Myeong Seob; Lee, Yu Na; Yuk, Seong Su; Lim, Tae Hyun; Jang, Jun Hyuk; Kwon, Jung Hoon; Kim, Byoung Yoon; Kang, Sang Moo; Seong, Baik Lin; Lee, Joong Bok; Park, Seung Yong; Choi, In Soo; Song, Chang Seon.

In: Influenza and other Respiratory Viruses, Vol. 7, No. 3, 01.05.2013, p. 340-348.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Protective efficacy of crude virus-like particle vaccine against HPAI H5N1 in chickens and its application on DIVA strategy

AU - Park, Jae Keun

AU - Lee, Dong Hun

AU - Youn, Ha Na

AU - Kim, Myeong Seob

AU - Lee, Yu Na

AU - Yuk, Seong Su

AU - Lim, Tae Hyun

AU - Jang, Jun Hyuk

AU - Kwon, Jung Hoon

AU - Kim, Byoung Yoon

AU - Kang, Sang Moo

AU - Seong, Baik Lin

AU - Lee, Joong Bok

AU - Park, Seung Yong

AU - Choi, In Soo

AU - Song, Chang Seon

PY - 2013/5/1

Y1 - 2013/5/1

N2 - Background Currently, Asian lineage highly pathogenic avian influenza (HPAI) H5N1 has become widespread across continents. These viruses are persistently circulating among poultry populations in endemic regions, causing huge economic losses, and raising concerns about an H5N1 pandemic. To control HPAI H5N1, effective vaccines for poultry are urgently needed. Objective In this study, we developed HPAI virus-like particle (VLP) vaccine as a candidate poultry vaccine and evaluated its protective efficacy and possible application for differentiating infected from vaccinated animals (DIVA). Methods Specific pathogen-free chickens received a single injection of HPAI H5N1 VLP vaccine generated using baculovirus expression vector system. Immunogenicity of VLP vaccines was determined using hemagglutination inhibition (HI), neuraminidase inhibition (NI), and ELISA test. Challenge study was performed to evaluate efficacy of VLP vaccines. Results and Conclusions A single immunization with HPAI H5N1 VLP vaccine induced high levels of HI and NI antibodies and protected chickens from a lethal challenge of wild-type HPAI H5N1 virus. Viral excretion from the vaccinated and challenged group was strongly reduced compared with a mock-vaccinated control group. Furthermore, we were able to differentiate VLP-vaccinated chickens from vaccinated and then infected chickens with a commercial ELISA test kit, which offers a promising strategy for the application of DIVA concept.

AB - Background Currently, Asian lineage highly pathogenic avian influenza (HPAI) H5N1 has become widespread across continents. These viruses are persistently circulating among poultry populations in endemic regions, causing huge economic losses, and raising concerns about an H5N1 pandemic. To control HPAI H5N1, effective vaccines for poultry are urgently needed. Objective In this study, we developed HPAI virus-like particle (VLP) vaccine as a candidate poultry vaccine and evaluated its protective efficacy and possible application for differentiating infected from vaccinated animals (DIVA). Methods Specific pathogen-free chickens received a single injection of HPAI H5N1 VLP vaccine generated using baculovirus expression vector system. Immunogenicity of VLP vaccines was determined using hemagglutination inhibition (HI), neuraminidase inhibition (NI), and ELISA test. Challenge study was performed to evaluate efficacy of VLP vaccines. Results and Conclusions A single immunization with HPAI H5N1 VLP vaccine induced high levels of HI and NI antibodies and protected chickens from a lethal challenge of wild-type HPAI H5N1 virus. Viral excretion from the vaccinated and challenged group was strongly reduced compared with a mock-vaccinated control group. Furthermore, we were able to differentiate VLP-vaccinated chickens from vaccinated and then infected chickens with a commercial ELISA test kit, which offers a promising strategy for the application of DIVA concept.

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