Quantitative analysis of interleukin-6 expression in porcine spleen cells and alveolar macrophages using real-time PCR

D. Y. Lee, Y. W. Cho, S. G. Kang, N. R. Shin, I. S. Choi, SungJae Shin, H. S. Yoo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Interleukin-6 (IL-6), a multifocal cytokine produced by lymphoid and non-lymphoid cells, regulates immune responses, acute-phase reactions against bacterial infections, and haematopoiesis. After cloning and sequencing of porcine IL-6, the expression pattern of porcine IL-6 mRNA was evaluated through real-time RT-PCR using porcine immune cells (spleen cells and alveolar macrophages) following stimulation with LPS. The sequence has been reported to GenBank with Accession no. AF 518322. The nucleotide sequence was different at the 89th and 205th positions in comparison with M80258, but only at the 205th with M86722. Comparison of porcine IL-6, Accession no. AF 518322, with IL-6 of human, canine, ovine, and mouse showed homologies of 78%, 81%, 82% and 73% in nucleotide sequence and 42%, 69%, 61% and 42% in amino acids. Expression of IL-6 mRNA was induced by stimulation with LPS. IL-6 mRNA expression in alveolar macrophages peaked at 2 h and decreased sharply to control levels at 4 h, whereas it peaked at 14 h and decreased at 24 h in spleen cells after stimulation with LPS (1 μg/ml). These results suggest that IL-6 mRNA expression in porcine immune cells is cell-type specific and the results of this study could be used as the basis for research on the porcine immune system.

Original languageEnglish
Pages (from-to)503-513
Number of pages11
JournalVeterinary Research Communications
Volume28
Issue number6
DOIs
Publication statusPublished - 2004 Aug 1

Fingerprint

Alveolar Macrophages
splenocytes
interleukin-6
quantitative analysis
Real-Time Polymerase Chain Reaction
Interleukin-6
quantitative polymerase chain reaction
macrophages
Swine
Spleen
swine
Messenger RNA
cells
nucleotide sequences
Acute-Phase Reaction
hematopoiesis
Nucleic Acid Databases
Hematopoiesis
bacterial infections
Bacterial Infections

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Lee, D. Y. ; Cho, Y. W. ; Kang, S. G. ; Shin, N. R. ; Choi, I. S. ; Shin, SungJae ; Yoo, H. S. / Quantitative analysis of interleukin-6 expression in porcine spleen cells and alveolar macrophages using real-time PCR. In: Veterinary Research Communications. 2004 ; Vol. 28, No. 6. pp. 503-513.
@article{8cd12f0d9d6644ec8fe140a23c52163d,
title = "Quantitative analysis of interleukin-6 expression in porcine spleen cells and alveolar macrophages using real-time PCR",
abstract = "Interleukin-6 (IL-6), a multifocal cytokine produced by lymphoid and non-lymphoid cells, regulates immune responses, acute-phase reactions against bacterial infections, and haematopoiesis. After cloning and sequencing of porcine IL-6, the expression pattern of porcine IL-6 mRNA was evaluated through real-time RT-PCR using porcine immune cells (spleen cells and alveolar macrophages) following stimulation with LPS. The sequence has been reported to GenBank with Accession no. AF 518322. The nucleotide sequence was different at the 89th and 205th positions in comparison with M80258, but only at the 205th with M86722. Comparison of porcine IL-6, Accession no. AF 518322, with IL-6 of human, canine, ovine, and mouse showed homologies of 78{\%}, 81{\%}, 82{\%} and 73{\%} in nucleotide sequence and 42{\%}, 69{\%}, 61{\%} and 42{\%} in amino acids. Expression of IL-6 mRNA was induced by stimulation with LPS. IL-6 mRNA expression in alveolar macrophages peaked at 2 h and decreased sharply to control levels at 4 h, whereas it peaked at 14 h and decreased at 24 h in spleen cells after stimulation with LPS (1 μg/ml). These results suggest that IL-6 mRNA expression in porcine immune cells is cell-type specific and the results of this study could be used as the basis for research on the porcine immune system.",
author = "Lee, {D. Y.} and Cho, {Y. W.} and Kang, {S. G.} and Shin, {N. R.} and Choi, {I. S.} and SungJae Shin and Yoo, {H. S.}",
year = "2004",
month = "8",
day = "1",
doi = "10.1023/B:VERC.0000040242.85968.e0",
language = "English",
volume = "28",
pages = "503--513",
journal = "Veterinary Research Communications",
issn = "0165-7380",
publisher = "Springer Netherlands",
number = "6",

