Quantitative determination of sirolimus in dog blood using liquid chromatography-tandem mass spectrometry, and its applications to pharmacokinetic studies

Jong Hwa Lee, Kwang Ho Cha, Wonkyung Cho, Junsung Park, Hee Jun Park, Youngseok Cho, Sung Joo Hwang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A rapid, sensitive method of detecting sirolimus in blood was developed and applied in pharmacokinetic studies employing deionized water for hemolysis and a weakly basic mobile phase to enhance chromatographic peak intensity. Dog blood samples were processed via liquid-liquid extraction and the amounts of sirolimus and tacrolimus, an internal standard, were quantified by LC-MS/MS. Specificity, the lower limit of quantification, linearity, accuracy, precision, dilution, recovery, matrix effects, robustness and stability were within the acceptable range for assay validation. The concentration of sirolimus was quantifiable in blood samples for up to 36 h after the dog had received a 3 mg/kg dose of sirolimus. These observations suggest that sirolimus can be detected at low levels in dog blood using a basic mobile phase and metal-free hemolysis. This method is therefore applicable to pharmacokinetic studies in dogs.

Original languageEnglish
Pages (from-to)1042-1047
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume53
Issue number4
DOIs
Publication statusPublished - 2010 Dec 1

Fingerprint

Pharmacokinetics
Liquid chromatography
Sirolimus
Tandem Mass Spectrometry
Liquid Chromatography
Mass spectrometry
Blood
Dogs
Hemolysis
Liquid-Liquid Extraction
Deionized water
Liquids
Tacrolimus
Dilution
Assays
Metals
Recovery
Water

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Cite this

@article{085d456bea2e4741bfdc0b4500cc7d14,
title = "Quantitative determination of sirolimus in dog blood using liquid chromatography-tandem mass spectrometry, and its applications to pharmacokinetic studies",
abstract = "A rapid, sensitive method of detecting sirolimus in blood was developed and applied in pharmacokinetic studies employing deionized water for hemolysis and a weakly basic mobile phase to enhance chromatographic peak intensity. Dog blood samples were processed via liquid-liquid extraction and the amounts of sirolimus and tacrolimus, an internal standard, were quantified by LC-MS/MS. Specificity, the lower limit of quantification, linearity, accuracy, precision, dilution, recovery, matrix effects, robustness and stability were within the acceptable range for assay validation. The concentration of sirolimus was quantifiable in blood samples for up to 36 h after the dog had received a 3 mg/kg dose of sirolimus. These observations suggest that sirolimus can be detected at low levels in dog blood using a basic mobile phase and metal-free hemolysis. This method is therefore applicable to pharmacokinetic studies in dogs.",
author = "Lee, {Jong Hwa} and Cha, {Kwang Ho} and Wonkyung Cho and Junsung Park and Park, {Hee Jun} and Youngseok Cho and Hwang, {Sung Joo}",
year = "2010",
month = "12",
day = "1",
doi = "10.1016/j.jpba.2010.06.030",
language = "English",
volume = "53",
pages = "1042--1047",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
issn = "0731-7085",
publisher = "Elsevier",
number = "4",

}

Quantitative determination of sirolimus in dog blood using liquid chromatography-tandem mass spectrometry, and its applications to pharmacokinetic studies. / Lee, Jong Hwa; Cha, Kwang Ho; Cho, Wonkyung; Park, Junsung; Park, Hee Jun; Cho, Youngseok; Hwang, Sung Joo.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 53, No. 4, 01.12.2010, p. 1042-1047.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Quantitative determination of sirolimus in dog blood using liquid chromatography-tandem mass spectrometry, and its applications to pharmacokinetic studies

AU - Lee, Jong Hwa

AU - Cha, Kwang Ho

AU - Cho, Wonkyung

AU - Park, Junsung

AU - Park, Hee Jun

AU - Cho, Youngseok

AU - Hwang, Sung Joo

PY - 2010/12/1

Y1 - 2010/12/1

N2 - A rapid, sensitive method of detecting sirolimus in blood was developed and applied in pharmacokinetic studies employing deionized water for hemolysis and a weakly basic mobile phase to enhance chromatographic peak intensity. Dog blood samples were processed via liquid-liquid extraction and the amounts of sirolimus and tacrolimus, an internal standard, were quantified by LC-MS/MS. Specificity, the lower limit of quantification, linearity, accuracy, precision, dilution, recovery, matrix effects, robustness and stability were within the acceptable range for assay validation. The concentration of sirolimus was quantifiable in blood samples for up to 36 h after the dog had received a 3 mg/kg dose of sirolimus. These observations suggest that sirolimus can be detected at low levels in dog blood using a basic mobile phase and metal-free hemolysis. This method is therefore applicable to pharmacokinetic studies in dogs.

AB - A rapid, sensitive method of detecting sirolimus in blood was developed and applied in pharmacokinetic studies employing deionized water for hemolysis and a weakly basic mobile phase to enhance chromatographic peak intensity. Dog blood samples were processed via liquid-liquid extraction and the amounts of sirolimus and tacrolimus, an internal standard, were quantified by LC-MS/MS. Specificity, the lower limit of quantification, linearity, accuracy, precision, dilution, recovery, matrix effects, robustness and stability were within the acceptable range for assay validation. The concentration of sirolimus was quantifiable in blood samples for up to 36 h after the dog had received a 3 mg/kg dose of sirolimus. These observations suggest that sirolimus can be detected at low levels in dog blood using a basic mobile phase and metal-free hemolysis. This method is therefore applicable to pharmacokinetic studies in dogs.

UR - http://www.scopus.com/inward/record.url?scp=77955587164&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955587164&partnerID=8YFLogxK

U2 - 10.1016/j.jpba.2010.06.030

DO - 10.1016/j.jpba.2010.06.030

M3 - Article

C2 - 20674213

AN - SCOPUS:77955587164

VL - 53

SP - 1042

EP - 1047

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

IS - 4

ER -