Proteomics analysis of apoptosis-regulating proteins in tissues with different radiosensitivity

Jeung Hee An, Jinsil Seong

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The aim of this study was to identify of radiosusceptibility proteins in tissues with different radiosensitivity. C3H/HeJ mice were exposed to 10 Gy. The tissues were processed for proteins extraction and were analyzed by 2-dimensional electrophoresis. The proteins were identified by matrix-assisted laser desorption ionizing time-of-flight mass spectrometry and validated by immunohistochemical staining and Western blotting. The peaks of apoptosis levels were 35.3 ± 1.7% and 0.6 ± 0.2% in the spleen and the liver, respectively, after ionizing radiation. Analysis of liver tissue showed that the expression level of ROS related proteins such as cytochrome c, glutathione S transferase, NADH dehydrogenase and peroxiredoxin VI increased after radiation. The expression level of cytochrome c increased to 3-fold after ionizing radiation in both tissues. However in spleen tissue, the expression level of various kinds of apoptosis regulating proteins increased after radiation. These involved iodothyronine, CD 59A glycoprotein precursor, fas antigen and tumor necrosis factor -inducible protein TSG-6nprecursor after radiation. The difference in the apoptosis index between the liver and spleen tissues is closely associated with the expression of various kinds of apoptosis-related proteins. The result suggests that the expression of apoptosis-related protein and redox proteins play important roles in this radiosusceptibility.

Original languageEnglish
Pages (from-to)147-155
Number of pages9
JournalJournal of Radiation Research
Volume47
Issue number2
DOIs
Publication statusPublished - 2006 Jul 17

Fingerprint

Radiation Tolerance
apoptosis
radiation tolerance
Proteomics
proteomics
Apoptosis
proteins
Proteins
spleen
liver
Spleen
cytochromes
cytochrome c
Radiation
Ionizing Radiation
Cytochromes c
ionizing radiation
Liver
Peroxiredoxin VI
radiation

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Radiation

Cite this

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title = "Proteomics analysis of apoptosis-regulating proteins in tissues with different radiosensitivity",
abstract = "The aim of this study was to identify of radiosusceptibility proteins in tissues with different radiosensitivity. C3H/HeJ mice were exposed to 10 Gy. The tissues were processed for proteins extraction and were analyzed by 2-dimensional electrophoresis. The proteins were identified by matrix-assisted laser desorption ionizing time-of-flight mass spectrometry and validated by immunohistochemical staining and Western blotting. The peaks of apoptosis levels were 35.3 ± 1.7{\%} and 0.6 ± 0.2{\%} in the spleen and the liver, respectively, after ionizing radiation. Analysis of liver tissue showed that the expression level of ROS related proteins such as cytochrome c, glutathione S transferase, NADH dehydrogenase and peroxiredoxin VI increased after radiation. The expression level of cytochrome c increased to 3-fold after ionizing radiation in both tissues. However in spleen tissue, the expression level of various kinds of apoptosis regulating proteins increased after radiation. These involved iodothyronine, CD 59A glycoprotein precursor, fas antigen and tumor necrosis factor -inducible protein TSG-6nprecursor after radiation. The difference in the apoptosis index between the liver and spleen tissues is closely associated with the expression of various kinds of apoptosis-related proteins. The result suggests that the expression of apoptosis-related protein and redox proteins play important roles in this radiosusceptibility.",
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Proteomics analysis of apoptosis-regulating proteins in tissues with different radiosensitivity. / An, Jeung Hee; Seong, Jinsil.

In: Journal of Radiation Research, Vol. 47, No. 2, 17.07.2006, p. 147-155.

Research output: Contribution to journalArticle

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