Abstract
In order to deploy detonation nanodiamonds (NDs) in nanomedicine and drug delivery applications, fundamental understanding of their surface chemistry and drug loading capacity is highly desirable. Herein, bone morphogenetic protein 2 (BMP-2) derived peptide with the sequence KIPKASSVPTELSAISTLYLGGC (molecular weight = 2336 g/mol) have been successfully grafted onto NDs using carbodiimide crosslinker chemistry. Initially, functional surface groups of wet- and dry-oxidized NDs were compared using infrared and mass spectroscopy. Dry-oxidized NDs exhibited the highest amount of carboxylic acid derivatives with a surface loading of 0.113 mmol/g after air annealing at 415 °C for 5 h as determined by mass spectroscopy. Compared to wet-oxidation, the dry-oxidation process showed a 1.4-fold increased amount of carbonyl compounds deduced from infrared and mass spectroscopy. Crosslinking of the carboxylic acid derivatives to the primary amines of BMP-2 derived peptide is feasible in the range of 30–96% total surface coverage of NDs. 1:1 and 5:1 ND-peptide ratios were utilized to study the surface loading of NDs using fluorescence spectroscopy. The chemisorption of BMP-2 derived peptide onto NDs reveals superior surface coverage and results in reproducible amounts of tethered bioactive molecules.
| Original language | English |
|---|---|
| Pages (from-to) | 740-745 |
| Number of pages | 6 |
| Journal | Carbon |
| Volume | 152 |
| DOIs | |
| Publication status | Published - 2019 Nov 1 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Materials Science(all)
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Quantitative analysis of BMP-2 derived peptide covalently grafted onto oxidized detonation nanodiamonds. / Balakin, Sascha; Missirlis, Athanassios; Klemmed, Benjamin; Lee, Jihye; Opitz, Jörg; Yeo, Jong-Souk; Cuniberti, Gianaurelio.
In: Carbon, Vol. 152, 01.11.2019, p. 740-745.Research output: Contribution to journal › Article
TY - JOUR
T1 - Quantitative analysis of BMP-2 derived peptide covalently grafted onto oxidized detonation nanodiamonds
AU - Balakin, Sascha
AU - Missirlis, Athanassios
AU - Klemmed, Benjamin
AU - Lee, Jihye
AU - Opitz, Jörg
AU - Yeo, Jong-Souk
AU - Cuniberti, Gianaurelio
PY - 2019/11/1
Y1 - 2019/11/1
N2 - In order to deploy detonation nanodiamonds (NDs) in nanomedicine and drug delivery applications, fundamental understanding of their surface chemistry and drug loading capacity is highly desirable. Herein, bone morphogenetic protein 2 (BMP-2) derived peptide with the sequence KIPKASSVPTELSAISTLYLGGC (molecular weight = 2336 g/mol) have been successfully grafted onto NDs using carbodiimide crosslinker chemistry. Initially, functional surface groups of wet- and dry-oxidized NDs were compared using infrared and mass spectroscopy. Dry-oxidized NDs exhibited the highest amount of carboxylic acid derivatives with a surface loading of 0.113 mmol/g after air annealing at 415 °C for 5 h as determined by mass spectroscopy. Compared to wet-oxidation, the dry-oxidation process showed a 1.4-fold increased amount of carbonyl compounds deduced from infrared and mass spectroscopy. Crosslinking of the carboxylic acid derivatives to the primary amines of BMP-2 derived peptide is feasible in the range of 30–96% total surface coverage of NDs. 1:1 and 5:1 ND-peptide ratios were utilized to study the surface loading of NDs using fluorescence spectroscopy. The chemisorption of BMP-2 derived peptide onto NDs reveals superior surface coverage and results in reproducible amounts of tethered bioactive molecules.
AB - In order to deploy detonation nanodiamonds (NDs) in nanomedicine and drug delivery applications, fundamental understanding of their surface chemistry and drug loading capacity is highly desirable. Herein, bone morphogenetic protein 2 (BMP-2) derived peptide with the sequence KIPKASSVPTELSAISTLYLGGC (molecular weight = 2336 g/mol) have been successfully grafted onto NDs using carbodiimide crosslinker chemistry. Initially, functional surface groups of wet- and dry-oxidized NDs were compared using infrared and mass spectroscopy. Dry-oxidized NDs exhibited the highest amount of carboxylic acid derivatives with a surface loading of 0.113 mmol/g after air annealing at 415 °C for 5 h as determined by mass spectroscopy. Compared to wet-oxidation, the dry-oxidation process showed a 1.4-fold increased amount of carbonyl compounds deduced from infrared and mass spectroscopy. Crosslinking of the carboxylic acid derivatives to the primary amines of BMP-2 derived peptide is feasible in the range of 30–96% total surface coverage of NDs. 1:1 and 5:1 ND-peptide ratios were utilized to study the surface loading of NDs using fluorescence spectroscopy. The chemisorption of BMP-2 derived peptide onto NDs reveals superior surface coverage and results in reproducible amounts of tethered bioactive molecules.
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U2 - 10.1016/j.carbon.2019.05.064
DO - 10.1016/j.carbon.2019.05.064
M3 - Article
VL - 152
SP - 740
EP - 745
JO - Carbon
JF - Carbon
SN - 0008-6223
ER -