Protein-Engineered Large Area Adipose-derived Stem Cell Sheets for Wound Healing

Jongbeom Na, Seung Yong Song, Jae Dong Kim, Minsu Han, June Seok Heo, Chae Eun Yang, Hyun Ok Kim, Dae Hyun Lew, Eunkyoung Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Human adipose-derived stem cells (hADSCs) formed robust cell sheets by engineering the cells with soluble cell adhesive molecules (CAMs), which enabled unique approaches to harvest large area hADSC sheets. As a soluble CAM, fibronectin (FN) (100 pg/ml) enhanced the cell proliferation rate and control both cell-to-cell and cell-to-substrate interactions. Through this engineering of FN, a transferrable hADSC sheet was obtained as a free-stranding sheet (122.6 mm2) by a photothermal method. During the harvesting of hADSC sheets by the photothermal method, a collagen layer in-between cells and conductive polymer film (CP) was dissociated, to protect cells from direct exposure to a near infrared (NIR) source. The hADSC sheets were applied to chronic wound of genetically diabetic db/db mice in vivo, to accelerate 30% faster wound closure with a high closure effect (εwc) than that of control groups. These results indicated that the engineering of CAM and collagens allow hADSC sheet harvesting, which could be extended to engineer various stem cell sheets for efficient therapies.

Original languageEnglish
Article number15869
JournalScientific reports
Volume8
Issue number1
DOIs
Publication statusPublished - 2018 Dec 1

Fingerprint

Wound Healing
Stem Cells
Adhesives
Proteins
Cell Engineering
Fibronectins
Collagen
Wounds and Injuries
Cell Communication
Polymers
Cell Proliferation
Control Groups

All Science Journal Classification (ASJC) codes

  • General

Cite this

Na, Jongbeom ; Song, Seung Yong ; Kim, Jae Dong ; Han, Minsu ; Heo, June Seok ; Yang, Chae Eun ; Kim, Hyun Ok ; Lew, Dae Hyun ; Kim, Eunkyoung. / Protein-Engineered Large Area Adipose-derived Stem Cell Sheets for Wound Healing. In: Scientific reports. 2018 ; Vol. 8, No. 1.
@article{bde77c8096374209ba828a2c40b4911d,
title = "Protein-Engineered Large Area Adipose-derived Stem Cell Sheets for Wound Healing",
abstract = "Human adipose-derived stem cells (hADSCs) formed robust cell sheets by engineering the cells with soluble cell adhesive molecules (CAMs), which enabled unique approaches to harvest large area hADSC sheets. As a soluble CAM, fibronectin (FN) (100 pg/ml) enhanced the cell proliferation rate and control both cell-to-cell and cell-to-substrate interactions. Through this engineering of FN, a transferrable hADSC sheet was obtained as a free-stranding sheet (122.6 mm2) by a photothermal method. During the harvesting of hADSC sheets by the photothermal method, a collagen layer in-between cells and conductive polymer film (CP) was dissociated, to protect cells from direct exposure to a near infrared (NIR) source. The hADSC sheets were applied to chronic wound of genetically diabetic db/db mice in vivo, to accelerate 30{\%} faster wound closure with a high closure effect (εwc) than that of control groups. These results indicated that the engineering of CAM and collagens allow hADSC sheet harvesting, which could be extended to engineer various stem cell sheets for efficient therapies.",
author = "Jongbeom Na and Song, {Seung Yong} and Kim, {Jae Dong} and Minsu Han and Heo, {June Seok} and Yang, {Chae Eun} and Kim, {Hyun Ok} and Lew, {Dae Hyun} and Eunkyoung Kim",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41598-018-34119-x",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

Na, J, Song, SY, Kim, JD, Han, M, Heo, JS, Yang, CE, Kim, HO, Lew, DH & Kim, E 2018, 'Protein-Engineered Large Area Adipose-derived Stem Cell Sheets for Wound Healing', Scientific reports, vol. 8, no. 1, 15869. https://doi.org/10.1038/s41598-018-34119-x

Protein-Engineered Large Area Adipose-derived Stem Cell Sheets for Wound Healing. / Na, Jongbeom; Song, Seung Yong; Kim, Jae Dong; Han, Minsu; Heo, June Seok; Yang, Chae Eun; Kim, Hyun Ok; Lew, Dae Hyun; Kim, Eunkyoung.

In: Scientific reports, Vol. 8, No. 1, 15869, 01.12.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Protein-Engineered Large Area Adipose-derived Stem Cell Sheets for Wound Healing

AU - Na, Jongbeom

AU - Song, Seung Yong

AU - Kim, Jae Dong

AU - Han, Minsu

AU - Heo, June Seok

AU - Yang, Chae Eun

AU - Kim, Hyun Ok

AU - Lew, Dae Hyun

AU - Kim, Eunkyoung

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Human adipose-derived stem cells (hADSCs) formed robust cell sheets by engineering the cells with soluble cell adhesive molecules (CAMs), which enabled unique approaches to harvest large area hADSC sheets. As a soluble CAM, fibronectin (FN) (100 pg/ml) enhanced the cell proliferation rate and control both cell-to-cell and cell-to-substrate interactions. Through this engineering of FN, a transferrable hADSC sheet was obtained as a free-stranding sheet (122.6 mm2) by a photothermal method. During the harvesting of hADSC sheets by the photothermal method, a collagen layer in-between cells and conductive polymer film (CP) was dissociated, to protect cells from direct exposure to a near infrared (NIR) source. The hADSC sheets were applied to chronic wound of genetically diabetic db/db mice in vivo, to accelerate 30% faster wound closure with a high closure effect (εwc) than that of control groups. These results indicated that the engineering of CAM and collagens allow hADSC sheet harvesting, which could be extended to engineer various stem cell sheets for efficient therapies.

AB - Human adipose-derived stem cells (hADSCs) formed robust cell sheets by engineering the cells with soluble cell adhesive molecules (CAMs), which enabled unique approaches to harvest large area hADSC sheets. As a soluble CAM, fibronectin (FN) (100 pg/ml) enhanced the cell proliferation rate and control both cell-to-cell and cell-to-substrate interactions. Through this engineering of FN, a transferrable hADSC sheet was obtained as a free-stranding sheet (122.6 mm2) by a photothermal method. During the harvesting of hADSC sheets by the photothermal method, a collagen layer in-between cells and conductive polymer film (CP) was dissociated, to protect cells from direct exposure to a near infrared (NIR) source. The hADSC sheets were applied to chronic wound of genetically diabetic db/db mice in vivo, to accelerate 30% faster wound closure with a high closure effect (εwc) than that of control groups. These results indicated that the engineering of CAM and collagens allow hADSC sheet harvesting, which could be extended to engineer various stem cell sheets for efficient therapies.

UR - http://www.scopus.com/inward/record.url?scp=85055618271&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055618271&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-34119-x

DO - 10.1038/s41598-018-34119-x

M3 - Article

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 15869

ER -