Quantitative detection of telomerase activity by real-time TRAP assay in the body fluids of cancer patients.

Woo Young Shim, Kyu Hyun Park, Hei Chul Jeung, Yong Tae Kim, Tae Soo Kim, Woo Jin Hyung, Sung Hwan An, Sang Hwa Yang, Sung Hoon Noh, Hyun Cheol Chung, Sun Young Rha

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Real-time TRAP assay was developed to improve the sensitivity and quantitative detection of telomerase activity in the body fluids of cancer patients. The sensitivity and clinical significance of the real-time TRAP assay was investigated. Real-time PCR protocol was modified by using ACX primer and SYBR green mixture from the process of TS primer extension. Real-time TRAP showed high correlation (r2=0.843, p=0.001) and sensitivity (25 times higher) compared to conventional TRAP. Of 164 samples, there were 8 positives in cytology (4.9%), 7 (4.3%) using the conventional TRAP, and 41 (25%) using real-time TRAP. In cytology positive samples, real-time TRAP showed a higher positivity than conventional TRAP (75% vs 63%) suggesting a higher sensitivity in the body fluids. There was a tendency towards a longer progression-free duration in telomerase negative patients than in positive patients, as determined by conventional and real-time TRAP. The diagnostic interval between the first positivity documentation and clinical progression was short in the order of real-time TRAP, conventional TRAP and cytology. In conclusion, real-time TRAP assay can detect telomerase activity at an earlier phase of cancer progression and can replace conventional TRAP assay for detecting the telomerase activity in body fluids.

Original languageEnglish
Pages (from-to)857-863
Number of pages7
JournalInternational journal of molecular medicine
Volume16
Issue number5
DOIs
Publication statusPublished - 2005 Nov

All Science Journal Classification (ASJC) codes

  • Genetics

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