Proteomic analysis of acetylation in thermophilic Geobacillus kaustophilus

Dong Woo Lee, Dooil Kim, Yong Jik Lee, Jung Ae Kim, Ji Young Choi, Sunghyun Kang, Jae Gu Pan

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Recent analysis of prokaryotic Nε-lysine-acetylated proteins highlights the posttranslational regulation of a broad spectrum of cellular proteins. However, the exact role of acetylation remains unclear due to a lack of acetylated proteome data in prokaryotes. Here, we present the Nε-lysine-acetylated proteome of gram-positive thermophilic Geobacillus kaustophilus. Affinity enrichment using acetyl-lysine-specific antibodies followed by LC-MS/MS analysis revealed 253 acetylated peptides representing 114 proteins. These acetylated proteins include not only common orthologs from mesophilic Bacillus counterparts, but also unique G. kaustophilus proteins, indicating that lysine acetylation is pronounced in thermophilic bacteria. These data complement current knowledge of the bacterial acetylproteome and provide an expanded platform for better understanding of the function of acetylation in cellular metabolism.

Original languageEnglish
Pages (from-to)2278-2282
Number of pages5
JournalProteomics
Volume13
Issue number15
DOIs
Publication statusPublished - 2013 Aug 1

Fingerprint

Geobacillus
Acetylation
Proteomics
Lysine
Proteome
Proteins
Bacilli
Metabolism
Bacillus
Bacteria
Peptides
Antibodies

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Lee, D. W., Kim, D., Lee, Y. J., Kim, J. A., Choi, J. Y., Kang, S., & Pan, J. G. (2013). Proteomic analysis of acetylation in thermophilic Geobacillus kaustophilus. Proteomics, 13(15), 2278-2282. https://doi.org/10.1002/pmic.201200072
Lee, Dong Woo ; Kim, Dooil ; Lee, Yong Jik ; Kim, Jung Ae ; Choi, Ji Young ; Kang, Sunghyun ; Pan, Jae Gu. / Proteomic analysis of acetylation in thermophilic Geobacillus kaustophilus. In: Proteomics. 2013 ; Vol. 13, No. 15. pp. 2278-2282.
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Lee, DW, Kim, D, Lee, YJ, Kim, JA, Choi, JY, Kang, S & Pan, JG 2013, 'Proteomic analysis of acetylation in thermophilic Geobacillus kaustophilus', Proteomics, vol. 13, no. 15, pp. 2278-2282. https://doi.org/10.1002/pmic.201200072

Proteomic analysis of acetylation in thermophilic Geobacillus kaustophilus. / Lee, Dong Woo; Kim, Dooil; Lee, Yong Jik; Kim, Jung Ae; Choi, Ji Young; Kang, Sunghyun; Pan, Jae Gu.

In: Proteomics, Vol. 13, No. 15, 01.08.2013, p. 2278-2282.

Research output: Contribution to journalArticle

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