Quantification of kinetics of protein interactions has been a fundamental challenge in biophysics and biotechnology ,. To investigate the binding kinetics on a cell membrane rigorously, active ligands should be prepared in a controlled environment in terms of the number of binding sites and its kind. Conventional binding assays using ligand immobilization techniques with glue-like layers still have problems typically related to ligand denaturalization and non-specific binding. To demonstrate monitoring real-time binding kinetics between proteins and ligands, we introduce a supported lipid bilayer (SLB) to model the binding kinetics. The role of the supported lipid bilayer here is three-fold: accurate control over the binding sites, structural formation of receptors, and reducing non-specific bindings effectively. We adopted a field effective transistor device capable of reliable observation of protein interactions via its modulated current responses. The binding sites and rate constants of the protein-ligand pair interaction are determined by monitoring the real-time reaction kinetics, demonstrating the possible quantification of protein interactions with a detection limit of picomolar concentration and association constant was about 1 × 109 M-1 using SLB assisted biosensors.
|Article number||ICNFA 119|
|Journal||Proceedings of the World Congress on New Technologies|
|Publication status||Published - 2018|
|Event||4th World Congress on New Technologies, NEWTECH 2018 - Madrid, Spain|
Duration: 2018 Aug 19 → 2018 Aug 21
Bibliographical notePublisher Copyright:
© 2018, Avestia Publishing.
All Science Journal Classification (ASJC) codes
- Energy Engineering and Power Technology
- Biomedical Engineering
- Electrical and Electronic Engineering
- Mechanical Engineering
- Management, Monitoring, Policy and Law
- Electronic, Optical and Magnetic Materials