Quantification of cellular properties from external fields and resulting induced velocity: Cellular hydrodynamic diameter

Jeffrey J. Chalmers, Seungjoo Haam, Yang Zhao, Kara McCloskey, Lee Moore, Maciej Zborowski, P. Stephen Williams

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

An experimental technique is discussed in which the size distribution of a population of cells is determined by calculating each cell's settling velocity. The settling velocity is determined from microscopically obtained images which were recorded on SVHS tape. These images are then computer imaged and processed, and the cell's location and velocity are determined using a computer algorithm referred to as cell tracking velocimetry (CTV). Experimental data is presented comparing the distribution of human lymphocytes and a human breast cancer cell line, MCF-7, determined using a Coulter counter and the CTV approach.

Original languageEnglish
Pages (from-to)509-518
Number of pages10
JournalBiotechnology and Bioengineering
Volume64
Issue number5
DOIs
Publication statusPublished - 1999 Mar 20

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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