Proteomic and transcriptomic analysis of interleukin-1β treated lung carcinoma cell line

Chang Hoon Kim, Do Kyun Kim, Seung Jin Choi, Kun Ho Choi, Kyoung Seob Song, Janghoon Chi, Ja Seok Koo, Seung Yong Hwang, Joo Heon Yoon, Je Kyung Seong

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Mucin hypersecretion is one of the main symptoms of inflammatory disease in the respiratory tract. We previously reported that the pleiotypic pro-inflammatory cytokine, interleukin (IL)-1β, plays a significant role in respiratory tract inflammation by inducing mucins. However, the molecular mechanism for mucin hypersecretion in the respiratory tract remains still unclear. In order to understand the mechanisms of mucin hypersecretion in the airway epithelium, the differentially expressed proteins and genes in the lung mucoepidermoid carcinoma cell line (NCI-H292 cells), which were treated for 6 and 24 hours with IL-1β (10 ng/mL) were identified using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) proteomics and cDNA microarray analysis (8.6K). In the 2-D PAGE, eight differentially expressed proteins and 14 post-translational modification proteins were identified at 6 and 24 hours after the IL-1β-treatment. Four hundred and thirteen genes (6.6%) and 115 genes (2.0%) were differentially expressed, respectively, at 6 and 24 hours after the IL-1β-treatment by microarray analysis. The differentially expressed genes and proteins that were regulated by the IL-1β-treatment were mostly in the metabolic pathway rather than in the regulatory pathway. These results clearly show that the transcript levels have little value in predicting the extent of protein expression.

Original languageEnglish
Pages (from-to)2454-2471
Number of pages18
JournalProteomics
Volume3
Issue number12
DOIs
Publication statusPublished - 2003 Dec 1

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Interleukin-1
Proteomics
Mucins
Cells
Carcinoma
Cell Line
Lung
Microarray Analysis
Microarrays
Proteins
Respiratory System
Genes
Mucoepidermoid Carcinoma
Respiratory Tract Diseases
Electrophoresis, Gel, Two-Dimensional
Post Translational Protein Processing
Metabolic Networks and Pathways
Oligonucleotide Array Sequence Analysis
Electrophoresis
Interleukin-10

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Kim, C. H., Kim, D. K., Choi, S. J., Choi, K. H., Song, K. S., Chi, J., ... Seong, J. K. (2003). Proteomic and transcriptomic analysis of interleukin-1β treated lung carcinoma cell line. Proteomics, 3(12), 2454-2471. https://doi.org/10.1002/pmic.200300643
Kim, Chang Hoon ; Kim, Do Kyun ; Choi, Seung Jin ; Choi, Kun Ho ; Song, Kyoung Seob ; Chi, Janghoon ; Koo, Ja Seok ; Hwang, Seung Yong ; Yoon, Joo Heon ; Seong, Je Kyung. / Proteomic and transcriptomic analysis of interleukin-1β treated lung carcinoma cell line. In: Proteomics. 2003 ; Vol. 3, No. 12. pp. 2454-2471.
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abstract = "Mucin hypersecretion is one of the main symptoms of inflammatory disease in the respiratory tract. We previously reported that the pleiotypic pro-inflammatory cytokine, interleukin (IL)-1β, plays a significant role in respiratory tract inflammation by inducing mucins. However, the molecular mechanism for mucin hypersecretion in the respiratory tract remains still unclear. In order to understand the mechanisms of mucin hypersecretion in the airway epithelium, the differentially expressed proteins and genes in the lung mucoepidermoid carcinoma cell line (NCI-H292 cells), which were treated for 6 and 24 hours with IL-1β (10 ng/mL) were identified using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) proteomics and cDNA microarray analysis (8.6K). In the 2-D PAGE, eight differentially expressed proteins and 14 post-translational modification proteins were identified at 6 and 24 hours after the IL-1β-treatment. Four hundred and thirteen genes (6.6{\%}) and 115 genes (2.0{\%}) were differentially expressed, respectively, at 6 and 24 hours after the IL-1β-treatment by microarray analysis. The differentially expressed genes and proteins that were regulated by the IL-1β-treatment were mostly in the metabolic pathway rather than in the regulatory pathway. These results clearly show that the transcript levels have little value in predicting the extent of protein expression.",
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Kim, CH, Kim, DK, Choi, SJ, Choi, KH, Song, KS, Chi, J, Koo, JS, Hwang, SY, Yoon, JH & Seong, JK 2003, 'Proteomic and transcriptomic analysis of interleukin-1β treated lung carcinoma cell line', Proteomics, vol. 3, no. 12, pp. 2454-2471. https://doi.org/10.1002/pmic.200300643

Proteomic and transcriptomic analysis of interleukin-1β treated lung carcinoma cell line. / Kim, Chang Hoon; Kim, Do Kyun; Choi, Seung Jin; Choi, Kun Ho; Song, Kyoung Seob; Chi, Janghoon; Koo, Ja Seok; Hwang, Seung Yong; Yoon, Joo Heon; Seong, Je Kyung.

In: Proteomics, Vol. 3, No. 12, 01.12.2003, p. 2454-2471.

Research output: Contribution to journalArticle

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AU - Kim, Chang Hoon

AU - Kim, Do Kyun

AU - Choi, Seung Jin

AU - Choi, Kun Ho

AU - Song, Kyoung Seob

AU - Chi, Janghoon

AU - Koo, Ja Seok

AU - Hwang, Seung Yong

AU - Yoon, Joo Heon

AU - Seong, Je Kyung

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AB - Mucin hypersecretion is one of the main symptoms of inflammatory disease in the respiratory tract. We previously reported that the pleiotypic pro-inflammatory cytokine, interleukin (IL)-1β, plays a significant role in respiratory tract inflammation by inducing mucins. However, the molecular mechanism for mucin hypersecretion in the respiratory tract remains still unclear. In order to understand the mechanisms of mucin hypersecretion in the airway epithelium, the differentially expressed proteins and genes in the lung mucoepidermoid carcinoma cell line (NCI-H292 cells), which were treated for 6 and 24 hours with IL-1β (10 ng/mL) were identified using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) proteomics and cDNA microarray analysis (8.6K). In the 2-D PAGE, eight differentially expressed proteins and 14 post-translational modification proteins were identified at 6 and 24 hours after the IL-1β-treatment. Four hundred and thirteen genes (6.6%) and 115 genes (2.0%) were differentially expressed, respectively, at 6 and 24 hours after the IL-1β-treatment by microarray analysis. The differentially expressed genes and proteins that were regulated by the IL-1β-treatment were mostly in the metabolic pathway rather than in the regulatory pathway. These results clearly show that the transcript levels have little value in predicting the extent of protein expression.

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