Proteomic analysis of resting and activated human CD8+ T cells

Jung Hui Koo, Wook Jin Chae, Je Min Choi, Hyung Wook Nam, Tomohiro Morio, Yu Sam Kim, Yang Soo Jang, Kwan Yong Choi, Jung Jin Yang, Sang Kyou Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

CD8+ T lymphocytes with the cytotoxic activity and capability to release various cytokines are the major players in immune responses against viral infection and cancer. To identify the proteins specific to resting or activated human CD8+ T cells, human CD8+ T cells were activated with anti-CD3+anti-CD28 mAb in the presence of IL-2. The solubilized proteins from resting and activated human CD8+ T cells were separated by high-resolution two-dimensional polyacrylamide gel electrophoresis, and their proteomes were analyzed. Proteomic analysis of resting and activated T cells resulted in identification of 35 proteins with the altered expression. Mass spectrometry coupled with Profound and SWISS-PROT database analysis revealed that these identified proteins are to be functionally associated with cell proliferation, metabolic pathways, antigen presentation, and intracellular signal transduction pathways. We also identified six unknown proteins predicted from genomic DNA sequences specific to resting or activated CD8+ T cells. Protein network studies and functional characterization of these novel proteins may provide new insight into the signaling transduction pathway of CD8+ T cell activation.

Original languageEnglish
Pages (from-to)911-920
Number of pages10
JournalJournal of microbiology and biotechnology
Volume16
Issue number6
Publication statusPublished - 2006 Jun 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

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    Koo, J. H., Chae, W. J., Choi, J. M., Nam, H. W., Morio, T., Kim, Y. S., Jang, Y. S., Choi, K. Y., Yang, J. J., & Lee, S. K. (2006). Proteomic analysis of resting and activated human CD8+ T cells. Journal of microbiology and biotechnology, 16(6), 911-920.