Quantitation of α-galactosides in Rehmannia glutinosa by hydrophilic interaction chromatography-evaporative light scattering detector

Tae Bum Kim, Seung Hyun Kim, Sang Hyun Sung

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Introduction The use of raffinose family oligosaccharides (RFOs) to control several diseases and stimulate immune systems has been studied. Rehmannia glutinosa is reported to contain RFOs; however, there are no reports on their content level in R. glutinosa. Furthermore, it is difficult to analyse RFOs using common reverse-phase chromatographic techniques due to their high polarity. Objective To develop a rapid and reliable chromatographic method for the simultaneous quantitation of multiple RFOs in R. glutinosa. Methodology Chromatographic separation was performed by using hydrophilic interaction liquid chromatography coupled with an evaporative light scattering detector. For efficient ultrasound-assisted extraction of oligosaccharides, a central composite design was used. Results Response surface analysis estimated the highest-obtained yields of raffinose, stachyose and verbascose to be 58.31, 408.75 and 26.59 mg, respectively, from 1 g of R. glutinosa, while under optimal conditions, experimental yields per gram were 56.79, 391.42 and 27.69 mg, respectively. Conclusion A rapid and reliable chromatographic method to simultaneously quantitate multiple RFOs in R. glutinosa was developed. Three RFOs in R. glutinosa were separated within 10 min using isocratic conditions. Quantitative analysis of RFOs showed that R. glutinosa may be a rich source of α-galacto-oligosaccharides, especially stachyose.

Original languageEnglish
Pages (from-to)607-612
Number of pages6
JournalPhytochemical Analysis
Volume23
Issue number6
DOIs
Publication statusPublished - 2012 Nov 1

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Rehmannia
Rehmannia glutinosa
Galactosides
Raffinose
galactosides
raffinose
light scattering
Chromatography
Oligosaccharides
Hydrophobic and Hydrophilic Interactions
oligosaccharides
Light scattering
detectors
Detectors
Light
stachyose
galactooligosaccharides
Disease control
hydrophilic interaction chromatography
Immune system

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science
  • Biochemistry
  • Molecular Medicine
  • Plant Science
  • Drug Discovery
  • Complementary and alternative medicine

Cite this

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abstract = "Introduction The use of raffinose family oligosaccharides (RFOs) to control several diseases and stimulate immune systems has been studied. Rehmannia glutinosa is reported to contain RFOs; however, there are no reports on their content level in R. glutinosa. Furthermore, it is difficult to analyse RFOs using common reverse-phase chromatographic techniques due to their high polarity. Objective To develop a rapid and reliable chromatographic method for the simultaneous quantitation of multiple RFOs in R. glutinosa. Methodology Chromatographic separation was performed by using hydrophilic interaction liquid chromatography coupled with an evaporative light scattering detector. For efficient ultrasound-assisted extraction of oligosaccharides, a central composite design was used. Results Response surface analysis estimated the highest-obtained yields of raffinose, stachyose and verbascose to be 58.31, 408.75 and 26.59 mg, respectively, from 1 g of R. glutinosa, while under optimal conditions, experimental yields per gram were 56.79, 391.42 and 27.69 mg, respectively. Conclusion A rapid and reliable chromatographic method to simultaneously quantitate multiple RFOs in R. glutinosa was developed. Three RFOs in R. glutinosa were separated within 10 min using isocratic conditions. Quantitative analysis of RFOs showed that R. glutinosa may be a rich source of α-galacto-oligosaccharides, especially stachyose.",
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Quantitation of α-galactosides in Rehmannia glutinosa by hydrophilic interaction chromatography-evaporative light scattering detector. / Kim, Tae Bum; Kim, Seung Hyun; Sung, Sang Hyun.

In: Phytochemical Analysis, Vol. 23, No. 6, 01.11.2012, p. 607-612.

Research output: Contribution to journalArticle

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AB - Introduction The use of raffinose family oligosaccharides (RFOs) to control several diseases and stimulate immune systems has been studied. Rehmannia glutinosa is reported to contain RFOs; however, there are no reports on their content level in R. glutinosa. Furthermore, it is difficult to analyse RFOs using common reverse-phase chromatographic techniques due to their high polarity. Objective To develop a rapid and reliable chromatographic method for the simultaneous quantitation of multiple RFOs in R. glutinosa. Methodology Chromatographic separation was performed by using hydrophilic interaction liquid chromatography coupled with an evaporative light scattering detector. For efficient ultrasound-assisted extraction of oligosaccharides, a central composite design was used. Results Response surface analysis estimated the highest-obtained yields of raffinose, stachyose and verbascose to be 58.31, 408.75 and 26.59 mg, respectively, from 1 g of R. glutinosa, while under optimal conditions, experimental yields per gram were 56.79, 391.42 and 27.69 mg, respectively. Conclusion A rapid and reliable chromatographic method to simultaneously quantitate multiple RFOs in R. glutinosa was developed. Three RFOs in R. glutinosa were separated within 10 min using isocratic conditions. Quantitative analysis of RFOs showed that R. glutinosa may be a rich source of α-galacto-oligosaccharides, especially stachyose.

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