Protein transduction domains (PTDs) are short amino acid sequences that promote their own translocation across the cell plasma membrane and have been studied for possible use in drug delivery and gene therapy. However, no direct method to quantify transduction is available. Here, using a new luciferase-tagged human PTD, we show that cellular uptake levels can be determined in a reliable manner. Furthermore, we show that enhanced in vivo tracking by human PTD can be quantified in a mouse model. This is the first report on the direct quantification of PTD transduction in vitro and in vivo, which will be necessary for studying its possible therapeutic application in drug delivery and gene therapy.
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 2010 Apr 2|
Bibliographical noteFunding Information:
This study was supported by a grant from the Small Business Administration, Republic of Korea ( PB8010 ), and by a grant from the Korean Basic Science Institute ( T29740 ).
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology