Quality control of Pulsatilla koreana based on the simultaneous determination of triterpenoidal saponins by HPLC-ELSD and principal component analysis

Ki Yong Lee, Young Woong Cho, Junghyun Park, Dong Young Lee, Seung Hyun Kim, Young Choong Kim, Sang Hyun Sung

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Introduction - Pulsatilla koreana Nakai, with triterpenoidal saponins as its main pharmacological effective compounds, is known to have several biological activities, including hypoglycaemic, antitumour, cognition-enhancing, neuroprotective, cytotoxic and antiangiogenic activities. However, few analytical methods have been reported on the quality assessment of P. koreana roots. Obejective - To establish a high-performance liquid chromatography coupled with evaporative light scattering detection for the simultaneous determination of five triterpenoidal saponins, including pulsatilloside E (1), pulsatilla saponin H (2), anemoside B 4 (3), hederacolchiside E (4) and cussosaponin C (5) in P. koreana. Methodology - The chromatographic separation was performed on a Shiseido CapCell PAK C 18 analytical column efficiently using gradient elution with acetonitrile and water. Results - All calibration curves showed excellent linear regressions (R 2 < 0.9996) within the range of tested concentrations. The intra- and inter-day variations were below 4.78% in terms of RSD. The recoveries were 94.82-102.97% with RSD of 0.27-3.92% for spiked P. koreana samples. Conclusion - The validated method was successfully used for the analysis of five saponins in P. koreana from different locations. Moreover, the different samples were clustered in accordance with contents of triterpenoidal saponins based on aglycon type by a principal component analysis.

Original languageEnglish
Pages (from-to)314-321
Number of pages8
JournalPhytochemical Analysis
Volume21
Issue number4
DOIs
Publication statusPublished - 2010 Jul 1

Fingerprint

Anemone cernua
Pulsatilla
Saponins
Principal Component Analysis
saponins
Quality Control
Principal component analysis
quality control
Quality control
principal component analysis
high performance liquid chromatography
High Pressure Liquid Chromatography
High performance liquid chromatography
light scattering
Bioactivity
Hypoglycemic Agents
Linear regression
cognition
Light scattering
Cognition

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science
  • Biochemistry
  • Molecular Medicine
  • Plant Science
  • Drug Discovery
  • Complementary and alternative medicine

Cite this

Lee, Ki Yong ; Cho, Young Woong ; Park, Junghyun ; Lee, Dong Young ; Kim, Seung Hyun ; Kim, Young Choong ; Sung, Sang Hyun. / Quality control of Pulsatilla koreana based on the simultaneous determination of triterpenoidal saponins by HPLC-ELSD and principal component analysis. In: Phytochemical Analysis. 2010 ; Vol. 21, No. 4. pp. 314-321.
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abstract = "Introduction - Pulsatilla koreana Nakai, with triterpenoidal saponins as its main pharmacological effective compounds, is known to have several biological activities, including hypoglycaemic, antitumour, cognition-enhancing, neuroprotective, cytotoxic and antiangiogenic activities. However, few analytical methods have been reported on the quality assessment of P. koreana roots. Obejective - To establish a high-performance liquid chromatography coupled with evaporative light scattering detection for the simultaneous determination of five triterpenoidal saponins, including pulsatilloside E (1), pulsatilla saponin H (2), anemoside B 4 (3), hederacolchiside E (4) and cussosaponin C (5) in P. koreana. Methodology - The chromatographic separation was performed on a Shiseido CapCell PAK C 18 analytical column efficiently using gradient elution with acetonitrile and water. Results - All calibration curves showed excellent linear regressions (R 2 < 0.9996) within the range of tested concentrations. The intra- and inter-day variations were below 4.78{\%} in terms of RSD. The recoveries were 94.82-102.97{\%} with RSD of 0.27-3.92{\%} for spiked P. koreana samples. Conclusion - The validated method was successfully used for the analysis of five saponins in P. koreana from different locations. Moreover, the different samples were clustered in accordance with contents of triterpenoidal saponins based on aglycon type by a principal component analysis.",
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Quality control of Pulsatilla koreana based on the simultaneous determination of triterpenoidal saponins by HPLC-ELSD and principal component analysis. / Lee, Ki Yong; Cho, Young Woong; Park, Junghyun; Lee, Dong Young; Kim, Seung Hyun; Kim, Young Choong; Sung, Sang Hyun.

In: Phytochemical Analysis, Vol. 21, No. 4, 01.07.2010, p. 314-321.

Research output: Contribution to journalArticle

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AU - Lee, Ki Yong

AU - Cho, Young Woong

AU - Park, Junghyun

AU - Lee, Dong Young

AU - Kim, Seung Hyun

AU - Kim, Young Choong

AU - Sung, Sang Hyun

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N2 - Introduction - Pulsatilla koreana Nakai, with triterpenoidal saponins as its main pharmacological effective compounds, is known to have several biological activities, including hypoglycaemic, antitumour, cognition-enhancing, neuroprotective, cytotoxic and antiangiogenic activities. However, few analytical methods have been reported on the quality assessment of P. koreana roots. Obejective - To establish a high-performance liquid chromatography coupled with evaporative light scattering detection for the simultaneous determination of five triterpenoidal saponins, including pulsatilloside E (1), pulsatilla saponin H (2), anemoside B 4 (3), hederacolchiside E (4) and cussosaponin C (5) in P. koreana. Methodology - The chromatographic separation was performed on a Shiseido CapCell PAK C 18 analytical column efficiently using gradient elution with acetonitrile and water. Results - All calibration curves showed excellent linear regressions (R 2 < 0.9996) within the range of tested concentrations. The intra- and inter-day variations were below 4.78% in terms of RSD. The recoveries were 94.82-102.97% with RSD of 0.27-3.92% for spiked P. koreana samples. Conclusion - The validated method was successfully used for the analysis of five saponins in P. koreana from different locations. Moreover, the different samples were clustered in accordance with contents of triterpenoidal saponins based on aglycon type by a principal component analysis.

AB - Introduction - Pulsatilla koreana Nakai, with triterpenoidal saponins as its main pharmacological effective compounds, is known to have several biological activities, including hypoglycaemic, antitumour, cognition-enhancing, neuroprotective, cytotoxic and antiangiogenic activities. However, few analytical methods have been reported on the quality assessment of P. koreana roots. Obejective - To establish a high-performance liquid chromatography coupled with evaporative light scattering detection for the simultaneous determination of five triterpenoidal saponins, including pulsatilloside E (1), pulsatilla saponin H (2), anemoside B 4 (3), hederacolchiside E (4) and cussosaponin C (5) in P. koreana. Methodology - The chromatographic separation was performed on a Shiseido CapCell PAK C 18 analytical column efficiently using gradient elution with acetonitrile and water. Results - All calibration curves showed excellent linear regressions (R 2 < 0.9996) within the range of tested concentrations. The intra- and inter-day variations were below 4.78% in terms of RSD. The recoveries were 94.82-102.97% with RSD of 0.27-3.92% for spiked P. koreana samples. Conclusion - The validated method was successfully used for the analysis of five saponins in P. koreana from different locations. Moreover, the different samples were clustered in accordance with contents of triterpenoidal saponins based on aglycon type by a principal component analysis.

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