Abstract
Real-time TRAP assay was developed to improve the sensitivity and quantitative detection of telomerase activity in the body fluids of cancer patients. The sensitivity and clinical significance of the real-time TRAP assay was investigated. Real-time PCR protocol was modified by using ACX primer and SYBR green mixture from the process of TS primer extension. Real-time TRAP showed high correlation (r2=0.843, p=0.001) and sensitivity (25 times higher) compared to conventional TRAP. Of 164 samples, there were 8 positives in cytology (4.9%), 7 (4.3%) using the conventional TRAP, and 41 (25%) using real-time TRAP. In cytology positive samples, real-time TRAP showed a higher positivity than conventional TRAP (75% vs 63%) suggesting a higher sensitivity in the body fluids. There was a tendency towards a longer progression-free duration in telomerase negative patients than in positive patients, as determined by conventional and real-time TRAP. The diagnostic interval between the first positivity documentation and clinical progression was short in the order of real-time TRAP, conventional TRAP and cytology. In conclusion, real-time TRAP assay can detect telomerase activity at an earlier phase of cancer progression and can replace conventional TRAP assay for detecting the telomerase activity in body fluids.
| Original language | English |
|---|---|
| Pages (from-to) | 857-863 |
| Number of pages | 7 |
| Journal | International Journal of Molecular Medicine |
| Volume | 16 |
| Issue number | 5 |
| Publication status | Published - 2005 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Genetics
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Quantitative detection of telomerase activity by real-time TRAP assay in the body fluids of cancer patients. / Shim, Woo Young; Park, Kyu Hyun; Jeung, Hei Chul; Kim, YoungTae; Kim, Tae Soo; Hyung, WooJin; An, Sung Hwan; Yang, Sang Hwa; Noh, Sung Hoon; Chung, Hyuncheol; Rha, SunYoung.
In: International Journal of Molecular Medicine, Vol. 16, No. 5, 01.01.2005, p. 857-863.Research output: Contribution to journal › Article
TY - JOUR
T1 - Quantitative detection of telomerase activity by real-time TRAP assay in the body fluids of cancer patients.
AU - Shim, Woo Young
AU - Park, Kyu Hyun
AU - Jeung, Hei Chul
AU - Kim, YoungTae
AU - Kim, Tae Soo
AU - Hyung, WooJin
AU - An, Sung Hwan
AU - Yang, Sang Hwa
AU - Noh, Sung Hoon
AU - Chung, Hyuncheol
AU - Rha, SunYoung
PY - 2005/1/1
Y1 - 2005/1/1
N2 - Real-time TRAP assay was developed to improve the sensitivity and quantitative detection of telomerase activity in the body fluids of cancer patients. The sensitivity and clinical significance of the real-time TRAP assay was investigated. Real-time PCR protocol was modified by using ACX primer and SYBR green mixture from the process of TS primer extension. Real-time TRAP showed high correlation (r2=0.843, p=0.001) and sensitivity (25 times higher) compared to conventional TRAP. Of 164 samples, there were 8 positives in cytology (4.9%), 7 (4.3%) using the conventional TRAP, and 41 (25%) using real-time TRAP. In cytology positive samples, real-time TRAP showed a higher positivity than conventional TRAP (75% vs 63%) suggesting a higher sensitivity in the body fluids. There was a tendency towards a longer progression-free duration in telomerase negative patients than in positive patients, as determined by conventional and real-time TRAP. The diagnostic interval between the first positivity documentation and clinical progression was short in the order of real-time TRAP, conventional TRAP and cytology. In conclusion, real-time TRAP assay can detect telomerase activity at an earlier phase of cancer progression and can replace conventional TRAP assay for detecting the telomerase activity in body fluids.
AB - Real-time TRAP assay was developed to improve the sensitivity and quantitative detection of telomerase activity in the body fluids of cancer patients. The sensitivity and clinical significance of the real-time TRAP assay was investigated. Real-time PCR protocol was modified by using ACX primer and SYBR green mixture from the process of TS primer extension. Real-time TRAP showed high correlation (r2=0.843, p=0.001) and sensitivity (25 times higher) compared to conventional TRAP. Of 164 samples, there were 8 positives in cytology (4.9%), 7 (4.3%) using the conventional TRAP, and 41 (25%) using real-time TRAP. In cytology positive samples, real-time TRAP showed a higher positivity than conventional TRAP (75% vs 63%) suggesting a higher sensitivity in the body fluids. There was a tendency towards a longer progression-free duration in telomerase negative patients than in positive patients, as determined by conventional and real-time TRAP. The diagnostic interval between the first positivity documentation and clinical progression was short in the order of real-time TRAP, conventional TRAP and cytology. In conclusion, real-time TRAP assay can detect telomerase activity at an earlier phase of cancer progression and can replace conventional TRAP assay for detecting the telomerase activity in body fluids.
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M3 - Article
C2 - 16211255
AN - SCOPUS:33644634016
VL - 16
SP - 857
EP - 863
JO - International Journal of Molecular Medicine
JF - International Journal of Molecular Medicine
SN - 1107-3756
IS - 5
ER -