Protein profiling of human plasma samples by two-dimensional electrophoresis

Sang Yun Cho, Eun Young Lee, Hye Young Kim, Min Jung Kang, Hyoung Joo Lee, Hoguen Kim, Young Ki Paik

Research output: Chapter in Book/Report/Conference proceedingChapter

16 Citations (Scopus)

Abstract

Human plasma is regarded the most complex and well-known clinical specimen that can be easily obtained; alterations in the levels of plasma proteins or their corresponding enzyme activities may reflect either a healthy or a diseased state. Given that there is no defined genomic information as to the intact protein components in plasma, protein profiling could be the first, step toward its molecular characterization. Several problems exist in the analysis of plasma proteins, however. For example, the widest dynamic range of protein concentrations, the presence of high-abundance proteins, and post-translational modifications need to be considered before proteomic studies are undertaken. In particular, efficient, depletion or pre-fractionation of high-abundance proteins is crucial for the identification of low-abundance proteins that may contain potential biomarkers. After the removal of high-abundance proteins, protein profiling can be initiated using two-dimensional electrophoresis (2DE), which has been widely used for displaying the differential proteome under specific physiological, conditions. Here, we describe a typical 2DE procedure for plasma proteome under either a healthy or a diseased state (e.g., liver cancer) in which pre-fractionation and depletion are integral steps in the search for disease biomarkers.

Original languageEnglish
Title of host publicationClinical Proteomics - Methods and Protocols
Pages57-75
Number of pages19
Publication statusPublished - 2007 Aug 31

Publication series

NameMethods in Molecular Biology
Volume428
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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    Cho, S. Y., Lee, E. Y., Kim, H. Y., Kang, M. J., Lee, H. J., Kim, H., & Paik, Y. K. (2007). Protein profiling of human plasma samples by two-dimensional electrophoresis. In Clinical Proteomics - Methods and Protocols (pp. 57-75). (Methods in Molecular Biology; Vol. 428).