Proteomic analysis of Caenorhabditis elegans.

Pan Young Jeong, Keun Na, Mi Jeong Jeong, David Chitwood, Yhong Hee Shim, Young Ki Paik

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Proteomic studies of the free-living nematode Caenorhabditis elegans have recently received great attention because this animal model is a useful platform for the in vivo study of various biological problems relevant to human disease. In general, proteomic analysis is carried out in order to address a specific question with respect to differential changes in proteome expression under certain perturbed conditions. In this chapter, we focus on gel-based proteomic analysis of C. elegans subjected to two specific stress conditions during development: induction of the dauer state for whole body protein expression and a temperature shift for egg protein expression. Utilizing these differently perturbed C. elegans protein samples, two-dimensional electrophoresis and differential in-gel electrophoresis methods have led to the discovery of remarkable aspects of the worm's biology. We also provide numerous details about the technical points and protocols necessary for successful experimentation.

Original languageEnglish
Pages (from-to)145-169
Number of pages25
JournalMethods in molecular biology (Clifton, N.J.)
Volume519
DOIs
Publication statusPublished - 2009

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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