Protein solubility and folding enhancement by interaction with RNA

Seong Il Choi, Kyoung Sim Han, Chul Woo Kim, Ki Sun Ryu, Byung Hee Kim, Kyun Hwan Kim, Seo Il Kim, Tae Hyun Kang, Hang Cheol Shin, Keo Heun Lim, Hyo Kyung Kim, Jeong Min Hyun, Baik Lin Seong

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

While basic mechanisms of several major molecular chaperones are well understood, this machinery has been known to be involved in folding of only limited number of proteins inside the cells. Here, we report a chaperone type of protein folding facilitated by interaction with RNA. When an RNA-binding module is placed at the N-terminus of aggregation-prone target proteins, this module, upon binding with RNA, further promotes the solubility of passenger proteins, potentially leading to enhancement of proper protein folding. Studies on in vitro refolding in the presence of RNA, coexpression of RNA molecules in vivo and the mutants with impaired RNA binding ability suggests that RNA can exert chaperoning effect on their bound proteins. The results suggest that RNA binding could affect the overall kinetic network of protein folding pathway in favor of productive folding over off-pathway aggregation. In addition, the RNA binding-mediated solubility enhancement is extremely robust for increasing soluble yield of passenger proteins and could be usefully implemented for high-throughput protein expression for functional and structural genomic research initiatives. The RNA-mediated chaperone type presented here would give new insights Into de novo folding in vivo.

Original languageEnglish
Article numbere2677
JournalPloS one
Volume3
Issue number7
DOIs
Publication statusPublished - 2008 Jul 16

Fingerprint

protein folding
protein solubility
Protein Folding
Solubility
RNA
Proteins
Protein folding
proteins
solubility
Agglomeration
molecular chaperones
Molecular Chaperones
binding capacity
Machinery
protein synthesis
genomics
Throughput
kinetics
mutants
Molecules

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Choi, S. I., Han, K. S., Kim, C. W., Ryu, K. S., Kim, B. H., Kim, K. H., ... Seong, B. L. (2008). Protein solubility and folding enhancement by interaction with RNA. PloS one, 3(7), [e2677]. https://doi.org/10.1371/journal.pone.0002677
Choi, Seong Il ; Han, Kyoung Sim ; Kim, Chul Woo ; Ryu, Ki Sun ; Kim, Byung Hee ; Kim, Kyun Hwan ; Kim, Seo Il ; Kang, Tae Hyun ; Shin, Hang Cheol ; Lim, Keo Heun ; Kim, Hyo Kyung ; Hyun, Jeong Min ; Seong, Baik Lin. / Protein solubility and folding enhancement by interaction with RNA. In: PloS one. 2008 ; Vol. 3, No. 7.
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Choi, SI, Han, KS, Kim, CW, Ryu, KS, Kim, BH, Kim, KH, Kim, SI, Kang, TH, Shin, HC, Lim, KH, Kim, HK, Hyun, JM & Seong, BL 2008, 'Protein solubility and folding enhancement by interaction with RNA', PloS one, vol. 3, no. 7, e2677. https://doi.org/10.1371/journal.pone.0002677

Protein solubility and folding enhancement by interaction with RNA. / Choi, Seong Il; Han, Kyoung Sim; Kim, Chul Woo; Ryu, Ki Sun; Kim, Byung Hee; Kim, Kyun Hwan; Kim, Seo Il; Kang, Tae Hyun; Shin, Hang Cheol; Lim, Keo Heun; Kim, Hyo Kyung; Hyun, Jeong Min; Seong, Baik Lin.

In: PloS one, Vol. 3, No. 7, e2677, 16.07.2008.

Research output: Contribution to journalArticle

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AU - Kim, Seo Il

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Choi SI, Han KS, Kim CW, Ryu KS, Kim BH, Kim KH et al. Protein solubility and folding enhancement by interaction with RNA. PloS one. 2008 Jul 16;3(7). e2677. https://doi.org/10.1371/journal.pone.0002677