Proteomic analyses reveal that ginsenoside Rg3(S) partially reverses cellular senescence in human dermal fibroblasts by inducing peroxiredoxin

Ik Soon Jang; Eunbi Jo; Soo Jung Park; Su Jeong Baek; In Hu Hwang; Hyun Mi Kang; Je Ho Lee; Joseph Kwon; Junik Son; Ho Jeong Kwon; Jong Soon Choi

doi:10.1016/j.jgr.2018.07.008

Proteomic analyses reveal that ginsenoside Rg3(S) partially reverses cellular senescence in human dermal fibroblasts by inducing peroxiredoxin

Ik Soon Jang, Eunbi Jo, Soo Jung Park, Su Jeong Baek, In Hu Hwang, Hyun Mi Kang, Je Ho Lee, Joseph Kwon, Junik Son, Ho Jeong Kwon, Jong Soon Choi

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Background: The cellular senescence of primary cultured cells is an irreversible process characterized by growth arrest. Restoration of senescence by ginsenosides has not been explored so far. Rg3(S) treatment markedly decreased senescence-associated β-galactosidase activity and intracellular reactive oxygen species levels in senescent human dermal fibroblasts (HDFs). However, the underlying mechanism of this effect of Rg3(S) on the senescent HDFs remains unknown. Methods: We performed a label-free quantitative proteomics to identify the altered proteins in Rg3(S)-treated senescent HDFs. Upregulated proteins induced by Rg3(S) were validated by real-time polymerase chain reaction and immunoblot analyses. Results: Finally, 157 human proteins were identified, and variable peroxiredoxin (PRDX) isotypes were highly implicated by network analyses. Among them, the mitochondrial PRDX3 was transcriptionally and translationally increased in response to Rg3(S) treatment in senescent HDFs in a time-dependent manner. Conclusion: Our proteomic approach provides insights into the partial reversing effect of Rg3 on senescent HDFs through induction of antioxidant enzymes, particularly PRDX3.

Original language	English
Pages (from-to)	50-57
Number of pages	8
Journal	Journal of Ginseng Research
Volume	44
Issue number	1
DOIs	https://doi.org/10.1016/j.jgr.2018.07.008
Publication status	Published - 2020 Jan

Bibliographical note

Funding Information:
The present study was supported by the Korea Basic Science Institute research grant ( C37975 ) and ( D36403 ) awarded to J.S. Choi. This work was partly supported by the Korean Government ( MEST ; 2015K1A1A2028365 , 2015M3A9C4076321 ) through the Brain Korea 21 Plus Project, Republic of Korea, which was awarded to H.J. Kwon.

Funding Information:
The present study was supported by the Korea Basic Science Institute research grant (C39712) awarded to J.S. Choi and 2017R1D1A1B03034936. Also, this work was supported by Innopolis Foundation through Technology Commercialization services, funded by Ministry of Science and ICT (2019JBRD0004) to I.S. Jang. This work was partly supported by the Korean Government (MEST; 2015K1A1A2028365, 2015M3A9C 4076321) through the Brain Korea 21 Plus Project, Republic of Korea, which was awarded to H.J. Kwon.

Publisher Copyright:
© 2018

All Science Journal Classification (ASJC) codes

Biotechnology
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Complementary and alternative medicine

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10.1016/j.jgr.2018.07.008

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@article{98d2a8b14ad941d9a6c41e1f30ceee77,

title = "Proteomic analyses reveal that ginsenoside Rg3(S) partially reverses cellular senescence in human dermal fibroblasts by inducing peroxiredoxin",

abstract = "Background: The cellular senescence of primary cultured cells is an irreversible process characterized by growth arrest. Restoration of senescence by ginsenosides has not been explored so far. Rg3(S) treatment markedly decreased senescence-associated β-galactosidase activity and intracellular reactive oxygen species levels in senescent human dermal fibroblasts (HDFs). However, the underlying mechanism of this effect of Rg3(S) on the senescent HDFs remains unknown. Methods: We performed a label-free quantitative proteomics to identify the altered proteins in Rg3(S)-treated senescent HDFs. Upregulated proteins induced by Rg3(S) were validated by real-time polymerase chain reaction and immunoblot analyses. Results: Finally, 157 human proteins were identified, and variable peroxiredoxin (PRDX) isotypes were highly implicated by network analyses. Among them, the mitochondrial PRDX3 was transcriptionally and translationally increased in response to Rg3(S) treatment in senescent HDFs in a time-dependent manner. Conclusion: Our proteomic approach provides insights into the partial reversing effect of Rg3 on senescent HDFs through induction of antioxidant enzymes, particularly PRDX3.",

author = "Jang, {Ik Soon} and Eunbi Jo and Park, {Soo Jung} and Baek, {Su Jeong} and Hwang, {In Hu} and Kang, {Hyun Mi} and Lee, {Je Ho} and Joseph Kwon and Junik Son and Kwon, {Ho Jeong} and Choi, {Jong Soon}",

