Quantification of cellular uptake and in vivo tracking of transduction using real-time monitoring

Mi Sook Lee; Eun Hye Kwon; Hoon Sung Choi; Seung Hae Kwon; Chul Hyun Lee; In sop Shim; Sang Kyou Lee; Song Her

doi:10.1016/j.bbrc.2010.03.009

Quantification of cellular uptake and in vivo tracking of transduction using real-time monitoring

Mi Sook Lee, Eun Hye Kwon, Hoon Sung Choi, Seung Hae Kwon, Chul Hyun Lee, In sop Shim, Sang Kyou Lee, Song Her

Research output: Contribution to journal › Article

19 Citations (Scopus)

Abstract

Protein transduction domains (PTDs) are short amino acid sequences that promote their own translocation across the cell plasma membrane and have been studied for possible use in drug delivery and gene therapy. However, no direct method to quantify transduction is available. Here, using a new luciferase-tagged human PTD, we show that cellular uptake levels can be determined in a reliable manner. Furthermore, we show that enhanced in vivo tracking by human PTD can be quantified in a mouse model. This is the first report on the direct quantification of PTD transduction in vitro and in vivo, which will be necessary for studying its possible therapeutic application in drug delivery and gene therapy.

Original language	English
Pages (from-to)	348-353
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	394
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2010.03.009
Publication status	Published - 2010 Apr 2

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Lee, M. S., Kwon, E. H., Choi, H. S., Kwon, S. H., Lee, C. H., Shim, I. S., Lee, S. K., & Her, S. (2010). Quantification of cellular uptake and in vivo tracking of transduction using real-time monitoring. Biochemical and Biophysical Research Communications, 394(2), 348-353. https://doi.org/10.1016/j.bbrc.2010.03.009

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Quantification of cellular uptake and in vivo tracking of transduction using real-time monitoring. / Lee, Mi Sook; Kwon, Eun Hye; Choi, Hoon Sung; Kwon, Seung Hae; Lee, Chul Hyun; Shim, In sop; Lee, Sang Kyou; Her, Song.

In: Biochemical and Biophysical Research Communications, Vol. 394, No. 2, 02.04.2010, p. 348-353.

Research output: Contribution to journal › Article

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