Quantitative analysis of BMP-2 derived peptide covalently grafted onto oxidized detonation nanodiamonds

Sascha Balakin, Athanassios Missirlis, Benjamin Klemmed, Jihye Lee, Jörg Opitz, Jong Souk Yeo, Gianaurelio Cuniberti

Research output: Contribution to journalArticle

Abstract

In order to deploy detonation nanodiamonds (NDs) in nanomedicine and drug delivery applications, fundamental understanding of their surface chemistry and drug loading capacity is highly desirable. Herein, bone morphogenetic protein 2 (BMP-2) derived peptide with the sequence KIPKASSVPTELSAISTLYLGGC (molecular weight = 2336 g/mol) have been successfully grafted onto NDs using carbodiimide crosslinker chemistry. Initially, functional surface groups of wet- and dry-oxidized NDs were compared using infrared and mass spectroscopy. Dry-oxidized NDs exhibited the highest amount of carboxylic acid derivatives with a surface loading of 0.113 mmol/g after air annealing at 415 °C for 5 h as determined by mass spectroscopy. Compared to wet-oxidation, the dry-oxidation process showed a 1.4-fold increased amount of carbonyl compounds deduced from infrared and mass spectroscopy. Crosslinking of the carboxylic acid derivatives to the primary amines of BMP-2 derived peptide is feasible in the range of 30–96% total surface coverage of NDs. 1:1 and 5:1 ND-peptide ratios were utilized to study the surface loading of NDs using fluorescence spectroscopy. The chemisorption of BMP-2 derived peptide onto NDs reveals superior surface coverage and results in reproducible amounts of tethered bioactive molecules.

Original languageEnglish
Pages (from-to)740-745
Number of pages6
JournalCarbon
Volume152
DOIs
Publication statusPublished - 2019 Nov

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)

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