Ionizing radiation is used to treat a range of cancers. Despite recent technological progress, radiation therapy can damage the skin at the administration site. The specific molecular mechanisms involved in this effect have not been fully characterized. In this study, the effects of pyruvate, on radiation-induced skin injury were investigated, including the role of the pyruvate dehydrogenase kinase 2 (PDK2) signaling pathway. Next generation sequencing (NGS) identified a wide range of gene expression differences between the control and irradiated mice, including reduced expression of PDK2. This was confirmed using Q-PCR. Cell culture studies demonstrated that PDK2 overexpression and a high cellular pyruvate concentration inhibited radiation-induced cytokine expression. Immunohistochemical studies demonstrated radiation-induced skin thickening and gene expression changes. Oral pyruvate treatment markedly downregulated radiation-induced changes in skin thickness and inflammatory cytokine expression. These findings indicated that regulation of the pyruvate metabolic pathway could provide an effective approach to the control of radiation-induced skin damage.
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 2015 May 2|
Bibliographical noteFunding Information:
This work was supported by the Nuclear Research and Development Program ( NRF-2011-0031695 ) and by the Radiation Technology Research and Development Program ( NRF-2013M2A2A7042978 ) through the National Research Foundation of Korea, funded by the Ministry of Science, ICT & Future Planning as well as by a faculty research grant from Yonsei University College of Medicine for 2014 ( 6-2014-0031 ).
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology