Protein tyrosine kinase 7 has a conserved role in Wnt/β-catenin canonical signalling

Francesca Puppo, Virginie Thomé, Anne Catherine Lhoumeau, Marie Cibois, Akanksha Gangar, Frédérique Lembo, Edwige Belotti, Sylvie Marchetto, Patrick Lécine, Thomas Prébet, Michael Sebbagh, Won Sik Shin, Seung Taek Lee, Laurent Kodjabachian, Jean Paul Borg

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62 Citations (Scopus)

Abstract

The receptor protein tyrosine kinase 7 (PTK7) was recently shown to participate in noncanonical Wnt/planar cell polarity signalling during mouse and frog embryonic development. In this study, we report that PTK7 interacts with β-catenin in a yeast two-hybrid assay and mammalian cells. PTK7-deficient cells exhibit weakenedβ-catenin/T-cell factor transcriptional activity on Wnt3a stimulation. Furthermore, Xenopus PTK7 is required for the formation of Spemann's organizer and for Siamois promoter activation, events that require Î 2-catenin transcriptional activity. Using epistatic assays, we demonstrate that PTK7 functions upstream from glycogen synthase kinase 3. Taken together, our data reveal a new and conserved role for PTK7 in the Wnt canonical signalling pathway.

Original languageEnglish
Pages (from-to)43-49
Number of pages7
JournalEMBO Reports
Volume12
Issue number1
DOIs
Publication statusPublished - 2011 Jan 1

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Puppo, F., Thomé, V., Lhoumeau, A. C., Cibois, M., Gangar, A., Lembo, F., Belotti, E., Marchetto, S., Lécine, P., Prébet, T., Sebbagh, M., Shin, W. S., Lee, S. T., Kodjabachian, L., & Borg, J. P. (2011). Protein tyrosine kinase 7 has a conserved role in Wnt/β-catenin canonical signalling. EMBO Reports, 12(1), 43-49. https://doi.org/10.1038/embor.2010.185