Putative chemosensitivity predictive genes in colorectal cancer cell lines for anticancer agents

Jae Joon Jung, Hei Cheul Jeung, Jung Ok Lee, Tae Soo Kim, Hyuncheol Chung, SunYoung Rha

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In order to identify genes which could predict chemosensitivity in colorectal cancer, gene expression and chemosensitivity were examined in colorectal cancer cell lines. Gene expression profiling of 5 colorectal cancer and 3 normal cell lines was performed using a 22K spotted oligonucleotide microarray. The IC50s of 17 anticancer drugs were determined using the MTT assay for chemosensitivity. The SOURCE database, KEGG Pathway database, and Molecular Diagnosis Score (MDS) were used for data analysis. Two representative colorectal cancer cell lines were identified which were resistant or sensitive to drugs commonly used for colon cancer treatment (5-FU, irinotecan and topotecan). Six hundred and eighty-three genes that were up- or down-regulated by >4-fold between the two cell lines were selected. Pathway analysis was performed with 147 of the 683 genes using the KEGG Pathway database. This analysis revealed 27 genes in the apoptosis, MAPK signaling, and focal adhesion pathways, which could explain the mechanism of chemosensitivity in colorectal cancer cell lines. In addition, the chemosensitivity of other colorectal cancer and normal cell lines was predictable with the selected 27 genes. These genes may act as putative predictive markers for chemosensitivity in chemo-naive colorectal cancer patients following functional analysis and clinical validation.

Original languageEnglish
Pages (from-to)593-599
Number of pages7
JournalOncology Reports
Volume18
Issue number3
Publication statusPublished - 2007 Sep 1

Fingerprint

Antineoplastic Agents
Colorectal Neoplasms
Cell Line
Genes
irinotecan
Chemical Databases
Databases
Topotecan
Focal Adhesions
Neoplasm Genes
Gene Expression Profiling
Oligonucleotide Array Sequence Analysis
Fluorouracil
Pharmaceutical Preparations
Colonic Neoplasms
Apoptosis
Gene Expression

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Jung, Jae Joon ; Jeung, Hei Cheul ; Lee, Jung Ok ; Kim, Tae Soo ; Chung, Hyuncheol ; Rha, SunYoung. / Putative chemosensitivity predictive genes in colorectal cancer cell lines for anticancer agents. In: Oncology Reports. 2007 ; Vol. 18, No. 3. pp. 593-599.
@article{4618ad58eb2f4a54a720e9e725ed560d,
title = "Putative chemosensitivity predictive genes in colorectal cancer cell lines for anticancer agents",
abstract = "In order to identify genes which could predict chemosensitivity in colorectal cancer, gene expression and chemosensitivity were examined in colorectal cancer cell lines. Gene expression profiling of 5 colorectal cancer and 3 normal cell lines was performed using a 22K spotted oligonucleotide microarray. The IC50s of 17 anticancer drugs were determined using the MTT assay for chemosensitivity. The SOURCE database, KEGG Pathway database, and Molecular Diagnosis Score (MDS) were used for data analysis. Two representative colorectal cancer cell lines were identified which were resistant or sensitive to drugs commonly used for colon cancer treatment (5-FU, irinotecan and topotecan). Six hundred and eighty-three genes that were up- or down-regulated by >4-fold between the two cell lines were selected. Pathway analysis was performed with 147 of the 683 genes using the KEGG Pathway database. This analysis revealed 27 genes in the apoptosis, MAPK signaling, and focal adhesion pathways, which could explain the mechanism of chemosensitivity in colorectal cancer cell lines. In addition, the chemosensitivity of other colorectal cancer and normal cell lines was predictable with the selected 27 genes. These genes may act as putative predictive markers for chemosensitivity in chemo-naive colorectal cancer patients following functional analysis and clinical validation.",
author = "Jung, {Jae Joon} and Jeung, {Hei Cheul} and Lee, {Jung Ok} and Kim, {Tae Soo} and Hyuncheol Chung and SunYoung Rha",
year = "2007",
month = "9",
day = "1",
language = "English",
volume = "18",
pages = "593--599",
journal = "Oncology Reports",
issn = "1021-335X",
publisher = "Spandidos Publications",
number = "3",

}

Putative chemosensitivity predictive genes in colorectal cancer cell lines for anticancer agents. / Jung, Jae Joon; Jeung, Hei Cheul; Lee, Jung Ok; Kim, Tae Soo; Chung, Hyuncheol; Rha, SunYoung.

In: Oncology Reports, Vol. 18, No. 3, 01.09.2007, p. 593-599.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Putative chemosensitivity predictive genes in colorectal cancer cell lines for anticancer agents

AU - Jung, Jae Joon

AU - Jeung, Hei Cheul

AU - Lee, Jung Ok

AU - Kim, Tae Soo

AU - Chung, Hyuncheol

AU - Rha, SunYoung

PY - 2007/9/1

Y1 - 2007/9/1

N2 - In order to identify genes which could predict chemosensitivity in colorectal cancer, gene expression and chemosensitivity were examined in colorectal cancer cell lines. Gene expression profiling of 5 colorectal cancer and 3 normal cell lines was performed using a 22K spotted oligonucleotide microarray. The IC50s of 17 anticancer drugs were determined using the MTT assay for chemosensitivity. The SOURCE database, KEGG Pathway database, and Molecular Diagnosis Score (MDS) were used for data analysis. Two representative colorectal cancer cell lines were identified which were resistant or sensitive to drugs commonly used for colon cancer treatment (5-FU, irinotecan and topotecan). Six hundred and eighty-three genes that were up- or down-regulated by >4-fold between the two cell lines were selected. Pathway analysis was performed with 147 of the 683 genes using the KEGG Pathway database. This analysis revealed 27 genes in the apoptosis, MAPK signaling, and focal adhesion pathways, which could explain the mechanism of chemosensitivity in colorectal cancer cell lines. In addition, the chemosensitivity of other colorectal cancer and normal cell lines was predictable with the selected 27 genes. These genes may act as putative predictive markers for chemosensitivity in chemo-naive colorectal cancer patients following functional analysis and clinical validation.

AB - In order to identify genes which could predict chemosensitivity in colorectal cancer, gene expression and chemosensitivity were examined in colorectal cancer cell lines. Gene expression profiling of 5 colorectal cancer and 3 normal cell lines was performed using a 22K spotted oligonucleotide microarray. The IC50s of 17 anticancer drugs were determined using the MTT assay for chemosensitivity. The SOURCE database, KEGG Pathway database, and Molecular Diagnosis Score (MDS) were used for data analysis. Two representative colorectal cancer cell lines were identified which were resistant or sensitive to drugs commonly used for colon cancer treatment (5-FU, irinotecan and topotecan). Six hundred and eighty-three genes that were up- or down-regulated by >4-fold between the two cell lines were selected. Pathway analysis was performed with 147 of the 683 genes using the KEGG Pathway database. This analysis revealed 27 genes in the apoptosis, MAPK signaling, and focal adhesion pathways, which could explain the mechanism of chemosensitivity in colorectal cancer cell lines. In addition, the chemosensitivity of other colorectal cancer and normal cell lines was predictable with the selected 27 genes. These genes may act as putative predictive markers for chemosensitivity in chemo-naive colorectal cancer patients following functional analysis and clinical validation.

UR - http://www.scopus.com/inward/record.url?scp=38449119056&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38449119056&partnerID=8YFLogxK

M3 - Article

VL - 18

SP - 593

EP - 599

JO - Oncology Reports

JF - Oncology Reports

SN - 1021-335X

IS - 3

ER -