Quantification of cellular uptake and in vivo tracking of transduction using real-time monitoring

Mi Sook Lee, Eun Hye Kwon, Hoon Sung Choi, Seung Hae Kwon, Chul Hyun Lee, In sop Shim, Sang Kyou Lee, Song Her

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Protein transduction domains (PTDs) are short amino acid sequences that promote their own translocation across the cell plasma membrane and have been studied for possible use in drug delivery and gene therapy. However, no direct method to quantify transduction is available. Here, using a new luciferase-tagged human PTD, we show that cellular uptake levels can be determined in a reliable manner. Furthermore, we show that enhanced in vivo tracking by human PTD can be quantified in a mouse model. This is the first report on the direct quantification of PTD transduction in vitro and in vivo, which will be necessary for studying its possible therapeutic application in drug delivery and gene therapy.

Original languageEnglish
Pages (from-to)348-353
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume394
Issue number2
DOIs
Publication statusPublished - 2010 Apr 2

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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