Pyrithione, a zinc ionophore, inhibits NF-κB activation

Chul Hoon Kim, Joo Hee Kim, Seok Jun Moon, Kwang Chul Chung, Chung Y. Hsu, Jeong Taeg Seo, Young Soo Ahn

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Pyrrolidine dithiocarbamate (PDTC) suppresses NF-κB activity and exhibits cytotoxic effects in bovine cerebral endothelial cells (BCECs), and we have previously reported that these PDTC effects were accompanied by an increase in intracellular zinc levels. To further explore the role of zinc in the modulation of NF-κB activation, we studied the effect of pyrithione, a zinc ionophore, on NF-κB activation in BCECs. Pyrithione inhibited NF-κB activity in a time- and dose-dependent manner. Ca-EDTA, but not Zn-EDTA, prevented pyrithione inhibition of NF-κB activity. Pyrithione increased the intracellular zinc level within 15 min. This effect was also abolished by Ca-EDTA, but not by Zn-EDTA. The potency of pyrithione on NF-κB inhibition and zinc influx was approximately one order of magnitude more potent than PDTC. These findings establish the regulatory role of intracellular zinc levels on NF-κB activity in BCECs.

Original languageEnglish
Pages (from-to)505-509
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume259
Issue number3
DOIs
Publication statusPublished - 1999 Jun 16

Fingerprint

Ionophores
Zinc
Edetic Acid
Chemical activation
Endothelial cells
Endothelial Cells
pyrithione
pyrithione zinc
Modulation
pyrrolidine dithiocarbamic acid

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kim, Chul Hoon ; Kim, Joo Hee ; Moon, Seok Jun ; Chung, Kwang Chul ; Hsu, Chung Y. ; Seo, Jeong Taeg ; Ahn, Young Soo. / Pyrithione, a zinc ionophore, inhibits NF-κB activation. In: Biochemical and Biophysical Research Communications. 1999 ; Vol. 259, No. 3. pp. 505-509.
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Pyrithione, a zinc ionophore, inhibits NF-κB activation. / Kim, Chul Hoon; Kim, Joo Hee; Moon, Seok Jun; Chung, Kwang Chul; Hsu, Chung Y.; Seo, Jeong Taeg; Ahn, Young Soo.

In: Biochemical and Biophysical Research Communications, Vol. 259, No. 3, 16.06.1999, p. 505-509.

Research output: Contribution to journalArticle

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