Proteomic analysis and molecular characterization of tissue ferritin light chain in hepatocellular carcinoma

Kang Sik Park, Hoguen Kim, Nam Gyun Kim, Sang Yun Cho, Kun Ho Choi, Je Kyung Seong, Young-Ki Paik

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

To investigate a molecular basis for iron depletion in human hepatocellular carcinoma (HCC), 19 cases of HCC were analyzed by two-dimensional electrophoresis (2DE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). Results were compared with those of paired adjacent nontumorous liver tissues. Comparative analysis of the respective spot patterns in 2DE showed that tissue ferritin light chain (T-FLC), an iron-storage protein, was either severely suppressed or reduced to undetectable levels in HCC, which was further supported by Western blot and immunohistochemical analysis. In contrast, transferrin receptor (TfR) was shown to be overexpressed in the same HCC samples. Interestingly, the relative levels of messenger RNA (mRNA) expression of T-FLC in HCC, which were measured by a real-time quantitative reverse-transcription polymerase chain reaction (PCR), exhibited almost the same levels as those in normal tissues, suggesting that the translational or posttranslational modification of T-FLC may be the cause of T-FLC suppression in HCC. Furthermore, with PCR-based loss of heterozygosity analysis, only 1 of 19 HCCs showed chromosomal deletions at 19q13.3-q13.4 where T-FLC is located, indicating that the suppression of T-FLC is unlikely due to structural genomic changes with HCC. In conclusion, both proteomic and genomic evidence support not only a basis for the suppression of T-FLC in HCC but also provide a new clue to the unresolved question of iron depletion during hepatocarcinogenesis.

Original languageEnglish
Pages (from-to)1459-1466
Number of pages8
JournalHepatology
Volume35
Issue number6
DOIs
Publication statusPublished - 2002 Jan 1

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Apoferritins
Proteomics
Hepatocellular Carcinoma
Iron
Far-Western Blotting
Polymerase Chain Reaction
Transferrin Receptors
Loss of Heterozygosity
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Post Translational Protein Processing
Reverse Transcription
Electrophoresis

All Science Journal Classification (ASJC) codes

  • Hepatology

Cite this

Park, Kang Sik ; Kim, Hoguen ; Kim, Nam Gyun ; Cho, Sang Yun ; Choi, Kun Ho ; Seong, Je Kyung ; Paik, Young-Ki. / Proteomic analysis and molecular characterization of tissue ferritin light chain in hepatocellular carcinoma. In: Hepatology. 2002 ; Vol. 35, No. 6. pp. 1459-1466.
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abstract = "To investigate a molecular basis for iron depletion in human hepatocellular carcinoma (HCC), 19 cases of HCC were analyzed by two-dimensional electrophoresis (2DE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). Results were compared with those of paired adjacent nontumorous liver tissues. Comparative analysis of the respective spot patterns in 2DE showed that tissue ferritin light chain (T-FLC), an iron-storage protein, was either severely suppressed or reduced to undetectable levels in HCC, which was further supported by Western blot and immunohistochemical analysis. In contrast, transferrin receptor (TfR) was shown to be overexpressed in the same HCC samples. Interestingly, the relative levels of messenger RNA (mRNA) expression of T-FLC in HCC, which were measured by a real-time quantitative reverse-transcription polymerase chain reaction (PCR), exhibited almost the same levels as those in normal tissues, suggesting that the translational or posttranslational modification of T-FLC may be the cause of T-FLC suppression in HCC. Furthermore, with PCR-based loss of heterozygosity analysis, only 1 of 19 HCCs showed chromosomal deletions at 19q13.3-q13.4 where T-FLC is located, indicating that the suppression of T-FLC is unlikely due to structural genomic changes with HCC. In conclusion, both proteomic and genomic evidence support not only a basis for the suppression of T-FLC in HCC but also provide a new clue to the unresolved question of iron depletion during hepatocarcinogenesis.",
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Proteomic analysis and molecular characterization of tissue ferritin light chain in hepatocellular carcinoma. / Park, Kang Sik; Kim, Hoguen; Kim, Nam Gyun; Cho, Sang Yun; Choi, Kun Ho; Seong, Je Kyung; Paik, Young-Ki.

In: Hepatology, Vol. 35, No. 6, 01.01.2002, p. 1459-1466.

Research output: Contribution to journalArticle

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AU - Park, Kang Sik

AU - Kim, Hoguen

AU - Kim, Nam Gyun

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