PTK1, a novel protein kinase required for proliferation of human melanocytes

Kazuhiko Ezoe, Seung Taek Lee, Kathleen M. Strunk, Richard A. Spritz

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

We have identified and characterized PTK1, a novel human non-receptor protein kinase. Partial PTK1 cDNA was initially isolated by RT-PCR from mRNA of normal human melanocytes. Northern blot hybridization detected a 3.8-kb PTK1 mRNA in all tissues and cell types studied. Nucleotide sequence analysis of a full-length PTK1 cDNA showed it to encode an 847-amino acid polypeptide with an amino-terminal SH3 domain, a kinase catalytic domain, a leucine zipper-like domain, and a carboxyl proline-rich domain. The PTK1 kinase domain contains motifs generally considered diagnostic of serine/threonine kinases but also contains amino acids highly conserved among the tyrosine kinases. This suggests that PTK1 is a serine/threonine kinase, but one very closely related to the tyrosine kinase superfamily. When normal human melanocytes were exposed to antisense PTK1 oligonucleotide, cell growth was inhibited, whereas a corresponding sense oligonucleotide had no inhibitory effect. These data indicate that PTK1 plays an important role in the proliferation of normal human melanocytes.

Original languageEnglish
Pages (from-to)935-938
Number of pages4
JournalOncogene
Volume9
Issue number3
Publication statusPublished - 1994 Mar 1

Fingerprint

Melanocytes
Protein Kinases
Protein-Serine-Threonine Kinases
Protein-Tyrosine Kinases
Phosphotransferases
Complementary DNA
Amino Acids
Leucine Zippers
Messenger RNA
src Homology Domains
Antisense Oligonucleotides
Proline
Oligonucleotides
Northern Blotting
Sequence Analysis
Catalytic Domain
Polymerase Chain Reaction
Peptides
Growth

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Ezoe, K., Lee, S. T., Strunk, K. M., & Spritz, R. A. (1994). PTK1, a novel protein kinase required for proliferation of human melanocytes. Oncogene, 9(3), 935-938.
Ezoe, Kazuhiko ; Lee, Seung Taek ; Strunk, Kathleen M. ; Spritz, Richard A. / PTK1, a novel protein kinase required for proliferation of human melanocytes. In: Oncogene. 1994 ; Vol. 9, No. 3. pp. 935-938.
@article{e2918177792e4d24b21b22ab5eed8bbe,
title = "PTK1, a novel protein kinase required for proliferation of human melanocytes",
abstract = "We have identified and characterized PTK1, a novel human non-receptor protein kinase. Partial PTK1 cDNA was initially isolated by RT-PCR from mRNA of normal human melanocytes. Northern blot hybridization detected a 3.8-kb PTK1 mRNA in all tissues and cell types studied. Nucleotide sequence analysis of a full-length PTK1 cDNA showed it to encode an 847-amino acid polypeptide with an amino-terminal SH3 domain, a kinase catalytic domain, a leucine zipper-like domain, and a carboxyl proline-rich domain. The PTK1 kinase domain contains motifs generally considered diagnostic of serine/threonine kinases but also contains amino acids highly conserved among the tyrosine kinases. This suggests that PTK1 is a serine/threonine kinase, but one very closely related to the tyrosine kinase superfamily. When normal human melanocytes were exposed to antisense PTK1 oligonucleotide, cell growth was inhibited, whereas a corresponding sense oligonucleotide had no inhibitory effect. These data indicate that PTK1 plays an important role in the proliferation of normal human melanocytes.",
author = "Kazuhiko Ezoe and Lee, {Seung Taek} and Strunk, {Kathleen M.} and Spritz, {Richard A.}",
year = "1994",
month = "3",
day = "1",
language = "English",
volume = "9",
pages = "935--938",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "3",

}

Ezoe, K, Lee, ST, Strunk, KM & Spritz, RA 1994, 'PTK1, a novel protein kinase required for proliferation of human melanocytes', Oncogene, vol. 9, no. 3, pp. 935-938.

PTK1, a novel protein kinase required for proliferation of human melanocytes. / Ezoe, Kazuhiko; Lee, Seung Taek; Strunk, Kathleen M.; Spritz, Richard A.

In: Oncogene, Vol. 9, No. 3, 01.03.1994, p. 935-938.

Research output: Contribution to journalArticle

TY - JOUR

T1 - PTK1, a novel protein kinase required for proliferation of human melanocytes

AU - Ezoe, Kazuhiko

AU - Lee, Seung Taek

AU - Strunk, Kathleen M.

AU - Spritz, Richard A.

PY - 1994/3/1

Y1 - 1994/3/1

N2 - We have identified and characterized PTK1, a novel human non-receptor protein kinase. Partial PTK1 cDNA was initially isolated by RT-PCR from mRNA of normal human melanocytes. Northern blot hybridization detected a 3.8-kb PTK1 mRNA in all tissues and cell types studied. Nucleotide sequence analysis of a full-length PTK1 cDNA showed it to encode an 847-amino acid polypeptide with an amino-terminal SH3 domain, a kinase catalytic domain, a leucine zipper-like domain, and a carboxyl proline-rich domain. The PTK1 kinase domain contains motifs generally considered diagnostic of serine/threonine kinases but also contains amino acids highly conserved among the tyrosine kinases. This suggests that PTK1 is a serine/threonine kinase, but one very closely related to the tyrosine kinase superfamily. When normal human melanocytes were exposed to antisense PTK1 oligonucleotide, cell growth was inhibited, whereas a corresponding sense oligonucleotide had no inhibitory effect. These data indicate that PTK1 plays an important role in the proliferation of normal human melanocytes.

AB - We have identified and characterized PTK1, a novel human non-receptor protein kinase. Partial PTK1 cDNA was initially isolated by RT-PCR from mRNA of normal human melanocytes. Northern blot hybridization detected a 3.8-kb PTK1 mRNA in all tissues and cell types studied. Nucleotide sequence analysis of a full-length PTK1 cDNA showed it to encode an 847-amino acid polypeptide with an amino-terminal SH3 domain, a kinase catalytic domain, a leucine zipper-like domain, and a carboxyl proline-rich domain. The PTK1 kinase domain contains motifs generally considered diagnostic of serine/threonine kinases but also contains amino acids highly conserved among the tyrosine kinases. This suggests that PTK1 is a serine/threonine kinase, but one very closely related to the tyrosine kinase superfamily. When normal human melanocytes were exposed to antisense PTK1 oligonucleotide, cell growth was inhibited, whereas a corresponding sense oligonucleotide had no inhibitory effect. These data indicate that PTK1 plays an important role in the proliferation of normal human melanocytes.

UR - http://www.scopus.com/inward/record.url?scp=0028140164&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028140164&partnerID=8YFLogxK

M3 - Article

VL - 9

SP - 935

EP - 938

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 3

ER -