note = "Funding Information: The present study was supported by the Korea Basic Science Institute research grant ( C37975 ) and ( D36403 ) awarded to J.S. Choi. This work was partly supported by the Korean Government ( MEST ; 2015K1A1A2028365 , 2015M3A9C4076321 ) through the Brain Korea 21 Plus Project, Republic of Korea, which was awarded to H.J. Kwon. Funding Information: The present study was supported by the Korea Basic Science Institute research grant (C39712) awarded to J.S. Choi and 2017R1D1A1B03034936. Also, this work was supported by Innopolis Foundation through Technology Commercialization services, funded by Ministry of Science and ICT (2019JBRD0004) to I.S. Jang. This work was partly supported by the Korean Government (MEST; 2015K1A1A2028365, 2015M3A9C 4076321) through the Brain Korea 21 Plus Project, Republic of Korea, which was awarded to H.J. Kwon. Publisher Copyright: {\textcopyright} 2018",

year = "2020",

month = jan,

doi = "10.1016/j.jgr.2018.07.008",

language = "English",

volume = "44",

pages = "50--57",

journal = "Journal of Ginseng Research",

issn = "1226-8453",

publisher = "The Korean Society of Ginseng",

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}

TY - JOUR

T1 - Proteomic analyses reveal that ginsenoside Rg3(S) partially reverses cellular senescence in human dermal fibroblasts by inducing peroxiredoxin

AU - Jang, Ik Soon

AU - Jo, Eunbi

AU - Park, Soo Jung

AU - Baek, Su Jeong

AU - Hwang, In Hu

AU - Kang, Hyun Mi

AU - Lee, Je Ho

AU - Kwon, Joseph

AU - Son, Junik

AU - Kwon, Ho Jeong

AU - Choi, Jong Soon

N1 - Funding Information: The present study was supported by the Korea Basic Science Institute research grant ( C37975 ) and ( D36403 ) awarded to J.S. Choi. This work was partly supported by the Korean Government ( MEST ; 2015K1A1A2028365 , 2015M3A9C4076321 ) through the Brain Korea 21 Plus Project, Republic of Korea, which was awarded to H.J. Kwon. Funding Information: The present study was supported by the Korea Basic Science Institute research grant (C39712) awarded to J.S. Choi and 2017R1D1A1B03034936. Also, this work was supported by Innopolis Foundation through Technology Commercialization services, funded by Ministry of Science and ICT (2019JBRD0004) to I.S. Jang. This work was partly supported by the Korean Government (MEST; 2015K1A1A2028365, 2015M3A9C 4076321) through the Brain Korea 21 Plus Project, Republic of Korea, which was awarded to H.J. Kwon. Publisher Copyright: © 2018

PY - 2020/1

Y1 - 2020/1

N2 - Background: The cellular senescence of primary cultured cells is an irreversible process characterized by growth arrest. Restoration of senescence by ginsenosides has not been explored so far. Rg3(S) treatment markedly decreased senescence-associated β-galactosidase activity and intracellular reactive oxygen species levels in senescent human dermal fibroblasts (HDFs). However, the underlying mechanism of this effect of Rg3(S) on the senescent HDFs remains unknown. Methods: We performed a label-free quantitative proteomics to identify the altered proteins in Rg3(S)-treated senescent HDFs. Upregulated proteins induced by Rg3(S) were validated by real-time polymerase chain reaction and immunoblot analyses. Results: Finally, 157 human proteins were identified, and variable peroxiredoxin (PRDX) isotypes were highly implicated by network analyses. Among them, the mitochondrial PRDX3 was transcriptionally and translationally increased in response to Rg3(S) treatment in senescent HDFs in a time-dependent manner. Conclusion: Our proteomic approach provides insights into the partial reversing effect of Rg3 on senescent HDFs through induction of antioxidant enzymes, particularly PRDX3.

AB - Background: The cellular senescence of primary cultured cells is an irreversible process characterized by growth arrest. Restoration of senescence by ginsenosides has not been explored so far. Rg3(S) treatment markedly decreased senescence-associated β-galactosidase activity and intracellular reactive oxygen species levels in senescent human dermal fibroblasts (HDFs). However, the underlying mechanism of this effect of Rg3(S) on the senescent HDFs remains unknown. Methods: We performed a label-free quantitative proteomics to identify the altered proteins in Rg3(S)-treated senescent HDFs. Upregulated proteins induced by Rg3(S) were validated by real-time polymerase chain reaction and immunoblot analyses. Results: Finally, 157 human proteins were identified, and variable peroxiredoxin (PRDX) isotypes were highly implicated by network analyses. Among them, the mitochondrial PRDX3 was transcriptionally and translationally increased in response to Rg3(S) treatment in senescent HDFs in a time-dependent manner. Conclusion: Our proteomic approach provides insights into the partial reversing effect of Rg3 on senescent HDFs through induction of antioxidant enzymes, particularly PRDX3.

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U2 - 10.1016/j.jgr.2018.07.008

DO - 10.1016/j.jgr.2018.07.008

M3 - Article

AN - SCOPUS:85053134607

VL - 44

SP - 50

EP - 57

JO - Journal of Ginseng Research

JF - Journal of Ginseng Research

SN - 1226-8453

IS - 1

ER -

Proteomic analyses reveal that ginsenoside Rg3(S) partially reverses cellular senescence in human dermal fibroblasts by inducing peroxiredoxin

Abstract

Bibliographical note

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