Quantification of protein-ligand interaction using supported lipid bilayer assisted biosensors

Donggeun Lee, Youngmo Jung, Taikjin Lee, Jae Hun Kim, Seok Lee, Sang Kook Han, Yong Sang Ryu, Chulki Kim

Research output: Contribution to journalConference articlepeer-review

Abstract

Quantification of kinetics of protein interactions has been a fundamental challenge in biophysics and biotechnology [1],[2]. To investigate the binding kinetics on a cell membrane rigorously, active ligands should be prepared in a controlled environment in terms of the number of binding sites and its kind. Conventional binding assays using ligand immobilization techniques with glue-like layers still have problems typically related to ligand denaturalization and non-specific binding. To demonstrate monitoring real-time binding kinetics between proteins and ligands, we introduce a supported lipid bilayer (SLB) to model the binding kinetics. The role of the supported lipid bilayer here is three-fold: accurate control over the binding sites, structural formation of receptors, and reducing non-specific bindings effectively. We adopted a field effective transistor device capable of reliable observation of protein interactions via its modulated current responses. The binding sites and rate constants of the protein-ligand pair interaction are determined by monitoring the real-time reaction kinetics, demonstrating the possible quantification of protein interactions with a detection limit of picomolar concentration and association constant was about 1 × 109 M-1 using SLB assisted biosensors.

Original languageEnglish
Article numberICNFA 119
JournalProceedings of the World Congress on New Technologies
DOIs
Publication statusPublished - 2018
Event4th World Congress on New Technologies, NEWTECH 2018 - Madrid, Spain
Duration: 2018 Aug 192018 Aug 21

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Energy Engineering and Power Technology
  • Biomedical Engineering
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Management, Monitoring, Policy and Law
  • Pollution
  • Electronic, Optical and Magnetic Materials

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