Proteomic profiling of cellular proteins interacting with the hepatitis C virus core protein

Su Min Kang, Min Jung Shin, Jung Hee Ki, Jong Won Oh

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Hepatitis C virus (HCV) is a causative agent of chronic hepatitis and hepatocellular carcinoma. The core protein of HCV packages the viral RNA genome to form a nucleocapsid. In addition to its function as a structural protein, core protein is involved in regulation of cellular transcription, virus-induced transformation, and pathogenesis. To gain insights into cellular functions of the core protein by identification of cellular proteins interacting with the core protein, we employed a proteomic approach. Hepatocytes soluble cytoplasmic proteins were applied to the core proteins immobilized on Ni-nitrilotriacetic resin and total bound cellular proteins were resolved by 2-DE. Analyses of interacting proteins by matrix-assisted laser desorption/ionization-time of flight mass spectrometry allowed identification of 14 cellular proteins binding to the core protein. These proteins include DEAD-box polypeptide 5, similar in function to a known protein identified previously by yeast two-hybrid screening and 13 newly identified cellular proteins. Interestingly, nine protein spots were identified as intermediate microfilament proteins, including cytokeratins (five spots for cytokeratin 8, two for cytokeratin 19, and one for cytokeratin 18) and vimentin. Cytokeratin 8 and vimentin, which were previously shown to be involved in the infection processes of other viruses, were further analyzed to confirm their in vivo interactions with the core protein by immunoblotting and immunofluorescence microscopy. We discuss the functional implications of the interactions of the core protein with newly identified cellular proteins in HCV infection and pathogenesis.

Original languageEnglish
Pages (from-to)2227-2237
Number of pages11
JournalProteomics
Volume5
Issue number8
DOIs
Publication statusPublished - 2005 May 1

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Proteomics
Proteins
Viruses
Keratin-8
Hepatitis C virus nucleocapsid protein
Vimentin
Hepacivirus
Immobilized Proteins
Keratin-18
Keratin-19
Microfilament Proteins
Nucleocapsid
Viral Genome
Viral RNA
Virus Diseases
Chronic Hepatitis
Transcription
Keratins
Fluorescence Microscopy
Immunoblotting

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Kang, Su Min ; Shin, Min Jung ; Ki, Jung Hee ; Oh, Jong Won. / Proteomic profiling of cellular proteins interacting with the hepatitis C virus core protein. In: Proteomics. 2005 ; Vol. 5, No. 8. pp. 2227-2237.
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Proteomic profiling of cellular proteins interacting with the hepatitis C virus core protein. / Kang, Su Min; Shin, Min Jung; Ki, Jung Hee; Oh, Jong Won.

In: Proteomics, Vol. 5, No. 8, 01.05.2005, p. 2227-2237.

Research output: Contribution to journalArticle

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