Proteomic analysis distinguishes basaloid carcinoma as a distinct subtype of nonsmall cell lung carcinoma

Shan Li Long, Hyunki Kim, Hwanseok Rhee, Hoon Kim Se, Hwan Shin Dong, Young Chung Kyung, Kang Sik Park, Young Ki Paik, Joon Chang, Hoguen Kim

Research output: Contribution to journalArticle

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Abstract

The histopathologic type of lung cancer is known to be correlated with tumor behavior and prognosis. However, this classification is subjective and no specific molecular markers have been identified. The aim of this study was to identify protein markers in different types of nonsmall cell lung cancers. Two-dimensional polyacrylamide gel electrophoresis analysis was performed with paired samples of three squamous cell carcinomas, three adenocarcinomas, four large cell carcinomas, and four basaloid carcinomas. We found that 25 proteins in 14 cases of lung cancer were differentially expressed compared to matched nontumorous lung tissues. Among these 25 proteins, 11 proteins were down-regulated and 14 were up-regulated in these four types of lung cancer. Alloalbumin venezia, selenium-binding protein 1, carbonic dehydratase, heat shock 20KD-like protein, and SM22 α protein were down-regulated in all 14 cases of lung cancer examined, whereas alpha enolase was consistently up-regulated. Supervised hierarchical cluster analysis based on the 25 differentially expressed proteins showed that basaloid carcinoma formed one independent group, whereas the other three cancer types were not uniquely classifiable. Our findings suggest that basaloid carcinoma is a unique subtype of nonsmall cell lung carcinoma.

Original languageEnglish
Pages (from-to)3394-3400
Number of pages7
JournalProteomics
Volume4
Issue number11
DOIs
Publication statusPublished - 2004 Nov 1

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Proteomics
Cells
Carcinoma
Lung
Lung Neoplasms
Proteins
Selenium-Binding Proteins
Hydro-Lyases
Large Cell Carcinoma
Phosphopyruvate Hydratase
Electrophoresis, Gel, Two-Dimensional
Cluster analysis
Electrophoresis
Non-Small Cell Lung Carcinoma
Cluster Analysis
Tumors
Squamous Cell Carcinoma
Shock
Neoplasms
Adenocarcinoma

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Long, Shan Li ; Kim, Hyunki ; Rhee, Hwanseok ; Se, Hoon Kim ; Dong, Hwan Shin ; Kyung, Young Chung ; Park, Kang Sik ; Paik, Young Ki ; Chang, Joon ; Kim, Hoguen. / Proteomic analysis distinguishes basaloid carcinoma as a distinct subtype of nonsmall cell lung carcinoma. In: Proteomics. 2004 ; Vol. 4, No. 11. pp. 3394-3400.
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abstract = "The histopathologic type of lung cancer is known to be correlated with tumor behavior and prognosis. However, this classification is subjective and no specific molecular markers have been identified. The aim of this study was to identify protein markers in different types of nonsmall cell lung cancers. Two-dimensional polyacrylamide gel electrophoresis analysis was performed with paired samples of three squamous cell carcinomas, three adenocarcinomas, four large cell carcinomas, and four basaloid carcinomas. We found that 25 proteins in 14 cases of lung cancer were differentially expressed compared to matched nontumorous lung tissues. Among these 25 proteins, 11 proteins were down-regulated and 14 were up-regulated in these four types of lung cancer. Alloalbumin venezia, selenium-binding protein 1, carbonic dehydratase, heat shock 20KD-like protein, and SM22 α protein were down-regulated in all 14 cases of lung cancer examined, whereas alpha enolase was consistently up-regulated. Supervised hierarchical cluster analysis based on the 25 differentially expressed proteins showed that basaloid carcinoma formed one independent group, whereas the other three cancer types were not uniquely classifiable. Our findings suggest that basaloid carcinoma is a unique subtype of nonsmall cell lung carcinoma.",
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Long, SL, Kim, H, Rhee, H, Se, HK, Dong, HS, Kyung, YC, Park, KS, Paik, YK, Chang, J & Kim, H 2004, 'Proteomic analysis distinguishes basaloid carcinoma as a distinct subtype of nonsmall cell lung carcinoma', Proteomics, vol. 4, no. 11, pp. 3394-3400. https://doi.org/10.1002/pmic.200400901

Proteomic analysis distinguishes basaloid carcinoma as a distinct subtype of nonsmall cell lung carcinoma. / Long, Shan Li; Kim, Hyunki; Rhee, Hwanseok; Se, Hoon Kim; Dong, Hwan Shin; Kyung, Young Chung; Park, Kang Sik; Paik, Young Ki; Chang, Joon; Kim, Hoguen.

In: Proteomics, Vol. 4, No. 11, 01.11.2004, p. 3394-3400.

Research output: Contribution to journalArticle

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AU - Dong, Hwan Shin

AU - Kyung, Young Chung

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AU - Paik, Young Ki

AU - Chang, Joon

AU - Kim, Hoguen

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