Quantitative assessment of synovial vascularity using contrast-enhanced power doppler ultrasonography: Correlation with histologic findings and MR imaging findings in arthritic rabbit knee model

Sang Hoon Lee, Jin Suck Suh, Myung Jin Shin, Seong Moon Kim, Namkug Kim, Hyun Suh Sang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objective: To validate contrast-enhanced power Doppler ultrasonography (PD US) for the evaluation of synovial vascularity in an arthritic rabbit knee model in correlation with MR and histological findings. Materials and Methods: Power Doppler ultrasonography was performed for carrageenin-induced arthritic left knee and control right knee of 13 rabbits, first without and then with sonic contrast agent enhancement (Levovist, Schering, Berlin Germany), followed by gadolinium-enhanced MR imaging. Synovial vascularity was quantitatively assessed by calculating the color pixel area in power Doppler sonography using a computer-aided image analysis program and by grading the enhancement on MR images: grade 1, enhancement of knee joint is less than one-third of the area; grade 2, one-third to two-thirds enhancement; and grade 3, more than two-thirds enhancement. Microvessel density (MVD) was measured on slides stained immunohistochemically for CD31 antigen for histological assessment. Results: The mean area of color pixels in PD US changed from 4.37 to 16.42 mm2 in the arthritic knee after enhancement (p < 0.05), whereas it changed from 0.77 to 2.31 mm2 in the control knee (p < 0.05). Arthritic knees had greater power Doppler signal than control knees both before and after contrast administration (p < 0.05). The average MVD was 88 in arthritic knees and 46 in control knees. MVDs correlated with color pixel areas of contrast-enhanced power Doppler imaging in arthritic knees. In MR grading of arthritic knees, five were grade 2 and eight were grade 3. MVD and PD US revealed no significant difference between grade 2 and 3 arthritic knees (p > 0.05). Conclusion: Sonic contrast-enhanced PD US improves the visualization of synovial vascularity and allows quantitative measurement in experimentally induced rabbit arthritic knees.

Original languageEnglish
Pages (from-to)45-53
Number of pages9
JournalKorean journal of radiology
Volume9
Issue number1
DOIs
Publication statusPublished - 2008 Feb

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging

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