Purification and characterization of a thermostable chitinase from Bacillus licheniformis Mb-2

Aris Toharisman, Maggy Thenawidjaja Suhartono, Margarethe Spindler-Barth, Jae-Kwan Hwang, Yu Ryang Pyun

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

A chitinase produced by Bacillus licheniformis MB-2 isolated from Tompaso geothermal springs, Indonesia, was purified and characterized. The extracellular enzyme was isolated by successive hydrophobic interaction, anion exchange, and gel filtration chromatographies. The purified enzyme was a monomer with an apparent molecular weight of 67 kDa. The optimal temperature and pH of the enzyme were 70°C and 6.0, respectively. It was stable below 60°C for 2 h and over a broad pH range of 4.0-11.0 for 4 h. The enzyme was resistant to denaturation by urea (1 M), Tween-20 (1%) and Triton-X (1%), but unstable toward organic solvents such as dimethyl sulphoxide, DMSO, (5%) and polyethylene glycol, PEG, (5%) for 30 min. The enzyme hydrolysed colloidal chitin, glycol chitin, chitosan, and glycol chitosan. The first 13 N-terminal amino acids of the enzyme were determined as SGKNYKIIGYYPS, which is identical to those in chitinases from B. licheniformis and B. circulans.

Original languageEnglish
Pages (from-to)733-738
Number of pages6
JournalWorld Journal of Microbiology and Biotechnology
Volume21
Issue number5
DOIs
Publication statusPublished - 2005 Jul 1

Fingerprint

Chitinases
Enzymes
Dimethyl Sulfoxide
Hot Springs
Chitin
Indonesia
Polysorbates
Hydrophobic and Hydrophilic Interactions
Gel Chromatography
Anions
Urea
Bacillus licheniformis
Molecular Weight
Amino Acids
Temperature

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

Cite this

Toharisman, Aris ; Suhartono, Maggy Thenawidjaja ; Spindler-Barth, Margarethe ; Hwang, Jae-Kwan ; Pyun, Yu Ryang. / Purification and characterization of a thermostable chitinase from Bacillus licheniformis Mb-2. In: World Journal of Microbiology and Biotechnology. 2005 ; Vol. 21, No. 5. pp. 733-738.
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Purification and characterization of a thermostable chitinase from Bacillus licheniformis Mb-2. / Toharisman, Aris; Suhartono, Maggy Thenawidjaja; Spindler-Barth, Margarethe; Hwang, Jae-Kwan; Pyun, Yu Ryang.

In: World Journal of Microbiology and Biotechnology, Vol. 21, No. 5, 01.07.2005, p. 733-738.

Research output: Contribution to journalArticle

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