Protein-conjugated, glucose-sensitive surface using fluorescent dendrimer porphyrin

Yeol Lee, Jongsu Kim, Suhyun Kim, Woo Dong Jang, Sangphil Park, Won-Gun Koh

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A multi-functional dendrimer-coated surface has been prepared for the effective protein immobilization and detection of protein activity. The silicon surface was first modified with positively-charged amino groups using 3-aminopropyltriethoxysilane (APTES), and subsequently coated with ionic dendrimer porphyrin (DP) by electrostatic interaction. Fluorescence and atomic force microscopy (AFM) studies showed that the dendrimer was homogeneously coated on the APTES-modified silicon surface as dome-shaped features that protruded 1.0-2.5 nm above the surface and had diameters ranging from 50 nm to 100 nm. The dendrimer-modified surface showed a higher capacity to covalently bind proteins, compared to the control surfaces, and the protein activity was higher by a factor of two. Using the fluorescent property of the porphyrin core, the relative amounts of dendrimer and the enzyme-catalyzed reactions on the dendrimer-coated surface were examined by fluorescence microscopy. Glucose oxidase (GOX)-mediated glucose oxidation quenched fluorescence emission from the focal porphyrin core through a peroxidase-coupled system and from the quantitative relationship between quenching and glucose concentration, the GOX-catalyzed reaction could be characterized.

Original languageEnglish
Pages (from-to)5643-5647
Number of pages5
JournalJournal of Materials Chemistry
Volume19
Issue number31
DOIs
Publication statusPublished - 2009 Aug 10

Fingerprint

Dendrimers
Porphyrins
Glucose
Proteins
Glucose Oxidase
Glucose oxidase
Silicon
Fluorescence
Control surfaces
Fluorescence microscopy
Domes
Coulomb interactions
Peroxidase
Quenching
Atomic force microscopy
Membrane Proteins
Enzymes
Oxidation

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Chemistry

Cite this

Lee, Yeol ; Kim, Jongsu ; Kim, Suhyun ; Jang, Woo Dong ; Park, Sangphil ; Koh, Won-Gun. / Protein-conjugated, glucose-sensitive surface using fluorescent dendrimer porphyrin. In: Journal of Materials Chemistry. 2009 ; Vol. 19, No. 31. pp. 5643-5647.
@article{d2c700021ec843a287bb30cf53623eab,
title = "Protein-conjugated, glucose-sensitive surface using fluorescent dendrimer porphyrin",
abstract = "A multi-functional dendrimer-coated surface has been prepared for the effective protein immobilization and detection of protein activity. The silicon surface was first modified with positively-charged amino groups using 3-aminopropyltriethoxysilane (APTES), and subsequently coated with ionic dendrimer porphyrin (DP) by electrostatic interaction. Fluorescence and atomic force microscopy (AFM) studies showed that the dendrimer was homogeneously coated on the APTES-modified silicon surface as dome-shaped features that protruded 1.0-2.5 nm above the surface and had diameters ranging from 50 nm to 100 nm. The dendrimer-modified surface showed a higher capacity to covalently bind proteins, compared to the control surfaces, and the protein activity was higher by a factor of two. Using the fluorescent property of the porphyrin core, the relative amounts of dendrimer and the enzyme-catalyzed reactions on the dendrimer-coated surface were examined by fluorescence microscopy. Glucose oxidase (GOX)-mediated glucose oxidation quenched fluorescence emission from the focal porphyrin core through a peroxidase-coupled system and from the quantitative relationship between quenching and glucose concentration, the GOX-catalyzed reaction could be characterized.",
author = "Yeol Lee and Jongsu Kim and Suhyun Kim and Jang, {Woo Dong} and Sangphil Park and Won-Gun Koh",
year = "2009",
month = "8",
day = "10",
doi = "10.1039/b906587n",
language = "English",
volume = "19",
pages = "5643--5647",
journal = "Journal of Materials Chemistry",
issn = "0959-9428",
publisher = "Royal Society of Chemistry",
number = "31",

}

Protein-conjugated, glucose-sensitive surface using fluorescent dendrimer porphyrin. / Lee, Yeol; Kim, Jongsu; Kim, Suhyun; Jang, Woo Dong; Park, Sangphil; Koh, Won-Gun.

In: Journal of Materials Chemistry, Vol. 19, No. 31, 10.08.2009, p. 5643-5647.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Protein-conjugated, glucose-sensitive surface using fluorescent dendrimer porphyrin

AU - Lee, Yeol

AU - Kim, Jongsu

AU - Kim, Suhyun

AU - Jang, Woo Dong

AU - Park, Sangphil

AU - Koh, Won-Gun

PY - 2009/8/10

Y1 - 2009/8/10

N2 - A multi-functional dendrimer-coated surface has been prepared for the effective protein immobilization and detection of protein activity. The silicon surface was first modified with positively-charged amino groups using 3-aminopropyltriethoxysilane (APTES), and subsequently coated with ionic dendrimer porphyrin (DP) by electrostatic interaction. Fluorescence and atomic force microscopy (AFM) studies showed that the dendrimer was homogeneously coated on the APTES-modified silicon surface as dome-shaped features that protruded 1.0-2.5 nm above the surface and had diameters ranging from 50 nm to 100 nm. The dendrimer-modified surface showed a higher capacity to covalently bind proteins, compared to the control surfaces, and the protein activity was higher by a factor of two. Using the fluorescent property of the porphyrin core, the relative amounts of dendrimer and the enzyme-catalyzed reactions on the dendrimer-coated surface were examined by fluorescence microscopy. Glucose oxidase (GOX)-mediated glucose oxidation quenched fluorescence emission from the focal porphyrin core through a peroxidase-coupled system and from the quantitative relationship between quenching and glucose concentration, the GOX-catalyzed reaction could be characterized.

AB - A multi-functional dendrimer-coated surface has been prepared for the effective protein immobilization and detection of protein activity. The silicon surface was first modified with positively-charged amino groups using 3-aminopropyltriethoxysilane (APTES), and subsequently coated with ionic dendrimer porphyrin (DP) by electrostatic interaction. Fluorescence and atomic force microscopy (AFM) studies showed that the dendrimer was homogeneously coated on the APTES-modified silicon surface as dome-shaped features that protruded 1.0-2.5 nm above the surface and had diameters ranging from 50 nm to 100 nm. The dendrimer-modified surface showed a higher capacity to covalently bind proteins, compared to the control surfaces, and the protein activity was higher by a factor of two. Using the fluorescent property of the porphyrin core, the relative amounts of dendrimer and the enzyme-catalyzed reactions on the dendrimer-coated surface were examined by fluorescence microscopy. Glucose oxidase (GOX)-mediated glucose oxidation quenched fluorescence emission from the focal porphyrin core through a peroxidase-coupled system and from the quantitative relationship between quenching and glucose concentration, the GOX-catalyzed reaction could be characterized.

UR - http://www.scopus.com/inward/record.url?scp=68149172906&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=68149172906&partnerID=8YFLogxK

U2 - 10.1039/b906587n

DO - 10.1039/b906587n

M3 - Article

VL - 19

SP - 5643

EP - 5647

JO - Journal of Materials Chemistry

JF - Journal of Materials Chemistry

SN - 0959-9428

IS - 31

ER -