Proteomic analysis of the sterol-mediated signaling pathway in Caenorhabditis elegans.

Byung Kwon Choi, Yun Kyung Shin, Eun Young Lee, Pan Young Jeong, Yhong Hee Shim, David J. Chitwood, Young Ki Paik

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Abstract

Since Caenorhabditis elegans is incapable of de novo cholesterol biosynthesis, it must utilize other nonpermissive sterols that are present in the environment by converting them into cholesterol for cellular function. The inhibition of sterol conversion to cholesterol in C. elegans by various sterol biosynthesis inhibitors (SBIs) is known to cause serious defects in the development of these worms. To determine the biochemical consequences of these physiological abnormalities, one can perform a proteomic analysis of worms of a certain stage that are grown in the presence of SBIs in order for the differential expression of proteins involved in the sterol-mediated signaling pathway to be identified. For example, reductions in the expression of lipoprotein family members, such as vitellogenin-2 and vitellogenin-6, are prominent in azacoprostane-treated worms. This phenomenon is also seen in worms treated with AY-9944, which blocks the conversion of 7-dehydrocholesterol, a major sterol present in C. elegans, to cholesterol.

Original languageEnglish
Pages (from-to)181-195
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Volume462
Publication statusPublished - 2009

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Choi, B. K., Shin, Y. K., Lee, E. Y., Jeong, P. Y., Shim, Y. H., Chitwood, D. J., & Paik, Y. K. (2009). Proteomic analysis of the sterol-mediated signaling pathway in Caenorhabditis elegans. Methods in molecular biology (Clifton, N.J.), 462, 181-195.