Quantification of protein-ligand interaction using supported lipid bilayer assisted biosensors

Donggeun Lee, Youngmo Jung, Taikjin Lee, Jae Hun Kim, Seok Lee, Sang Kook Han, Yong Sang Ryu, Chulki Kim

Research output: Contribution to journalConference article

Abstract

Quantification of kinetics of protein interactions has been a fundamental challenge in biophysics and biotechnology [1],[2]. To investigate the binding kinetics on a cell membrane rigorously, active ligands should be prepared in a controlled environment in terms of the number of binding sites and its kind. Conventional binding assays using ligand immobilization techniques with glue-like layers still have problems typically related to ligand denaturalization and non-specific binding. To demonstrate monitoring real-time binding kinetics between proteins and ligands, we introduce a supported lipid bilayer (SLB) to model the binding kinetics. The role of the supported lipid bilayer here is three-fold: accurate control over the binding sites, structural formation of receptors, and reducing non-specific bindings effectively. We adopted a field effective transistor device capable of reliable observation of protein interactions via its modulated current responses. The binding sites and rate constants of the protein-ligand pair interaction are determined by monitoring the real-time reaction kinetics, demonstrating the possible quantification of protein interactions with a detection limit of picomolar concentration and association constant was about 1 × 109 M-1 using SLB assisted biosensors.

Original languageEnglish
Article numberICNFA 119
JournalProceedings of the World Congress on New Technologies
DOIs
Publication statusPublished - 2018 Jan 1
Event4th World Congress on New Technologies, NEWTECH 2018 - Madrid, Spain
Duration: 2018 Aug 192018 Aug 21

Fingerprint

Lipid bilayers
Lipid Bilayers
Biosensing Techniques
Biosensors
ligand
lipid
Ligands
Proteins
Binding sites
protein
kinetics
Kinetics
Binding Sites
biophysics
Biophysics
Controlled Environment
Glues
Monitoring
reaction kinetics
Cell membranes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Energy Engineering and Power Technology
  • Biomedical Engineering
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Management, Monitoring, Policy and Law
  • Pollution
  • Electronic, Optical and Magnetic Materials

Cite this

Lee, Donggeun ; Jung, Youngmo ; Lee, Taikjin ; Kim, Jae Hun ; Lee, Seok ; Han, Sang Kook ; Ryu, Yong Sang ; Kim, Chulki. / Quantification of protein-ligand interaction using supported lipid bilayer assisted biosensors. In: Proceedings of the World Congress on New Technologies. 2018.
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Quantification of protein-ligand interaction using supported lipid bilayer assisted biosensors. / Lee, Donggeun; Jung, Youngmo; Lee, Taikjin; Kim, Jae Hun; Lee, Seok; Han, Sang Kook; Ryu, Yong Sang; Kim, Chulki.

In: Proceedings of the World Congress on New Technologies, 01.01.2018.

Research output: Contribution to journalConference article

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AU - Lee, Donggeun

AU - Jung, Youngmo

AU - Lee, Taikjin

AU - Kim, Jae Hun

AU - Lee, Seok

AU - Han, Sang Kook

AU - Ryu, Yong Sang

AU - Kim, Chulki

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