Quantitative detection of telomerase activity by real-time TRAP assay in the body fluids of cancer patients.

Woo Young Shim, Kyu Hyun Park, Hei Chul Jeung, YoungTae Kim, Tae Soo Kim, WooJin Hyung, Sung Hwan An, Sang Hwa Yang, Sung Hoon Noh, Hyuncheol Chung, SunYoung Rha

Research output: Contribution to journalArticle

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Abstract

Real-time TRAP assay was developed to improve the sensitivity and quantitative detection of telomerase activity in the body fluids of cancer patients. The sensitivity and clinical significance of the real-time TRAP assay was investigated. Real-time PCR protocol was modified by using ACX primer and SYBR green mixture from the process of TS primer extension. Real-time TRAP showed high correlation (r2=0.843, p=0.001) and sensitivity (25 times higher) compared to conventional TRAP. Of 164 samples, there were 8 positives in cytology (4.9%), 7 (4.3%) using the conventional TRAP, and 41 (25%) using real-time TRAP. In cytology positive samples, real-time TRAP showed a higher positivity than conventional TRAP (75% vs 63%) suggesting a higher sensitivity in the body fluids. There was a tendency towards a longer progression-free duration in telomerase negative patients than in positive patients, as determined by conventional and real-time TRAP. The diagnostic interval between the first positivity documentation and clinical progression was short in the order of real-time TRAP, conventional TRAP and cytology. In conclusion, real-time TRAP assay can detect telomerase activity at an earlier phase of cancer progression and can replace conventional TRAP assay for detecting the telomerase activity in body fluids.

Original languageEnglish
Pages (from-to)857-863
Number of pages7
JournalInternational Journal of Molecular Medicine
Volume16
Issue number5
Publication statusPublished - 2005 Jan 1

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Telomerase
Body Fluids
Neoplasms
Cell Biology
Documentation
Real-Time Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Shim, Woo Young ; Park, Kyu Hyun ; Jeung, Hei Chul ; Kim, YoungTae ; Kim, Tae Soo ; Hyung, WooJin ; An, Sung Hwan ; Yang, Sang Hwa ; Noh, Sung Hoon ; Chung, Hyuncheol ; Rha, SunYoung. / Quantitative detection of telomerase activity by real-time TRAP assay in the body fluids of cancer patients. In: International Journal of Molecular Medicine. 2005 ; Vol. 16, No. 5. pp. 857-863.
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abstract = "Real-time TRAP assay was developed to improve the sensitivity and quantitative detection of telomerase activity in the body fluids of cancer patients. The sensitivity and clinical significance of the real-time TRAP assay was investigated. Real-time PCR protocol was modified by using ACX primer and SYBR green mixture from the process of TS primer extension. Real-time TRAP showed high correlation (r2=0.843, p=0.001) and sensitivity (25 times higher) compared to conventional TRAP. Of 164 samples, there were 8 positives in cytology (4.9{\%}), 7 (4.3{\%}) using the conventional TRAP, and 41 (25{\%}) using real-time TRAP. In cytology positive samples, real-time TRAP showed a higher positivity than conventional TRAP (75{\%} vs 63{\%}) suggesting a higher sensitivity in the body fluids. There was a tendency towards a longer progression-free duration in telomerase negative patients than in positive patients, as determined by conventional and real-time TRAP. The diagnostic interval between the first positivity documentation and clinical progression was short in the order of real-time TRAP, conventional TRAP and cytology. In conclusion, real-time TRAP assay can detect telomerase activity at an earlier phase of cancer progression and can replace conventional TRAP assay for detecting the telomerase activity in body fluids.",
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Quantitative detection of telomerase activity by real-time TRAP assay in the body fluids of cancer patients. / Shim, Woo Young; Park, Kyu Hyun; Jeung, Hei Chul; Kim, YoungTae; Kim, Tae Soo; Hyung, WooJin; An, Sung Hwan; Yang, Sang Hwa; Noh, Sung Hoon; Chung, Hyuncheol; Rha, SunYoung.

In: International Journal of Molecular Medicine, Vol. 16, No. 5, 01.01.2005, p. 857-863.

Research output: Contribution to journalArticle

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AU - Park, Kyu Hyun

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AU - Hyung, WooJin

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AU - Rha, SunYoung

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