}

Quantitative analysis of interleukin-6 expression in porcine spleen cells and alveolar macrophages using real-time PCR. / Lee, D. Y.; Cho, Y. W.; Kang, S. G.; Shin, N. R.; Choi, I. S.; Shin, SungJae; Yoo, H. S.

In: Veterinary Research Communications, Vol. 28, No. 6, 01.08.2004, p. 503-513.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Quantitative analysis of interleukin-6 expression in porcine spleen cells and alveolar macrophages using real-time PCR

AU - Lee, D. Y.

AU - Cho, Y. W.

AU - Kang, S. G.

AU - Shin, N. R.

AU - Choi, I. S.

AU - Shin, SungJae

AU - Yoo, H. S.

PY - 2004/8/1

Y1 - 2004/8/1

N2 - Interleukin-6 (IL-6), a multifocal cytokine produced by lymphoid and non-lymphoid cells, regulates immune responses, acute-phase reactions against bacterial infections, and haematopoiesis. After cloning and sequencing of porcine IL-6, the expression pattern of porcine IL-6 mRNA was evaluated through real-time RT-PCR using porcine immune cells (spleen cells and alveolar macrophages) following stimulation with LPS. The sequence has been reported to GenBank with Accession no. AF 518322. The nucleotide sequence was different at the 89th and 205th positions in comparison with M80258, but only at the 205th with M86722. Comparison of porcine IL-6, Accession no. AF 518322, with IL-6 of human, canine, ovine, and mouse showed homologies of 78%, 81%, 82% and 73% in nucleotide sequence and 42%, 69%, 61% and 42% in amino acids. Expression of IL-6 mRNA was induced by stimulation with LPS. IL-6 mRNA expression in alveolar macrophages peaked at 2 h and decreased sharply to control levels at 4 h, whereas it peaked at 14 h and decreased at 24 h in spleen cells after stimulation with LPS (1 μg/ml). These results suggest that IL-6 mRNA expression in porcine immune cells is cell-type specific and the results of this study could be used as the basis for research on the porcine immune system.

AB - Interleukin-6 (IL-6), a multifocal cytokine produced by lymphoid and non-lymphoid cells, regulates immune responses, acute-phase reactions against bacterial infections, and haematopoiesis. After cloning and sequencing of porcine IL-6, the expression pattern of porcine IL-6 mRNA was evaluated through real-time RT-PCR using porcine immune cells (spleen cells and alveolar macrophages) following stimulation with LPS. The sequence has been reported to GenBank with Accession no. AF 518322. The nucleotide sequence was different at the 89th and 205th positions in comparison with M80258, but only at the 205th with M86722. Comparison of porcine IL-6, Accession no. AF 518322, with IL-6 of human, canine, ovine, and mouse showed homologies of 78%, 81%, 82% and 73% in nucleotide sequence and 42%, 69%, 61% and 42% in amino acids. Expression of IL-6 mRNA was induced by stimulation with LPS. IL-6 mRNA expression in alveolar macrophages peaked at 2 h and decreased sharply to control levels at 4 h, whereas it peaked at 14 h and decreased at 24 h in spleen cells after stimulation with LPS (1 μg/ml). These results suggest that IL-6 mRNA expression in porcine immune cells is cell-type specific and the results of this study could be used as the basis for research on the porcine immune system.

UR - http://www.scopus.com/inward/record.url?scp=4444235902&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4444235902&partnerID=8YFLogxK

U2 - 10.1023/B:VERC.0000040242.85968.e0

DO - 10.1023/B:VERC.0000040242.85968.e0

M3 - Article

VL - 28

SP - 503

EP - 513

JO - Veterinary Research Communications

JF - Veterinary Research Communications

SN - 0165-7380

IS - 6

ER -