Pyruvate metabolism: A therapeutic opportunity in radiation-induced skin injury

Hyun Yoo, Jeong Wook Kang, Dong Won Lee, SangHo Oh, Yun Sil Lee, Eun Jung Lee, Jaeho Cho

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Ionizing radiation is used to treat a range of cancers. Despite recent technological progress, radiation therapy can damage the skin at the administration site. The specific molecular mechanisms involved in this effect have not been fully characterized. In this study, the effects of pyruvate, on radiation-induced skin injury were investigated, including the role of the pyruvate dehydrogenase kinase 2 (PDK2) signaling pathway. Next generation sequencing (NGS) identified a wide range of gene expression differences between the control and irradiated mice, including reduced expression of PDK2. This was confirmed using Q-PCR. Cell culture studies demonstrated that PDK2 overexpression and a high cellular pyruvate concentration inhibited radiation-induced cytokine expression. Immunohistochemical studies demonstrated radiation-induced skin thickening and gene expression changes. Oral pyruvate treatment markedly downregulated radiation-induced changes in skin thickness and inflammatory cytokine expression. These findings indicated that regulation of the pyruvate metabolic pathway could provide an effective approach to the control of radiation-induced skin damage.

Original languageEnglish
Pages (from-to)504-510
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume460
Issue number3
DOIs
Publication statusPublished - 2015 May 2

Fingerprint

Pyruvic Acid
Metabolism
Skin
Radiation
Wounds and Injuries
Gene expression
Cytokines
Therapeutics
Gene Expression
Ionizing radiation
Radiotherapy
Metabolic Networks and Pathways
Ionizing Radiation
Cell culture
Down-Regulation
Cell Culture Techniques
Polymerase Chain Reaction
pyruvate dehydrogenase (acetyl-transferring) kinase
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yoo, Hyun ; Kang, Jeong Wook ; Lee, Dong Won ; Oh, SangHo ; Lee, Yun Sil ; Lee, Eun Jung ; Cho, Jaeho. / Pyruvate metabolism : A therapeutic opportunity in radiation-induced skin injury. In: Biochemical and Biophysical Research Communications. 2015 ; Vol. 460, No. 3. pp. 504-510.
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Pyruvate metabolism : A therapeutic opportunity in radiation-induced skin injury. / Yoo, Hyun; Kang, Jeong Wook; Lee, Dong Won; Oh, SangHo; Lee, Yun Sil; Lee, Eun Jung; Cho, Jaeho.

In: Biochemical and Biophysical Research Communications, Vol. 460, No. 3, 02.05.2015, p. 504-510.

Research output: Contribution to journalArticle

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T2 - A therapeutic opportunity in radiation-induced skin injury

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AU - Kang, Jeong Wook

AU - Lee, Dong Won

AU - Oh, SangHo

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AU - Lee, Eun Jung

AU - Cho, Jaeho

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