Quantification of cellular uptake and in vivo tracking of transduction using real-time monitoring

Mi Sook Lee; Eun Hye Kwon; Hoon Sung Choi; Seung Hae Kwon; Chul Hyun Lee; In sop Shim; Sang Kyou Lee; Song Her

doi:10.1016/j.bbrc.2010.03.009

Quantification of cellular uptake and in vivo tracking of transduction using real-time monitoring

Mi Sook Lee, Eun Hye Kwon, Hoon Sung Choi, Seung Hae Kwon, Chul Hyun Lee, In sop Shim, Sang Kyou Lee, Song Her

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Protein transduction domains (PTDs) are short amino acid sequences that promote their own translocation across the cell plasma membrane and have been studied for possible use in drug delivery and gene therapy. However, no direct method to quantify transduction is available. Here, using a new luciferase-tagged human PTD, we show that cellular uptake levels can be determined in a reliable manner. Furthermore, we show that enhanced in vivo tracking by human PTD can be quantified in a mouse model. This is the first report on the direct quantification of PTD transduction in vitro and in vivo, which will be necessary for studying its possible therapeutic application in drug delivery and gene therapy.

Original language	English
Pages (from-to)	348-353
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	394
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2010.03.009
Publication status	Published - 2010 Apr 2

Bibliographical note

Funding Information:
This study was supported by a grant from the Small Business Administration, Republic of Korea ( PB8010 ), and by a grant from the Korean Basic Science Institute ( T29740 ).

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2010.03.009

Cite this

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title = "Quantification of cellular uptake and in vivo tracking of transduction using real-time monitoring",

abstract = "Protein transduction domains (PTDs) are short amino acid sequences that promote their own translocation across the cell plasma membrane and have been studied for possible use in drug delivery and gene therapy. However, no direct method to quantify transduction is available. Here, using a new luciferase-tagged human PTD, we show that cellular uptake levels can be determined in a reliable manner. Furthermore, we show that enhanced in vivo tracking by human PTD can be quantified in a mouse model. This is the first report on the direct quantification of PTD transduction in vitro and in vivo, which will be necessary for studying its possible therapeutic application in drug delivery and gene therapy.",

author = "Lee, {Mi Sook} and Kwon, {Eun Hye} and Choi, {Hoon Sung} and Kwon, {Seung Hae} and Lee, {Chul Hyun} and Shim, {In sop} and Lee, {Sang Kyou} and Song Her",

note = "Funding Information: This study was supported by a grant from the Small Business Administration, Republic of Korea ( PB8010 ), and by a grant from the Korean Basic Science Institute ( T29740 ). ",

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doi = "10.1016/j.bbrc.2010.03.009",

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AU - Lee, Mi Sook

AU - Kwon, Eun Hye

AU - Choi, Hoon Sung

AU - Kwon, Seung Hae

AU - Lee, Chul Hyun

AU - Shim, In sop

AU - Lee, Sang Kyou

AU - Her, Song

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PY - 2010/4/2

Y1 - 2010/4/2

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AB - Protein transduction domains (PTDs) are short amino acid sequences that promote their own translocation across the cell plasma membrane and have been studied for possible use in drug delivery and gene therapy. However, no direct method to quantify transduction is available. Here, using a new luciferase-tagged human PTD, we show that cellular uptake levels can be determined in a reliable manner. Furthermore, we show that enhanced in vivo tracking by human PTD can be quantified in a mouse model. This is the first report on the direct quantification of PTD transduction in vitro and in vivo, which will be necessary for studying its possible therapeutic application in drug delivery and gene therapy.

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Quantification of cellular uptake and in vivo tracking of transduction using real-time monitoring

Abstract

Bibliographical note

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